Recombinant Human EGFL6 Protein (His & FLAG Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1720
Recombinant Human EGFL6 Protein (His & FLAG Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1720
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&FLAG |
Host Species | Human |
Accession | NP_056322.2 |
Synonym | MAEG, W80 |
Background | EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors. |
Description | A DNA sequence encoding the human EGFL6 (NP_056322.2) (Asn22-Asp553) was expressed with the flag tag at the N-terminus and the histidine tag at the C-terminus. |
Source | Baculovirus-insect cells |
Predicted N Terminal | Met |
AA Sequence | Asn22-Asp553 |
Molecular Weight | The recombinant human EGFL6 consists of 551 a.a. and has a calculated molecular mass of 61.5 kDa. |
Purity | >65% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 x 10E4 cells/well areadded to CD4-coated plates (1.25ug/mL and 100uL/well),approximately 40%-60% will adhere specifically after 30 minutes at 37°C. |
Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |