Recombinant Human IL-10 Protein

Name: Recombinant Human IL-10 Protein
Brand: Beta LifeScience
SKU: BL-1850NP
Availability: InStock

BL-1850NP: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-10 is produced by our Mammalian expression system and the target gene encoding Ser19-Asn178 is expressed.
Accession	P22301
Synonym	Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10; RP11-262N9.1; IL10A; MGC126450; MGC126451; TGIF
Gene Background	Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations
Molecular Mass	18.6 KDa
Apmol Mass	16 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Subcellular Location	Secreted.
Protein Families	IL-10 family
Database References	HGNC: 5962 OMIM: 124092 KEGG: hsa:3586 STRING: 9606.ENSP00000412237 UniGene: Hs.193717
Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Gene Functions References

Autocrine IL10 regulates Op18/stathmin signaling via an IL10NFkappaBERK/CDC2 axis. PMID: 30320402
CD163(+)CD204(+) Tumor-associated macrophages possibly play a key role in the invasion and metastasis of oral squamous cell carcinoma by T-cell regulation via IL-10 and PD-L1 production. PMID: 28496107
study showed that the rs1800872 A allele of the IL-10 gene may contribute to the genetic susceptibility of Behcet's disease by regulating the expression of IL-10. PMID: 29294320
study indicates that anti-inflammatory macrophage function and mucosal immune tolerance require both WASP and DOCK8, and that IL-10 signalling modulates a WASP-DOCK8 complex PMID: 29725003
This study demonstrated the alteration of IL-10 levels in aseptic non-vasculitic cerebral sinovenous thrombosis PMID: 30246697
The interleukin-10 gene promoter rs1800872 single nucleotide polymorphism is associated with predisposition to chronic hepatitis C in case of infection with hepatitis C virus 1b genotype in the Russian population. PMID: 29247709
Mycobacterium tuberculosis infection disturbs the HDAC6/HDAC11 levels to induce IL-10 expression in macrophages. PMID: 29523311
The results of this meta-analysis indicated that there is no association between the IL-10 -592 A/C promoter polymorphism and gastrointestinal tract cancer susceptibility. PMID: 29720026
Low IL10 expression is associated with dyslipidaemia, high blood pressure and glucose intolerance. PMID: 29615317
Regulatory polymorphism in the IL-10 gene promoter has a possible and significant association with severity and outcome in patients with acute viral hepatitis, and acute liver failure PMID: 30109600
These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis. PMID: 28216665
Individuals with posttraumatic stress disorder showed a significant increase in the serum levels of IL-10 (and IL-6). PMID: 29179015
IL-10 SNP at -819 was associated with enhanced AML risk, suggesting that rs1800871 provides clue for future studies and early detection of Acute myeloid leukemia . PMID: 30197353
Although IL-10 promoter polymorphisms are not associated with P. falciparum and S. haematobium co-infection, variant -1082G/A and haplotype GCC are associated with malaria, whereas the IL-10 haplotypes GCC and GTA are associated with schistosomiasis. PMID: 29131459
high IL-10 in rheumatoid arthritis is associated with increased depressive symptoms PMID: 30148175
besides the NF-kappaB pathway, the mechanism of action of FGF-21 was observed to involve the elevation of IL-10 in the ERK1/2 pathway. PMID: 29427162
meta-analysis strongly suggests that the IL10 rs1800896, rs1800871, and rs1800872 polymorphisms are not associated with the risk of autoimmune liver disease PMID: 29694797
Single nucleotide polymorphisms (SNPs) rs12979860 and rs8099917 (IL28B) and rs1800896, rs1800871, and rs1800872 (IL10) are related to treatment outcome, but previous studies clustered nonresponse and relapse patients.Frequency of rs12979860 and rs8099917 is different between relapsers and nonresponders, but similar between relapsers and responders PMID: 29888255
The aim of this study was to explore the possible correlations of serum interleukins and soluble ST2 (sST2) protein with clinical features and inflammatory cytokines in rheumatoid arthritis (RA) patients. PMID: 29798971
Anti-inflammatory cytokines, IL-10 and TGF-beta1, are not upregulated in the serum of patients with juvenile idiopathic arthritis. PMID: 29494710
Overall, no significant association between IL-10 -592 A/C polymorphism and Kawasaki disease risk was found under allele contrast. The IL-10 -592 A/C polymorphism was not associated with Kawasaki disease risk in the Chinese population. PMID: 29656715
These results suggest that the IL-10 -1082 G/A polymorphism confers susceptibility to juvenile idiopathic arthritis. PMID: 29748155
Data show that multiple sclerosis TH1/17 cells have reduced expression of interleukin 10 (IL10) compared to healthy controls. PMID: 29150604
High IL10 and Tumor necrosis factor-alpha expression is associated with coronary artery disease. PMID: 28988596
Results discovered that plasma IL-10 levels in acute respiratory distress syndrome patients were correlated with severity of illness during extracorporeal membrane oxygenation institution. Also, high interleukin-10 was found to be associated to two polymorphic nucleotides at the gene promoter area. PMID: 28432351
