Recombinant Human IL-20 Protein

Name: Recombinant Human IL-20 Protein
Brand: Beta LifeScience
SKU: BL-1807NP
Availability: InStock

BL-1807NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-20 is produced by our E.coli expression system and the target gene encoding Leu25-Glu176 is expressed.
Accession	Q9NYY1
Synonym	Interleukin-20; IL-20; Cytokine Zcyto10; IL20; ZCYTO10
Gene Background	Interleukin-20 (IL-20) is a member of the IL-10 family of regulatory cytokines that includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 exhibits approximately 28% amino acid identity with IL-10 and 76% amino acid identity with mouse IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 Rα and IL-20 Rβ. The second is composed of IL-22 R and IL-20 Rβ. Whereas the IL-22 R/IL-20 Rβ complex is shared with IL-24, the IL-20 Rα/IL-20 Rβ complex is shared with both IL-19 and IL-24. IL-20 has been shown to initiate transduction cascades involving STAT3 and stimulates the induction of pro-inflammatory genes including TNF-α and MCP-1. Initial functional studies using transgenic mice suggest that IL-20 has the ability to regulate skin development. The over-expression of both human and mouse forms of IL-20 results in keratinocyte hyper-proliferation, abnormal epidermal differentiation, and neonatal lethality. In humans, IL-20 and its receptors are up-regulated in psoriatic skin, and polymorphisms in the IL-20 gene have been associated with plaque-type psoriasis. IL-20 may also have a role in hematopoiesis. It enhances the proliferation of multi-potential progenitors in vitro and increases their numbers and cell cycling status in IL-20 transgenic mice. IL-20 is also shown to suppress COX-2 and PGE2 and acts as an inhibitor of angiogenesis in model systems.
Molecular Mass	17.7 KDa
Apmol Mass	17 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3.
Subcellular Location	Secreted.
Protein Families	IL-10 family
Database References	HGNC: 6002 OMIM: 605619 KEGG: hsa:50604 STRING: 9606.ENSP00000356063 UniGene: Hs.272373
Tissue Specificity	Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainl

