Recombinant Human FGF18 Protein (His Tag)

Name: Recombinant Human FGF18 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2039
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag	His
Host Species	Human
Accession	O76093
Synonym	FGF18, FGF-18, ZFGF5, fibroblast growth factor 18
Background	Fibroblast growth factor 18 is a protein that in humans is encoded by the FGF18 gene. FGF18 (Fibroblast Growth Factor 18) is a Protein Coding gene. Diseases associated with FGF18 include Dyslexia. Among its related pathways are Signaling by activated point mutants of FGFR3 and PI3K/AKT activation. Gene Ontology (GO) annotations related to this gene include growth factor activity and type 2 fibroblast growth factor receptor binding. An important paralog of this gene is FGF8.
Description	A DNA sequence encoding the human FGF18 (Met 1-Ala 207) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Glu 28
AA Sequence	Met 1-Ala 207
Molecular Weight	The secreted recombinant human FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.3 kDa. The apparent molecular mass of rh FGF18 is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>92% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA.2. Immobilized human FGF18 at 10 ug/mL (100 ul/well) can bind- Rat FGFR4, The EC50 of Rat FGFR4 is 1.17 ug/mL.
Formulation	Recombinant Human FGF18 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Human FGF18 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.
Subcellular Location	Secreted.
Protein Families	Heparin-binding growth factors family
Database References	HGNC: 3674 OMIM: 603726 KEGG: hsa:8817 STRING: 9606.ENSP00000274625 UniGene: Hs.87191

Gene Functions References

FGF18 played an important role in the growth and metastasis of breast cancer. PMID: 29901199
we genotyped rs9313548 and performed case-control-based association analysis with developmental dyslexia in a Chinese population. Our results were in consistent with previous studies as we did not observe significant association of rs9313548 with developmental dyslexia. PMID: 29240020
the suppressive effect of miR-139 on FGF18 and in turn on proliferation, apoptosis, invasion, migration and tumor-induced angiogenesis of HCC cells was investigated. FGF18 was suggested as a prognostic biomarker and therapeutic target in HCC patients and miR-139 may be a promising strategy used in HCC treatment via the suppression of FGF18 PMID: 28765917
the results suggest that FGF18 may be involved in MC3T3E1 cell proliferation and osteoblastic differentiation. PMID: 28765932
significantly increased in endometrioid adenocarinoma PMID: 27267714
FGF9 and FGF18 increased the migratory capacities of human lung fibroblasts, and FGF9 actively modulated matrix metalloproteinase activity in idiopathic pulmonary fibrosis. PMID: 26773067
FGF18 serves an essential role in the growth and migration of non-small cell lung cancer cells by regulating the ERK, p38 signaling pathways and MMP26 protein levels. PMID: 27959447
Data suggest that the combination of FIGO stage, ovarian carcinoma type, and/or fibroblast growth factor 18 (FGF18) score could predict poor prognosis among ovarian carcinoma patients. PMID: 26427667
The position of sulfate ions bound to FGF18 provides insight into the putative HS-binding site and allows comparison with the prototypical FGFs, FGF1, and FGF2. PMID: 24668462
role for FGF-18 in chondrogenic and osteogenic events which drive discal development and ossification of the vertebral bodies. PMID: 23397188
Fgf18 as a molecule that protects articular cartilage by gene expression profiling, and the anticatabolic effects may at least partially be mediated by the Timp1 expression. PMID: 24577103
Tumors from ovarian cancer patients had increased FGF18 expression levels with microvessel density and M2 macrophage infiltration. PMID: 24018557
FGF8, FGF17, and FGF18 are involved in autocrine and paracrine signaling in HCC and enhance the survival of tumor cells under stress conditions, malignant behavior, and neoangiogenesis. PMID: 21319186
There was an association between gene FGF18 rs4043716 and nonsyndromic cleft lip with or without palate in Chinese population. PMID: 19727229
FGF2 and -18 bind to discrete structures on the heparan sulfate chains attached to chondrocyte-derived perlecan which modulate the growth factor activities PMID: 20507176
fibroblast growth factor 18 seems to play a role in maintenance of chondrocyte properties, although its expression was rather high in dedifferentiated chondrocytes. PMID: 19909293
analysis of FGF18 and FGFR5 expression in primary endothelial cells and vascular smooth muscle cells PMID: 16019430
FGF18 is wildly expressed in the cardiovascular tissue. It enhanced cell migration in response to mechanical damage. we detected FGF18 expression in liver vascular and liver sinusoidal endothelial cells (LSECs), but not in hepatic parenchymal cells. PMID: 16756958
FGF18 may play a prominent role in chondrogenesis and osteogenesis during skeletal development and growth (review) PMID: 17128416
FGF3, FGF7, FGF10, FGF18, and FGFR1 may have roles in nonsyndromic cleft lip and palate PMID: 17360555
FGF18 is almost generally over-expressed in colon cancer and exerts pro-tumorigenic effects both in the epithelial and the stromal compartments by stimulating growth and survival of tumour cells, migration of fibroblasts and neovascularization PMID: 17890768

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.