association of gene polymorphism (-819C > T, -592C > A and -1082G > A) with preterm birth PMID: 29147889
Suggest that the IL-10 -819T/T variant and the ATA haplotype, which are associated with low production of IL 10, represent genetic risk factors for preeclampsia in Tunisian women. PMID: 29523264
The IL-10 rs1800890 variant might contribute to RA susceptibility in the Chinese population. PMID: 29487192
findings support the hypothesis that the -1082A>G polymorphism in the IL10 gene might be associated with diabetic kidney disease in white Brazilians with type 2 diabetes. PMID: 29227971
This study showed that the activated FcgammaRII(low/-) B cells from HCC tumours, but not the resting FcgammaRII(high) B cells, without external stimulation suppress autologous tumour-specific cytotoxic T-cell immunity via IL-10 signals. PMID: 27853178
It was shown that the expression of CD274, Il-10, and FOXP3 was closely correlated with the clinical characteristics of laryngeal squamous cell carcinoma, including lymph node metastasis and prognosis. PMID: 29257349
IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients.A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. PMID: 27605397
These findings added to the accumulating evidence that promoter haplotypes of IL-10 may be important modulators of the development of amnestic mild cognitive impairment PMID: 29201645
Findings suggest that the combinatorial complexity of TNFA and IL10 promoter polymorphisms impacts systemic lupus erythematosus susceptibility. PMID: 29298134
The combination of IL10 and DAO SNPs in a multivariate model did not alter the OR values. PMID: 28750137
The results indicate that IL10-1082 A/G polymorphism is associated with genetic susceptibility/predisposition to Rheumatoid arthritis in North Indian population. PMID: 29621504
IL-33 increased IL-10 expression in MFCs via activating ERK 1/2 and STAT3, which subsequently promoted IL-10 transcription and thus contributed to the beneficial effects of IL-33 on MFCs. PMID: 29099095
theses results suggest that IL-10 genotypes of recipient are the most associated with the risk of complications after haematopoietic stem cell transplantation PMID: 28987962
Our results suggest that genetic variation in IL-10 gene is unlikely to confer susceptibility to Henoch-Schonlein purpura in Chinese children. PMID: 28963667
neither allele nor genotype frequencies of IL-10 polymorphisms were associated with inflammatory bowel disease in Iranian patients PMID: 28872970
According to our data, TNF A -238G>A and IL-10 -1082A>G, -819C>T and -592C>A may be associated with the development of prostate cancer and BPH. We could also notice higher frequency of TNF A and IL-10 risk haplotypes in smoker and alcohol user. Interestingly, IL-10 risk haplotype was positively associated with aggressiveness of tumor. PMID: 28993831
Local IL-10 and IL-13 upregulation in IgG4-abdominal aortic aneurysms was related to Th2 and Treg-predominant cytokine balance. PMID: 28434701
Suggest that the interaction between macrophages and endometrial stromal cells downregulates cytotoxicity of NK cells possibly by stimulating the secretion of IL-10 and TGF-beta, and may further trigger the immune escape of ectopic fragments and promote the occurrence and the development of endometriosis. PMID: 28971893
isatuximab decreases multiple myeloma cell- and bone marrow stromal cell-induced iTreg by inhibiting both cell-cell contact and TGFbeta/IL10. Finally, CD38 levels correlate with differential inhibition by isatuximab of Tregs from multiple myeloma versus normal donors.Targeting CD38 by isatuximab can preferentially block immunosuppressive Tregs and thereby restore immune effector function against multiple myeloma PMID: 28249894
Activation of PAR2 inhibits the expression of IL-10 in B cells, which can be reversed by treating B cells with Bcl2L12 shRNA-carrying liposomes. PMID: 28426164
Serum IL-17 and IL-23 values positively correlated with serum total IgE levels. Serum levels of IL-10 were lower in children with atopy and dyslipidemia than patients with dyslipidemia. PMID: 28692864
This meta-analysis concludes that IL-10 -1082 gene polymorphism is not significantly associated with overall, Asian and African population. However, this polymorphism is associated with Caucasian population. PMID: 28951522
The data suggest that IL-10, but not IL-37, may have potential as a biomarker predictive for disease activity in Systemic lupus erythematosus. PMID: 27708376
In the study, 250 ONFH patients and 228 matched healthy controls from Shandong Province were recruited to evaluate the influence of interleukin-10 (IL-10) rs1800872, IL-12 rs3212227 and tumor necrosis factor-(TNF-alpha) rs1800629 polymorphism in osteonecrosis of the femoral head (ONFH). IL-12 rs3212227 AC genotype confer genetically susceptibility to ONFH in the Chinese Han population. PMID: 28980933
Gene polymorphisms of TNF-238G/A, TNF-308G/A, IL10-1082G/A, TNFAIP3, and MC4R and comorbidity occurrence in a Romanian population with psoriasis PMID: 29696068

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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