Recombinant Human CD96 Protein (His Tag)

Name: Recombinant Human CD96 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1163
Availability: InStock

Collections: Cluster of differentiation (cd) proteins, Co-inhibitory receptors, Featured cd protein molecules, Featured immune checkpoint protein molecules, Immune checkpoint proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P40200
Synonym	CD96 antigen
Background	CD96 (Cluster of Differentiation 96) or Tactile (T cell activation, increased late expression) is a receptor expressed on T cells and NK cells, and shares sequence similarity with CD226 (also known as DNAM-1). The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesion of activated T and NK cells to their target cells during the late phase of the immune response. It may also function in antigen presentation[citation needed]. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. CD96 is a transmembrane glycoprotein that has three extracellular immunoglobulin-like domains and is expressed by all resting human and mouse NK cells. CD96 main ligand is CD155. CD 96 has approximately 20% homology with CD226 and competed for binding to CD155 with CD226.
Description	A DNA sequence encoding the human CD96 isoform 2 (P40200-2) extracellular domain (Met 1-Met 503) was produced, with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Val 22
AA Sequence	Met 1-Met 503
Molecular Weight	The recombinant human CD96 consists of 493 amino acids and predictes a molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD96 is approximately 120-130 kDa due to high glycosylation.
Purity	Greater than 90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its ability to bind recombinant mouse PVR in a functional ELISA.2. Measured by its ability to bind recombinant Human CD155 in a functional ELISA.
Formulation	Lyophilized from sterile PBS, pH 7.4
Stability	Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C
Usage	For Research Use Only
Storage	Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.