Recombinant Human Integrin Alpha-5 (ITGA5) Protein (His), Active

Name: Recombinant Human Integrin Alpha-5 (ITGA5) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05695P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Description	Recombinant Human Integrin Alpha-5 (ITGA5) Protein (His), Active is produced by our Mammalian cell expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	The ED50 as determined by its ability to bind Human NovoNectin in functional ELISA is less than 50 ug/ml.
Uniprotkb	P08648
Target Symbol	ITGA5
Synonyms	CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; Fibronectin receptor; alpha subunit; FNRA; Integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-5 light chain; Integrin alpha-F; Integrin; alpha 5 (fibronectin receptor; alpha polypeptide); ITA5_HUMAN; Itga5; Very late activation protein 5; alpha subunit; VLA-5; VLA5; VLA5A
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	C-6His
Complete Sequence	FNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY
Expression Range	42-995aa
Protein Length	Partial
Mol. Weight	105.11 kDa
Research Area	Signal Transduction
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.; (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.; (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Subcellular Location	Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Cell surface.
Protein Families	Integrin alpha chain family
Database References	HGNC: 6141 OMIM: 135620 KEGG: hsa:3678 STRING: 9606.ENSP00000293379 UniGene: Hs.505654

Gene Functions References

The results showed that in cervical cancer cells Rac1 activation by hypoxia could stimulate invasion and migration, and this process was mediated by integrin a5b3-facilitated FAK and PI3K phosphorylation. PMID: 29358562
A specific component of the gastric refluxate, deoxycholic acid, affects the epithelial barrier through modulation of integrin alpha V expression, providing a novel mechanism for bile acid-mediated erosion of esophageal squamous epithelium and promotion of Barrett's Esophagus. PMID: 28941013
first PET/CT scans of head and neck squamous cell carcinoma (HNSCC) and NSCLC patients displayed SFITGv6 accumulation specifically in tumors, but not in inflammatory lesions.Thus, SFITGv6 represents a novel powerful tracer for imaging and possibly for endoradiotherapy of ITGa5b6-positive carcinoma PMID: 28468949
Endothelial cell-derived matrix promotes the metabolic functional maturation of hepatocyte via integrin-Src signaling. PMID: 28470937
integrin alpha5 might be involved in Aggregatibacter actinomycetemcomitans Y4 invasion into gingival epithelial cells, and the resulting signal transduction cascade reduces cell adhesion by decreasing the expression of integrins, while the TLR2/4 signaling cascade regulates IL-8 expression. PMID: 28593565
Fibronectin fragments (FNFr) function as matrikines driving the chemotactic affinity of prostate cancer cells via the alpha5beta1 integrin. PMID: 27715399
Gene function analyses in in-vitro cultured adipose tissue stem cells (ASCs) indicated differential functions of integrin-alpha-5 (ITGA5) and integrin-alpha-V (ITGAV). PMID: 27363302
Hypoxia induces the expression of ITGA5 in breast cancer.ITGA5 plays role in the breast cancer metastasis. PMID: 28213554
miR-330-5p could suppress proliferation and invasion of glioblastoma cells through targeting ITGA5. PMID: 28336765
Inhibiting Fgf-R partly reversed alphavbeta3 integrin activity in Mll-Ell+ progenitor cells. PMID: 27340869
Thus, Aspergillus fumigatus CalA is an invasin that interacts with integrin alpha5beta1 on host cells, induces endocytosis and enhances virulence. PMID: 27841851
Data map the network of interactions between host-cell alpha5beta1 integrin and the cagT4SS proteins. PMID: 29055076
Data indicate integrinalphavbeta3 is a tumor target on breast cancer bone metastases. PMID: 28855208
we compared the characteristics of tumors with and without wild-type (wt) cANGPTL4 and tumors with cANGPTL4 bearing the T266M mutation (T266M cANGPTL4). We found that T266M cANGPTL4 bound to integrin alpha5beta1 with a reduced affinity compared to wt, leading to weaker activation of downstream signaling molecules. PMID: 28641978
Osteoprotegerin facilitates pulmonary arterial hypertension pathogenesis by regulating pulmonary arterial smooth muscle cell proliferation via integrin alphavbeta3/FAK/AKT signaling pathway. PMID: 28077433
alpha5beta1 integrin can stimulate drug resistance in cancer cells via a mechanism based on inhibition of protein kinase Erk. PMID: 28988530
We measured the effect of recombinant Gal-3 added exogenously on the lateral mobility of the alpha5b1 integrin on HeLa cells. Using single-particle tracking (SPT) we detected increased lateral mobility of the integrin in the presence of Gal-3, while its truncated C-terminal domain (Gal-3C) showed only minor reductions in lateral mobility. PMID: 29016609
pStat-3 and integrin alphavbeta6 were indicators of tumor's progression and poor prognosis of patients with gallbladder cancer PMID: 28061445
Eps8/Abi1/Sos1 tricomplex acts as a key molecular switch altering the balance between Rac1 and Rho activation; its presence or absence in pancreatic ductal adenocarcinoma cells modulates alphavbeta6-dependent functions, resulting in a pro-migratory (Rac1-dependent) or a pro-TGF-beta1 activation (Rho-dependent) functional phenotype. PMID: 28608476
Our study is the first to report that treatment with a RARgamma specific agonist augments cellular adhesion to alpha5beta1 integrin substrates, increases cell surface levels of the beta1 integrin subunit, and dampens cellular proliferation in a time and concentration dependent manner in a human erythroleukemia cell line PMID: 28552962
