Recombinant Human Indian Hedgehog Protein (IHH) Protein (His)

Name: Recombinant Human Indian Hedgehog Protein (IHH) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04397P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) IHH.

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Product Overview

Description	Recombinant Human Indian Hedgehog Protein (IHH) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q14623
Target Symbol	IHH
Synonyms	BDA1; HHG-2; HHG2; IHH; IHH_HUMAN; Indian Hedgehog; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein C-product; Indian hedgehog protein N-product
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	CFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLLFTADNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPGEGVHWYPQLLYRLGRLLLEEGSFHPLGMSGAGS
Expression Range	203-411aa
Protein Length	Full Length of Mature Protein
Mol. Weight	26.6kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
Subcellular Location	[Indian hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Indian hedgehog protein C-product]: Secreted, extracellular space.; Cell membrane.
Protein Families	Hedgehog family
Database References	HGNC: 5956 OMIM: 112500 KEGG: hsa:3549 STRING: 9606.ENSP00000295731 UniGene: Hs.654504
Associated Diseases	Brachydactyly A1 (BDA1); Acrocapitofemoral dysplasia (ACFD)
Tissue Specificity	Expressed in embryonic lung, and in adult kidney and liver.

Gene Functions References

TL1A modulated Rheumatoid arthritis-fibroblast-like synoviocytes migration and Indian hedgehog signaling pathway using TNFR2. PMID: 29748156
Association of pathogenic variants in IHH with short stature with nonspecific skeletal abnormalities and a frequent cause of growth disorder, with a preliminary good response to rhGH. PMID: 29155992
Studies suggest significance of other signaling aside from hedgehog in the pathogenesis of basal cell carcinoma (BCC) of the skin. PMID: 28574612
Gorlin syndrome-derived induced pluripotent stem cells (iPSCs) expressed lower basal levels than control iPSCs of the genes encoding the Hh ligands Indian Hedgehog (IHH) and Sonic Hedgehog (SHH). PMID: 29088246
Hedgehog pathway activation in T-cell acute lymphoblastic leukemia predicts response to SMO and GLI1 inhibitors. PMID: 27694322
CLIC4 and Ihh could serve as biological markers for the progression, metastasis and/or invasiveness of pancreatic ductal adenocarcinoma. PMID: 28205343
The serum levels of IHH and SHH were significantly higher in autistic subjects than those of control subjects. The findings support a correlation between SHH, IHH and BDNF in autistic children, suggesting their pathological role in autism. PMID: 26691363
Our results show for the first time that Indian hedgehog does not cause extracellular matrix degradation in healthy ex vivo cartilage or in the presence of IL-1beta PMID: 26705100
The Annexin a2 Promotes Development in Arthritis through Neovascularization by Amplification Hedgehog Pathway. PMID: 26963384
Studies indicate that the hedgehog (Hh) signaling pathway has become one of the most studied potential therapeutic targets in hematological malignancies. PMID: 24991722
Findings demonstrated that Ihh promotes human cartilage endplate degeneration. PMID: 25958162
endogenously produced IHH is playing a critical role in regulating hBMSC chondrogenesis. PMID: 25519748
Duplication of the upstream IHH regulatory region and a correlation between the phenotype and the implicated regulatory regions in a family with craniosynostosis Philadelphia type. PMID: 25692887
Data show that the Hh (Hedgehog) signalling pathway mediates chemoresistance of BCSCs (breast cancer stem cells) and contributes to the outcome in breast cancer patients. PMID: 26201092
Data suggest that hedgehog (HH) signaling through transcription factors GLI1 and GLI2 could be required for the maintenance of pregnancy. PMID: 25888497
Studies indicate that activation of the hedgehog (Hh) signaling pathway has been observed in several cancer types, including ovarian cancer. PMID: 23303278
Gene transfer of indian hedgehog protein results in chondrogenic induction of mesenchymal stem cells. PMID: 22817660
Data indicate that neutralizing hedgehog antibody MEDI-5304 represents a potent dual hedgehog inhibitor suitable for patients with tumors harboring elevated levels of sonic hedgehog (SHH) or indian hedgehog (IHH). PMID: 24344235
Indian Hedgehog, a critical modulator in osteoarthritis, could be a potential therapeutic target for attenuating cartilage degeneration disease. PMID: 24844414
