Recombinant Human IL-4 Protein (Mammalian)

Name: Recombinant Human IL-4 Protein (Mammalian)
Brand: Beta LifeScience
SKU: BL-2119NP
Availability: InStock

BL-2119NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant interleukin-4 (il-4) proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed.
Accession	P05112
Synonym	Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
Gene Background	Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.
Molecular Mass	14.97 KDa
Apmol Mass	18 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Subcellular Location	Secreted.
Protein Families	IL-4/IL-13 family
Database References	HGNC: 6014 OMIM: 147780 KEGG: hsa:3565 STRING: 9606.ENSP00000231449 UniGene: Hs.73917
Associated Diseases	Ischemic stroke (ISCHSTR)

Gene Functions References

IL-33 has a role in the pathogenesis of autoimmune hepatitis (AIH) and affects the expression of IL-4, IL-17A, and of hypergammaglobulinemia PMID: 30034292
Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics. PMID: 29236307
Allelic polymorphism C590T of the gene IL-4 is the most possible genetic marker of high predisposition to development of recurrent episodes of acute obstructive bronchitis in children. PMID: 30480406
certain single nucleotide polymorphisms of IL4 gene could predispose individuals to recurrent aphthous stomatitis PMID: 29985726
Germline variants in IL4 gene are associated with prostate cancer. PMID: 29298992
The role of IL-4 in psoriasis. PMID: 28064550
By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
IL-4 polymorphisms might be associated with Kawasaki disease in an Iranian population. PMID: 28036156
association between root resorption and IL4 gene polymorphisms was observed. PMID: 28617966
these results demonstrated that variable number tandem repeat polymorphism in the IL-4 gene is associated with diabetic peripheral neuropathy in type 2 diabetes patients with coexisting cardiovascular disease PMID: 29182400
serum antibodies against HP-NAP represent a state of risk, which is further exacerbated in IL-4 -590 T carriers. PMID: 27677314
Polymorphisms at IL10 (-1082 G>A), IL4 (-589 C>T), CTLA4 (+49A>G), and DAO (+8956 C>G) genes were studied in 55 cases. PMID: 28750137
Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
The results confirmed that the IL-4-590C/T polymorphism is correlated with the onset of RA and that carrying the T-allele can significantly increase the risk of rheumatoid arthritis in the Chinese Han population. PMID: 28975976
the IL-4-590 C>T polymorphism does not influence the development of head and neck cancer. PMID: 29185028
the present study suggests that IL-4 polymorphisms might play a role in susceptibility to inflammatory bowel disease and its major subtypes in the Iranian population PMID: 28872970
IL-4R plays an important role in regulating hepatocellular carcinoma (HCC)cell survival and metastasis, and regulates the activity of the JAK1/STAT6 and JNK/ERK1/2 signaling pathways. We therefore suggest that IL-4/IL-4R may be a new therapeutic target for HCC PMID: 28665449
IL-4 rs2227288 and IL-10 rs1800872 may contribute to an increased risk for virus-induced encephalitis. Through use of direct sequencing, we showed that genotypes of IL-4 rs2227288 and IL-10 rs1800872 may have particular host susceptibility to virus-induced encephalitis. IL-4 rs2227283 and IL-10 rs1800871 have no correlation in with risk of virus-induced encephalitis (both P>0.05) PMID: 28935853
IL-4 and IL-8 genetic polymorphisms determine susceptibility to chronic Aggregatibacter actinomycetemcomitans periodontitis. PMID: 28859277
The data indicate that USP4 interacts with and deubiquitinates IRF4, and also stabilizes IRF4 protein and promotes IRF4 function to facilitate IL-4 expression in Th2 cells, which may be related to the pathological process of rheumatic heart disease. PMID: 28791349
Results show that higher levels of black carbon (BC) were associated with lower methylation of IL4 promoter CpG-48 5 days later and increased FeNO. The magnitude of association between BC exposure and demethylation of IL4 CpG-48 measured 5 days later appeared to be greater among seroatopic children, especially those sensitized to cockroach allergens. PMID: 28588744
Association between the TT-genotype of IL-4 rs2070874 polymorphism and a severe phenotype of viral-induced wheeze further underlines the role IL-4 plays in the inflammation pathway leading to viral respiratory infections. PMID: 28950434
the effects of IL4 gene polymorphisms on cancer risk may vary by cancer type and by ethnicity. PMID: 28656227
