Recombinant Human FGF-10 Protein

Name: Recombinant Human FGF-10 Protein
Brand: Beta LifeScience
SKU: BL-1955NP
Availability: InStock

BL-1955NP: Greater than 95% as determined by reducing SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

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Product Overview

Description	Recombinant Human Fibroblast Growth Factor 10 is produced by our E.coli expression system and the target gene encoding Gln38-Ser208 is expressed.
Accession	O15520
Synonym	Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2
Gene Background	Fibroblast growth factor 10 (FGF-10, KGF-2), is a member of the fibroblast growth factor (FGF) family that includes FGF-3, -7, and -22. KGF-2 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGFR2 and interacts more weakly with FGFR1. It plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. It exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is required for normal branching morphogenesis. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
Molecular Mass	19.5 KDa
Apmol Mass	19-22 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 8.0.
Endotoxin	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.
Subcellular Location	Secreted.
Protein Families	Heparin-binding growth factors family
Database References	HGNC: 3666 OMIM: 149730 KEGG: hsa:2255 STRING: 9606.ENSP00000264664 UniGene: Hs.248049
Associated Diseases	Aplasia of lacrimal and salivary glands (ALSG); Lacrimo-auriculo-dento-digital syndrome (LADDS)

Gene Functions References

We report the ophthalmological and genetic study of a 19-year-old woman and her relatives suffering from this syndrome. A new probably pathogenic variant is described in the FGF10 gene. PMID: 29053399
We have revealed significant association of FGFR2 and MAP3K1 polymorphisms with breast cancer. PMID: 29372690
data suggest that the strongest signal of association at 5p12 is mediated through coordinated activation of FGF10 and MRPS30, two candidate genes for breast cancer pathogenesis PMID: 27640304
Fgf10 signaling has an essential role in the formation of lipofibroblasts during late lung development PMID: 26511927
Expression of Fibroblast Growth Factor 10 is correlated with poor prognosis in gastric adenocarcinoma. PMID: 26268776
FGF10 has a role in protecting neuron against oxygen-glucose deprivation injury through inducing heme oxygenase-1 PMID: 25446127
Data identify autocrine activation of FGF signaling as an essential mechanism in promoting Pten-deficient skin tumors. PMID: 24582960
The therapeutic potential of the FGF10 treatment. PMID: 24865969
FGF10 plays an important role for tumor growth by both paracrine and autocrine manner. PMID: 24320134
The findings show that immunohistochemistry with FGF10, FGFR2b, or SHH could be useful in differentiating CCAM from type I PPB, when a child presents with a focal cystic lung lesion. PMID: 24004862
Paracrine FGF10 signaling stimulates the differentiation of human stem cell into urothelial cells. PMID: 23949743
High FGF10 expression is associated with ameloblastoma. PMID: 24002438
Three FGF10 single nucleotide polymorphisms in complete linkage disequilibrium--rs339501, rs12517396, and rs10462070--were associated with extreme myopia in the Japanese population. PMID: 24265547
FGF-10 expression during the development of the human hindgut and anorectum suggests that it may play a role in hindgut and anorectal morphogenesis. PMID: 23774963
The sclera of myopic eyes had higher FGF10 levels. The risk G allele of SNP rs339501 was associated with extreme myopia in human and caused a higher gene expression in the luciferase assay. PMID: 23599340
the posttranslational and transcriptional mechanisms underlying stimulation of P-glycoprotein function and expression by keratinocyte growth factor-2 (KGF2) that may contribute to the beneficial effects of KGF2 in intestinal inflammatory disorders PMID: 23328208
tooth agenesis had increased risk of a family history of cancer. tooth agenesis was associated with positive self-reported family history of cancer and variants in FGF10. PMID: 23169889
Identification of FGF-10 at both protein and mRNA levels in ovaries from fetuses, girls, and women suggests that FGF-10 contributes to preantral follicle development; FGF-10 is expressed in both granulosa cells and oocytes. PMID: 22877940
Our data suggest that common variants in FGF10 increase the risk for a wide range of non-syndromic limb deficiencies. PMID: 22965740
[review] FGF10 mediates biological responses by activating FGF receptor 2b (FGFR2b) in a paracrine manner. PMID: 21696361
The relationship between human ISL1 and FGF10 within the embryonic time window during which the linear heart tube remodels into four chambers, was examined. PMID: 22303449
