Recombinant Human CD300A Protein (Fc Tag)

Name: Recombinant Human CD300A Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-0869
Availability: InStock

Collections: Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Human
Accession	Q9UGN4
Synonym	CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60
Background	CMRF35-like molecule 8, also known as CD3 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 6, NK inhibitory receptor, CD3a and CMRF35H, is a single-pass type I membrane protein which belongs to theCD3 family. The CD3 family of myeloid immunoglobulin receptors includes activating ( CD3b, CD3e ) and inhibitory members ( CD3a, CD3f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD3c, CD3d ). CD3A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains oneIg-like V-type ( immunoglobulin-like ) domain. CD3A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD3c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD3c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD3a and CD3c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).
Description	A DNA sequence encoding the human CD300A (Q9UGN4-1)(Met1-Gln178) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Leu 18
AA Sequence	Met1-Gln178
Molecular Weight	The recombinant human CD300A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 a.a. and has a predicted molecular mass of 44.5 kDa. The apparent molecular mass of the protein is approximately 58-66 kDa in SDS-PAGE under reducing conditions.
Purity	(97.9+1.1)% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.