Recombinant Human IL-17RA Protein (C-6His)

Name: Recombinant Human IL-17RA Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2677NP
Availability: InStock

BL-2677NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-17 Receptor A is produced by our Mammalian expression system and the target gene encoding Leu33-Trp320 is expressed with a 6His tag at the C-terminus.
Accession	Q96F46
Synonym	IL17R; interleukin-17 receptor A; interleukin 17 receptor A; IL17RA;CD217
Gene Background	CD217, also known as Interleukin-17 receptor A, is a T cell expressed pleotropic cytokine with 866 amino acids in length. CD217 functions as receptors for IL17A and IL17F, it binds to IL17A with higher affinity than to IL17F and binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC. It also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. The activation of IL17RA would lead to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. The signaling events of IL 17 includes activation of NF kappa B and JNK, and require TNF receptor associated factors 6 (TRAF6) in the signaling pathway.
Molecular Mass	34.5 KDa
Apmol Mass	50-80 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Receptor for IL17A and IL17F, major effector cytokines of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Receptor for IL17A. Receptor for IL17F. Binds to IL17A with higher affinity than to IL17F. Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC. Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. Cytokine binding triggers homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways, ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Involved in antimicrobial host defense primarily promoting neutrophil activation and recruitment at infection sites to destroy extracellular bacteria and fungi. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Plays a role in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms. Enhances immunity against West Nile virus by promoting T cell cytotoxicity. Contributes to Influenza virus clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense. Receptor for IL17C as part of a heterodimeric complex with IL17RE.; (Microbial infection) Receptor for SARS coronavirus-2/SARS-CoV-2 virus protein ORF8, leading to IL17 pathway activation and an increased secretion of pro-inflammatory factors through activating NF-kappa-B signaling pathway.
Subcellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Database References	HGNC: 5985 OMIM: 605461 KEGG: hsa:23765 STRING: 9606.ENSP00000320936 UniGene: Hs.48353
Associated Diseases	Immunodeficiency 51 (IMD51)
Tissue Specificity	Widely expressed.

