Recombinant Human IGFBP4 Protein (His Tag)

Name: Recombinant Human IGFBP4 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2580
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NP_001543.2
Synonym	IGFBP-4 Insulin-Like Growth Factor-Binding Protein 4; IBP-4; IGF-Binding Protein 4; IGFBP-4; IGFBP4; IBP4
Background	Insulin-like growth factor binding protein 4 (IGFBP-4) is a 24 kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. All members of the IGFBP family can bind IGF-I and IGF-II with about equal affinity, but IGFBP-4 binds IGF2 more than IGF1. It contains IGFBP N-terminal domain and thyroglobulin type-1 domain. IGFBP-4 is induced by forskolin and N6, O2-€™dibutyryl sdenosine 3-€™, or 5-€™-cyclic monophosphate. The IGF-binding proteins can prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Description	A DNA sequence encoding the human IGFBP4 (NP_001543.2) precursor (Met 1-Glu 258) with a C-terminal His tag was expressed.
Source	HEK293
Predicted N Terminal	Asp 22
AA Sequence	Met 1-Glu 258
Molecular Weight	The secreted recombinant human IGFBP4 comprises 248 amino acids with a predicted molecular mass of 27.4 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its ability to bind human IGF1 in functional ELISA.2. Measured by its ability to bind human IGF2 in functional ELISA.3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.04-0.4 ug/mL in the presence of 14 ng/mL human IGFII.
Formulation	Recombinant Human IGFBP4 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Human IGFBP4 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.