Increased Ihh is associated with the severity of osteoarthritis cartilage damage. Elevated Ihh content in human knee joint synovial fluid correlates with early cartilage lesions. PMID: 24786088
Data indicate that overexpression of Indian hedgehog (Ihh) signaling promotes abnormal chondrocyte differentiation in enchondral ossification and enhances bone formation in cervical ossification of the posterior longitudinal ligament (OPLL). PMID: 23883825
We further indicated that SHH and IHH were highly expressed in metastatic tumors PMID: 23468532
Studies indicate that upregulation of Hedgehog protein has been found in tumor-associated stroma and associated with vascularization and excessive stromal expansion can limit intratumoral drug delivery. PMID: 23575478
Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner. PMID: 23786654
Data indicate that compounds 7 and 9 (diterpene) showed cytotoxicity against cancer cell lines in which hedgehog (Hh) signaling was aberrantly activated. PMID: 23403897
Data indicate that analysis of public microarray data suggests that PBOV1 activation in tumors could be dependent on the Hedgehog signaling pathway. PMID: 23418531
Results suggest that hypoxia-induced increase of Smo directly contributes to the proliferation of pancreatic ductal adenocarcinoma (PDAC) cells through a hedgehog/Gli1-independent pathway. PMID: 22486854
Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. PMID: 22750747
A missense mutation in IHH gene is observed in an individual affected by brachydactyly type A1. PMID: 22406540
A large duplication involving the IHH locus mimics acrocallosal syndrome PMID: 22234151
Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage. PMID: 22469853
Ihh-induced Nkx3.2 degradation requires Wnt5a, which is capable of triggering Nkx3.2 degradation PMID: 22507129
Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
Studies indicate that IHH is required for normal chondrocyte proliferation during both embryonic and postnatal growth. PMID: 21642379
Nuclear and cytoplasmic endometrial expression of Indian hedgehog is decreased in women with endometriosis. PMID: 21640343
VLDL carries Ihh throughout the body in mammals PMID: 20839884
the observed duplications lead to a misexpression and/or overexpression of IHH and by this affect the complex regulatory signaling network during digit and skull development. PMID: 21167467
This data confirms genetic heterogeneity in Brachydactyly A1 (BDA1) and demonstrates that mutations upstream of IHH can result in BDA1. PMID: 20683927
The temporal increase in endometrial IHH during the secretory phase, and their modulation by CDB-2914 suggests progestin role in endometrial differentiation and implantation. PMID: 20881264
down-regulation of indian hedgehog expression as a result of hypermethylation, may be an early event in colorectal carcinogenesis PMID: 20854074
Methylation analysis revealed that IHH promoter was hypermethylated in colon cancer cell lines. PMID: 19856079
Indian hedgehog protein was detected in cystic tubules within human dysplastic kidneys; these molecules modify severity of this congenital malformation. Hedgehog signaling may be important stimulus for renal cyst formation. PMID: 20007344
describes cloning of human IHH PMID: 7720571
Data show that all of the BDA1 mutations occur in a restricted area of the N-terminal active fragment of the IHH. PMID: 19277064
expression is preserved in the growth plate of human fetuses affected with fibroblast growth factor receptor type 3 activating mutations PMID: 12368206
novel mutation causes brachydactyly type A1 PMID: 12384778
A novel missense mutation in the IHH gene mapping to chromosome 5 has been identified identified in all patients of a brachydactyly type A1 family. PMID: 12525541
Homozygous mutations in this gene cause acrocapitofemoral dysplasia, an autosomal recessive disorder with cone-shaped epiphyses in hands and hips. PMID: 12632327
model of the interactions between beta-catenin and hedgehog signaling in the epidermis in which SHH promotes proliferation of progenitors of the hair lineages whereas IHH stimulates proliferation of sebocyte precursors PMID: 12917489
a wide range of digestive tract tumours, including most of those originating in the oesophagus, stomach, biliary tract and pancreas, but not in the colon, display increased Hh pathway activity, which is suppressible by cyclopamine, a Hh pathway antagonist PMID: 14520411

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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