Gene Functions References

The SNP rs4819554 in the promoter region of IL17RA significantly influences the response to anti-TNF drugs at week 12. PMID: 27670766
Combination of polymorphisms in the NOD2, IL17RA, EPHA2 and KALRN genes could play a significant role in the development of sarcoidosis by maintaining a chronic pro-inflammatory status in macrophages PMID: 29554915
The data connect increased interleukin (IL)-17A/IL-17RA signaling in autism spectrum disorder patients with enhanced oxidative inflammation in monocytes. PMID: 28935156
IL-17RA is essential for mucocutaneous immunity to Candida and Staphylococcus, but otherwise largely redundant PMID: 27930337
Here, we discuss the latest discoveries on the role of the IL-17A/IL-17RA axis in driving host pulmonary defence and immunopathology. PMID: 27033174
High IL-17RA expression is associated with prostate cancer. PMID: 26871944
Expression of these mediators was confirmed in end-stage COPD. Thus, accumulation of mast cells in COPD may contribute to vascular remodeling. PMID: 28298222
cyanidin specifically recognizes an IL-17A binding site in the IL-17A receptor subunit (IL-17RA) and inhibits the IL-17A/IL-17RA interaction. PMID: 28223414
SNP rs4819554 in promoter is associated with functional severity of ankylosing spondylitis PMID: 27415816
It has been shown that IL-17 can be produced by enteroendocrine chromogranin A-positive cells as well as by goblet cells in the human colon. PMID: 27660002
Results suggest that IL-17-IL-17R interaction in GSCs induces an autocrine/paracrine cytokine feedback loop, which may provide an important signaling component for maintenance/self-renewal of GSCs. PMID: 26755664
High IL-17RA expression is associated with Intrahepatic Cholangiocarcinoma. PMID: 26228109
Identified the IL17RA rs4819554 SNP as a risk factor for psoriasis. PMID: 26347322
IL-17 receptor A and adenosine deaminase 2 deficiency due to deletion mutations was found in siblings with chronic mucocutaneous candidiasis and chronic systemic inflammation. PMID: 26607704
In vitro, CSE stimulation significantly increased IL-17F and IL-17R in 16HBE (2.5%) and A549 (5%) while IL-17A and IL-17F in PBMC (10%). IL-17A and CSE stimulation, rather than CSE or rhIL-17A alone, increased proliferation in 16HBE and apoptosis in A549 PMID: 26198032
The data indicate that the genetic variants of rs2275913 in IL-17A, rs763780 in IL-17F, and rs4819554 in IL-17RA may not play a role in the pathogenesis of preeclampsia in Chinese Han women. PMID: 26451724
IL17F and IL17RA polymorphisms modulate susceptibility to cerebral malaria(CM) and provide evidence that IL-17F protects against CM. PMID: 26667835
Increased expression of IL-17RA plays an important role in gastric cancer progression, migration and prognosis. PMID: 26261590
Both genetic variants of IL-17R (rs882643 and rs2241049) were associated with PGD. This confirms a genetic predisposition toward PGD and suggests a role of IL-17 in driving neutrophilia in PGD. PMID: 25935436
The presence of IL7R polymorphisms seems to be related to severe liver disease in HIV/hepatitis c virus coinfected patients. PMID: 26123260
expression of immunoreactivity for IL-17A, IL-17RA, IL-17E, and IL-17F was significantly elevated in prostatic tissue from benign prostatic hyperplasia and prostate cancer compared with that in controls PMID: 26356122
genetic polymorphism is associated with a reduced renal filtration rate, and with the risk of developing end stage renal disease in healthy elderly from Spain PMID: 25636567
IL17RA polymorphisms, but not those of IL17, can influence both the development and the bilaterality of papillary thyroid carcinoma PMID: 25484349
IL-17R was almost exclusively expressed by endothelial cells, not by myeloma cells in the masses of skeletal extramedullary disease patients. PMID: 24916639
Interleukin-17 receptor A crystal structure shows that the downstream motif of IL-17RA SEFIR together with helix alpha C could provide a composite ligand-binding surface for recruiting Act1 during IL-17 signaling. PMID: 24816115
IL17-dependent retinal degeneration in a mouse model of focal retinal degeneration can be prevented by gene therapy with adeno-associated virus vector encoding soluble IL17 receptor PMID: 24780906
Whereas no association was found between the alternative splicing SNP, rs6897932, and multiple sclerosis, a significant link was found between the promoter single nucleotide polymorphism, rs11567685, and the disease. PMID: 24166352
Genetic variation in interleukin-17 receptor A is functionally associated with chronic rejection after lung transplantation. PMID: 24263024
Data show that the interleukin (IL)-17 receptor IL-17RA variant is generated by spliced out of exon 11 encoding the transmembrane region in a variety of tissues. PMID: 24084331
The increased IL-25 levels in plasma and the expression of IL-17RA and IL-17RB on eosinophils in allergic asthma patients suggests that IL-25 may activate eosinophils during allergic inflammation. PMID: 24247484
crystal structures of homodimeric IL-17A and its complex with IL-17RA, are reported. PMID: 23695682
Il-17R SNPs were not associated with psoriatic arthritis susceptibility in northern Italians. PMID: 22955875
polymorphism of IL-17R plays no significant role in incidence of chronic periodontitis and Periimplantitis. PMID: 23852838
Our results might be helpful for explaining the missing heritability of the psoriasis due to epistasis and provide a deep insight into the important role of the IL23/Th17 pathway in the pathogenesis of psoriasis. PMID: 22909235
Data suggest that the interleukin 17 receptor A (IL17RA) minor alleles of the three single nucleotide polymorphisms (SNP) may decrease the risk of Aspirin Exacerbated Respiratory Disease (AERD) via attenuation of IL17RA gene expression. PMID: 23220496
IL-17A/IL-17RA interaction promoted the metastasis of OS in nude mice. PMID: 23192273
Our data indicates that serum IL-17 levels are significantly increased in intractable Graves disease and affected thyrocytes show functional IL-17R expression. PMID: 23501056
Two polymorphisms within the IL17E and IL17RA genes are associated with ESRD independent of age and sex. PMID: 23147652
Data show that IL-17RA, IL-17RC, IL-22R1, ERK1/2 MAPK and NF-kappaB pathways are involved in Th17 cytokine-induced proliferation. PMID: 22898922
protein levels of IL-17 and IL-17R positively correlated with the frequency of seizures in focal cortical dysplasias patients patients. PMID: 23334598
IFN-alpha could be associated with the expansion of IL-7Ralpha(low) CD45RA(+) EM CD8(+) T cells in the CMV-uninfected elderly PMID: 22484243
Blockage of IL-17RA with an anti-IL-17RA antibody inhibited the production of IL-6, IL-8, and MMP-3; demonstrate the functional significance of IL-17RA in psoriatic arthritis PMID: 21894442
mRNA levels of IL-17, IL-17R and MMP-9 were also higher in the invasive group than in the non-invasive group in human pituitary adenomas. PMID: 21279695
IL-17/IL-17RA signaling plays an important role in myocardial collagen metabolism in hypertension-induced diastolic dysfunction. PMID: 21530504
IL-17 may have an important role in the occurrence of nasal polyps by specific combination with IL-17R and over-expression in nasal polyps. PMID: 16874957
Human periodontal ligament fibroblasts are a target of Th17, and that IL-17 appears to up-regulate the expression of IL-23 p19 via a homeostatic mechanism involving Akt-, p38 MAPK-, and ERK 1/2-dependent NF-kappaB signalling versus the JNK/AP-1 pathway. PMID: 21145111
an extended SEFIR domain is required for il-17RA-mediated signal transduction. PMID: 20729198
Expression, modulation and signalling of IL-17 receptor in fibroblast-like synoviocytes of patients with rheumatoid arthritis PMID: 11966773
cell membrane IL-17R is required for signaling by both IL-17A and IL-17F PMID: 15972674
In this JEG-3 cell model of human trophoblast, the IL-17 receptor may have a regulatory role in trophoblast invasion. PMID: 16533341

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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