Recombinant Human FGFBP3 Protein (His Tag)

Name: Recombinant Human FGFBP3 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2052
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	Q8TAT2
Synonym	Fibroblast Growth Factor Binding Protein 3,Fibroblast Growth Factor-Binding Protein 3,FGF-Binding Protein 3, C10orf13,FGF-BP3,FGFBP-3,Chromosome 10 Open Reading Frame 13
Background	Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability. FGFP3_HUMAN,Q8TAT2
Description	A DNA sequence encoding the human FGFBP3 (Q8TAT2) was expressed, with a C-terminal His tag.
Source	Insect Cells
Predicted N Terminal	Arg 27
AA Sequence	Met 1-Gly 258
Molecular Weight	The secreted recombinant human FGFBP3 consists of 243 amino acids and predicts a molecular mass of 26.4 KDa. The apparent molecular mass of the protein is approximately 33 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>87% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Recombinant Human FGFBP3 Protein (His Tag) was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
Stability	Recombinant Human FGFBP3 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.