Investigation of the pulmonary functions of COPD patients heterozygous for loss of function mutations in the FGF10 gene was performed. The patients show a significant decrease in lung function parameters when compared to control values. PMID: 21742743
Gremlin-mediated BMP inhibition results in activation of epithelial cells and transient fibrosis, but also induction of epithelium-protective FGF10 PMID: 20705941
FGF2 and FGF10 regulate migratory activity of ovine trophoblast cells through MAPK-dependent pathways. PMID: 21310815
FGF10 can promote the adipogenesis effect in situ. PMID: 19915940
There was no association among gene FGFR1 rs13317, p. E467K, p. M369I, p. S393S and gene FGF10 rs1448037 and nonsyndromic cleft lip with or without palate in Chinese population. PMID: 19727229
These results suggested that Thr-114 is a crucial functional residue for FGF10, and mutating Thr-114 to Ala or Arg would lead to great decrease in receptor-binding affinity and biological activity of FGF10. PMID: 20036575
FGF10 upregulates Na(+)-K(+)-exchanging ATPase via the MAPK pathway. PMID: 12804770
FGF-10 attenuates H2O2-induced alveolar epithelium DNA damage by mechanisms that involve activation of Grb2-SOS/Ras/RAF-1/ERK1/2 pathway and DNA repair. PMID: 14975937
Fgf10 mRNA is overexpressed in a subset of human breast carcinomas. PMID: 15208658
Data validate the symmetric two-end model of fibroblast growth factor (FGF) receptor (FGFR) dimerization and FGF binding and argue against the asymmetric model of FGFR dimerization. PMID: 15632068
We suggest that haploinsufficiency for FGF10 during a crucial stage of development results in ALSG. PMID: 15654336
Human embryonic pancreatic mesenchyme expresses FGF10, which might be used used to expand human embryonic pancreatic epithelial cells. PMID: 15690149
Human recombinant FGF10 maintains murine Notch activation and induces the expansion of murine pancreatic precursors while blocking their differentiation. PMID: 16323074
Localization of the FGF-10 receptor to the urothelial layer is clinically significant because intravesical administration of FGF-10 may provide a means to control the turnover of transitional epithelium in bladder disorders such as interstitial cystitis. PMID: 16597614
Activation of Toll-like receptor 2 (TLR2) or Toll-like receptor 4 (TLR4) inhibited FGF-10 expression, leading to abnormal saccular airway morphogenesis. PMID: 17071719
R80S and G138E mutations are associated with aplasia of the lacrimal and salivary glands. PMID: 17213838
a nuclear localization signal-like motif of FGF-10 is a partial determinant of its intracellular distribution and is necessary for its mitogenic activity PMID: 17471512
Trophoblast outgrowth and invasion (part of placental villi sprouting) at the fetal maternal interface is in part under delicate control of FGF 10 and Sprouty 2. PMID: 17496316
Results suggest that Lacrimo-auriculo-dento-digital syndrome is caused by reduced activity of the fibroblast growth factor 10 (FGF10)-FGF receptor 2 signaling pathway. PMID: 17682060
Expression in diverse niches of adult brain of Fgf10-lacZ reporter mice implicates Fgf10 in control of neurogenesis and/or conservation of neurogenic potential. PMID: 18329286
It cannot be ruled out that other genes involved in the signalling pathway of FGF10 may contribute to the formation of these congenital malformations. PMID: 18587586
Stromal FGF10 induces migration and invasion in pancreatic cancer cells through interaction with FGFR2, resulting in a poor prognosis. PMID: 18594526
family-based approach revealed an intronic variation of the FGF10 gene causing aplasia of lacrimal and salivary glands-syndrome PMID: 19102732
Disruption of localized pattern of mesenchymal fibroblast growth factor 10 expression results in impairment of lung branching morphogenesis, which progresses to emphysematous airspaces in adults. PMID: 19115389
Fgf10 is a strong causative candidate for defects observed in Apert syndrome since its genetic knockdown in a mouse model of Apert syndrome results in the rescue of the skeletal and visceral defects associated with this congenital disease. PMID: 18773495
An analysis of the distribution and fate of Fgf10-expressing cells in the adult mouse brain suggests that Fgf10 may have critical regulatory roles in stem cell function and generation of new neurons in diverse areas of the adult brain. PMID: 18773495
TGFbeta1 caused a rapid and transient decrease in Fgf10 mRNA levels in primary prostatic cells. Deletion analysis of the Fgf10 promoter identified a region that mediated a proportion of promoter activity as well as promoter down-regulation by TGFbeta1. PMID: 14726452

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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