these results showed that allergy responses further accelerated the IL-4-induced inhibition of tumor development through the activation of STAT6 pathways. PMID: 28587956
we identified a subgroup of CVID patients with defective IL-4 signaling in T cells, with severe clinical features of inflammation and autoimmunity. PMID: 28476239
The difference between allelic and genotypic frequencies of interleukin-4 (-590C/T) between patients and controls was not significant (p = 0.46). PMID: 29372577
Data suggest that miRNA-340/429, which targeted IL-4, might be a potential approach for cancer treatment. PMID: 27895317
this paper shows that expression of non-secreted IL-4 is associated with histone deacetylase inhibitor-induced cell death, histone acetylation and c-Jun regulation in gamma/delta T-cells PMID: 27556516
Human CCL1 gene is selectively targeted by AhR in M(IL-4) macrophage. IL-4-induced epigenetic modification potentiates AhR-mediated CCL1 expression. PMID: 27888289
Liver IL-4 mRNA is downregulated in patients with pancreatic cancer and cachexia. PMID: 27897439
Low IL4 expression is associated with melanoma. PMID: 26993600
In this review, we discuss the molecular mechanisms driving IL-4 production in Th2 and Tfh cells. PMID: 27072069
In this review, we discuss the structural details of IL-4 and IL-4Ralpha subunit and the structural similarities between IL-4 and IL-13. We also describe detailed chemistry of type-I and type-II receptor complexes and their signaling pathways. Furthermore, we elaborate the strength of type-II hetero dimer signals in response to IL-4 and IL-13. PMID: 27165851
IL-4 and IL-17 modulate the functional activity of phagocytes in the maternal blood, cord blood, and colostrum of diabetic mother. PMID: 29135055
There was no significant difference in serum level of IL-4 between children with MPP (Mycoplasma pneumoniae pneumonia)and those with non-MPP. Among children with MPP, we found similar level of IL-4 regardless of the personal and family history of allergy and asthma or the presence of wheezing. PMID: 28057814
The functional promoter polymorphisms IL4-590C/T and IL6-174G/C, which affect the IL-4 and IL-6 levels in north Indian subjects, were associated with kidney dysfunction and CKD PMID: 27996163
Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. PMID: 29262348
CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
Results from these two large randomized aerobic exercise intervention trials suggest that aerobic exercise does not alter IL-10 or IL-4 in a manner consistent with chronic disease and cancer prevention. PMID: 27485297
IL-4 signaling up-regulates the IL-25 axis in human monocytic cells, and IL-25 may provide autocrine signals in monocytes and macrophages to sustain IL-17Rb expression and predispose to alternative activation. PMID: 28421819
IL4 VNTR B2 allele was only significantly associated with overall adiposity status before adjusting for ethnicity. PMID: 28293435
Our results showed the role of IL4 in promoting breast cancer aggressiveness PMID: 28400477
These data identified the IL-4/CXCL12 loop as a previously unrecognized pathway involved in lymphoid stroma polarization and as a potential therapeutic target in Follicular lymphoma patients. PMID: 28202459
IL-4 genetic variations associated with susceptibility to or protection against chronic periodontitis are directly associated with influencing the response of immune cells to periodontopathogens PMID: 28114408
in HIV/AIDS patients under antiretroviral therapy, IL-4 and IL-10 levels were significantly lower in lipodystrophy vs. non-lipodystrophy PMID: 28189545
autologous CD4(+) T cells that are exposed to EVs from CD40/IL-4-stimulated CLL cells exhibit enhanced migration, immunological synapse signaling, and interactions with tumor cells. PMID: 27118451
IL-4 substantially restores CD79b protein expression, sIgM expression, and BCR signaling. PMID: 27226435
Tandem repeat polymorphisms of IL4 is associated with the severity of chronic periodontitis. PMID: 28053321
Keratinocyte gene expression is critically shaped by IL-4, altering cell fate decisions, which are likely important for the clinical manifestations and pathology of allergic skin disease PMID: 27554818
Inhibition of protein kinase C zeta expression in prostate cancer cells promoted chemotaxis of peripheral macrophages and acquisition of M2 phenotypic features. These results were further supported by the finding that silencing of endogenous protein kinase C zeta promoted the expression of prostate cancer cell-derived interleukin-4 and interleukin-10 PMID: 28631559

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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