Findings indicate integrin inhibition as a promising strategy to block both aryl hydrocarbon receptor (AhR) and transforming growth factor beta (TGF-beta)-controlled features of malignancy in glioblastoma. PMID: 26500056
Analysis of alphavbeta3 and alpha5beta1 integrin clusters indicates that fibrillar adhesions are more prominent in cells adhering to alpha5beta1 ligand, while clusters are mostly localized at the cell margins in cells adhering to alphavbeta3 ligand. PMID: 27003228
Suggest an adhesion-dependent mechanism of RUNX2 for the osteotropism and bone colonization of breast cancer cells and implicate RUNX2 and integrin alpha5 as potential molecular markers for the prediction of bone metastasis. PMID: 27317874
cells on fibronectin suppressed cAMP via enhanced phosphodiesterase (PDE) activity, through direct binding of integrin alpha5 to phosphodiesterase-4D5 PMID: 27595237
N-Glycosylation on integrin alpha5 is required for alpha5beta1-mediated cell migration. PMID: 28167607
Data show that high mobility group nucleosomal binding domain 2 (HMGN2) knockdown induced the increased expression of alpha5beta1 integrin on cell membranes, which resulted in a significant increase in Klebsiella pneumoniae internalization. PMID: 27460641
ITGA5 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
ectopic expression of miR-17 in ovarian cancer cells resulted in repressed ILK phosphorylation as well as decreased production of active matrix metalloproteinase-2 (MMP-2). Our results indicated that miR-17 hampered ovarian cancer peritoneal propagation by targeting integrin a5 and b1. PMID: 27499367
These results suggest that downregulation of miR-330-5p expression may affect Colorectal cancerdevelopment via modulation of ITGA5 expression. PMID: 27633518
In colorectal cancer cells, miR-130b directly targeted the 3' untranslated region (3'UTR) region of integrin alpha5 gene, which encodes a key molecule involved in cell motility. TGF-beta1 acted through miR-130b to promote integrin alpha5 expression, resulting in the enhanced migration of CRC cells. PMID: 26873488
Suggest that in gliomas, integrin alpha5-mediated dissemination from the tumor mass overrides integrin alpha5-mediated tumor cell cohesion. PMID: 27063097
upregulation of miR-31 targeting ITGA5 may suppress tumor cell invasion and metastasis by indirectly regulating PI3K/AKT signaling pathway in human SGC7901 GC cells PMID: 26729197
The aim of the present study was to characterize the roles of ITGalpha5 and ITGbeta1 in the proliferation and migration of vascular smooth muscle cells. PMID: 26648324
ITGA5 plays an important role in maintaining and regulating the normal migration capacity of dental pulp stem cells. PMID: 26823759
IGFBP-2 enhances endothelial progenitor cell adhesion to HUVECs via Integrin alpha5beta1. The RGD motif in the IGFBP-2 C-domain is required for interaction with integrin alpha5beta1. PMID: 26076738
The present study has revealed an integrin- and membrane lipid raft-dependent mechanotransduction mechanism by which atheroprone flow causes endothelial dysfunction. PMID: 26733684
Data show that the fold changes of very late antigen 5 VLA-5 (integrin alpha-5 beta-1) in CD14+ monocytes were higher than those observed for THP-1 cells. PMID: 26159767
integrin alpha5 might play important roles in the progression of esophageal squamous cell carcinoma PMID: 26772401
Data suggest that reduced ability of AnxA6-expressing cells to migrate is associated with decreased cell surface expression of integrins alphaVbeta3 and alpha5beta1. PMID: 26578516
Data suggest that the binding, but not the internalization of photoreceptor outer segments (POS) confers protective effects on retinal pigment epithelium (RPE) cells through the alphavbeta5 integrin/focal adhesion kinase (FAK)/PGC-1alpha pathway. PMID: 26244551
alpha5beta1 and alphavbeta3 each mediate actin cytoskeletal remodeling in response to stiffening or cyclic stretching of the extracellular matrix. PMID: 25663698
Integrin alpha5beta1 was expressed on ligamentum flavum fibroblasts. PMID: 25735609
Data suggest that Entamoeba histolytica-macrophage contact triggers recruitment of alpha5beta1 integrin (ITGA5/ITGB1) and NLRP3 into intercellular junction, where ITGA5/ITGB1 undergoes activation by cysteine protease on parasite surface. PMID: 25955828
Data show that thyroid hormones induce mitogen-activated protein kinase (MAPK) via integrin alphavbeta3 in bone marrow-derived cells from multiple myeloma (MM). PMID: 25058375
Integrin alphanubeta6-mediated MMP-9 secretion facilitates pericellular matrix degradation at high cell density, promoting invasive growth of colonic neoplasms. PMID: 26139991
Data show the molecular model of the complex of activated protein C (APC) with alphaVbeta3 integrin obtained by protein-protein docking approach. PMID: 24251463
Data indicate that alpha5 integrin (ITGA5) is a functional target of miroRNA miR-26a-induced anoikis in hepatocellular carcinoma (HCC) cells. PMID: 25537511
Collagens considerably suppressed alpha5 gene expression in CECs, suggesting that during wound PMID: 26431475
Suggest alpha5beta1/Arp2/Arp3/FHOD3 pathway reprograms the actin cytoskeleton to promote invasive migration and local invasion in vivo. PMID: 26370503
In the presence of both 9-cis-retinoic acid and troglitazone, cell surface alpha5 subunit expression was restored to levels comparable to vehicle treatment alone. PMID: 24671180

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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