Gene Functions References

Authors demonstrated that ER(alpha), GATA3 and FOXA1 form a transcriptional complex with Ell3 to regulate IL-20 expression in ER(+) breast cancer cells. FOXA1 represses IL-20 expression, whereas GATA3 and ER(alpha) activate it. PMID: 28514748
The authors results indicate that genetic variants of the immunomodulatory IL10 and IL20 genes may offer a protective effect in Europeans from Russia. PMID: 28150860
data showed that IL-20 is independently associated with rheumatoid arthritis disease activity and may be triggered by TLR ligands at local sites of inflammation PMID: 28662439
IL-20 is a multifunctional cytokine with potent inflammatory, angiogenic, chemoattractive, and osteoclastogenic effects, and is involved in the many phases of rheumatic arthritis progression. [Review] PMID: 26297177
Data suggest that HGF, IL-20, and IL-22 in the serum and bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients before chemotherapy may be a prognostic of cancer progression. PMID: 27573644
The current study provides evidence that IL-20 directly targets and inhibits key inflammatory functions of neutrophils during infection with S. aureus. PMID: 28424238
Results found that IL-20 is more specifically associated with ER-positive breast cancer and is epigenetically regulated by estrogen via the specific recruitment of KMT2B by ERalpha. PMID: 27806114
Coincident diabetes mellitus type 2 in either pulmonary or latent tuberculosis is characterized by decreased production of the IL-20 subfamily of cytokines. PMID: 26354610
IL-20 serum levels were increased in patients with active multiple myeloma compared to both healthy controls and responders to bortezomib-based treatment.IL-20 participate actively in the pathophysiology of multiple myeloma progression. PMID: 25631632
Overexpression of IL-20 was detected in the airway epithelium collected from patients with asthma. PMID: 25028099
IL-20 may play a patho-physiologic role in the Th2 immune response in human asthma and may be a potential biomarker of asthma severity. PMID: 25543042
IL-20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells PMID: 24628979
Decreased interleukin-20 expression in scleroderma skin contributes to cutaneous fibrosis. PMID: 24470401
We observed that IL-20 which is an IL-10 group angiogenesis indicator was suppressed in systemic sclerosis, suggesting abnormal angiogenesis. PMID: 23812620
This study demonstrates that rs1713239 and infection may have potential synergetic effect on modulating the transcriptional activity of IL-20. PMID: 23892591
The mRNA levels of p40, IL-20 and IL-21 at baseline may serve as potential predictors of treatment response to ustekinumab treatment in psoriasis. PMID: 22930279
IL-10-- and IL-20--expressing epithelial and inflammatory cells are increased in patients with ulcerative colitis. PMID: 23207823
the GG genotypes of the IL-20 polymorphisms (rs2981573 and rs2232360) might have an important role in the development of UC in the Mexican population. PMID: 23183096
A blood test for discrimination of patients with acute cellular rejection from transplanted kidney is based on simultaneous quantification of three analytes: IL-1 receptor antagonist, IL-20 and soluble CD40 ligand. PMID: 22948742
Interleukin-20 promotes migration of bladder cancer cells through extracellular signal-regulated kinase (ERK)-mediated MMP-9 protein expression leading to nuclear factor (NF-kappaB) activation by inducing the up-regulation of p21(WAF1) protein expression PMID: 23271730
Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
crystallographic asymmetric unit contains one IL-20-IL-20R1-IL-20R2 complex, corresponding to a solvent content of approximately 54% PMID: 22232181
IL-20 & its receptors were epigenetically regulated through histone post-translational modifications and DNA CpG residue methylation. Also, treatment with recombinant IL-20 resulted in decreased expression of the VEGF family members at the mRNA level. PMID: 21565488
The G allele of the IL-20 -1723C to G polymorphism may contribute to a genetic susceptibility to psoriasis in the Chinese Han population, particularly in patients with psoriasis triggered or exacerbated by an upper respiratory tract infection. PMID: 21109726
in premenopausal obese women, interleukin-20 levels are higher than matched normal weight control women, are associated with body weight and waist-hip ratio, and are reduced by weight loss PMID: 19481430
Essentially identical phenotypes in transgenic mouse models for IL-20, IL-22, and IL-24 suggest that the three related cytokines play a redundant role in keratinocyte differentiation and proliferation. PMID: 20061404
human IL-20 enhanced colony formation by CD34+ multipotential progenitors, buthad no effect on erythroid, granulocyte-macrophage, or megakaryocyte progenitors. PMID: 12855566
IL-19 and IL-20 are synthesized by a distinct population of keratinocytes. PMID: 14675174
Altogether our findings revealed that IL-20 is a negative modulator of COX-2/PGE(2) and inhibits angiogenesis. PMID: 15950941
IL-20 is a pleiotropic cytokine and promotes angiogenesis. PMID: 16511554
IL-20 may influence inflammation through IFN-like effects. These data indicate that IL-20 may be an important effector cytokine in psoriasis, and that its inhibition may represent a potential therapeutic target. PMID: 16645593
Ultraviolet light with photosensitizing agents had little effect or decreased epithelial keratinocyte il-20 levels. PMID: 16709143
IL-20 is a proatherogenic cytokine that contributes to the progression of atherosclerosis. PMID: 16778121
IL-20 protein is expressed in 30 non-neoplastic tissue types and five major cell types: epithelial cells, myoepithelial cells, endothelial cells, macrophages, skeletal muscle cells, and several types of tumor cells. PMID: 16908179
p38 mitogen-activated protein kinase (MAPK) as well as inhibitory kappaB kinase-NF-kappaB signaling pathways are crucial for IL-20 expression in keratinocytes PMID: 17255956
Review. The role of IL-20 in the pathogenesis of inflammatory diseases is reviewed. PMID: 17465720
Activation of toll-like receptors 2 and 3 show keratinocytes in the simultaneous presence of IL-20 and IL-29. PMID: 18281438
results suggest that IL10 and IL20 gene variants influence hepatitis B virus infection outcome PMID: 18479293
IL-20 plays an important role in the pathogenesis of disc herniation. PMID: 18758357
novel effects of IL-20 on mesangial cells and its association with lupus nephritis. PMID: 18771958
IL-20 was responsive to hypoxia in vitro and in the ischemic stroke model and that up-regulation of IL-20 in the ischemic brain may contribute to brain injury PMID: 19342680
Data show that in lesional skin of psoriasis patients, highly elevated IL-20 levels strongly correlated with IL-22, and to a lesser extent, with IL-17A and TNF-alpha. PMID: 19830738

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.