Recombinant Human Interleukin-12 Subunit Alpha Protein (IL12A), Active

Name: Recombinant Human Interleukin-12 Subunit Alpha Protein (IL12A), Active
Brand: Beta LifeScience
SKU: BLC-05394P
Availability: InStock

Greater than 97% as determined by SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-12 Subunit Alpha Protein (IL12A), Active is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard
Uniprotkb	P29459
Target Symbol	IL12A
Synonyms	CLMF; CLMF p35; CLMF p40; CLMF1; CLMF2; Cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 2; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12A; IL 2 subunit p40; IL-12 subunit p35; IL-12A; IL-12B; IL12; IL12, p35 subunit; IL12, subunit p40; IL12A; IL12A_HUMAN; IL12B; IL35 subunit; Interleukin 12 alpha chain; Interleukin 12 beta chain; Interleukin 12 subunit beta; Interleukin 12, p40; Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); Interleukin 12B; Interleukin-12 subunit alpha; Natural killer cell stimulatory factor 1, 35 kD subunit; natural killer cell stimulatory factor 2; Natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	Tag-Free
Complete Sequence	p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Expression Range	23-219aa&23-328aa
Protein Length	Full Length of Mature Protein
Mol. Weight	57kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	0.2 μm filtered PBS, pH 7.4, lyophilized
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Target Details

Target Function	Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subcellular Location	Secreted.
Protein Families	IL-6 superfamily
Database References	HGNC: 5969 OMIM: 161560 KEGG: hsa:3592 STRING: 9606.ENSP00000303231 UniGene: Hs.673

Gene Functions References

These results suggest that IL-12A rs568408 may contribute to the etiology and symptoms severity of asthma, indicating its usefulness as a predictive and diagnostic biomarker of asthma. PMID: 28600552
Study provides evidence that the AA genotype of IL-12A rs568408 is associated with an increased lung cancer risk among Taiwanese, especially those with smoking habits. PMID: 30396953
IL-35 may have a role in renal involvement in lupus nephritis patients PMID: 29576585
The IL-35 levels in CagA(+) H. pylori-infected participants from peptic ulcer and H. pylori-infected asymptomatic groups were lower than individuals infected with CagA(-) strains. PMID: 29938865
these data demonstrate the expression of IL35 in oral lichen planus lesions PMID: 29633015
IL-12 receptor signalling in keratinocytes initiates a protective transcriptional programme that limits skin inflammation. PMID: 27892456
The present study investigated the relationship of IL35 in patients with immunorelated hemocytopenia (IRH). Serum level of IL35 was decreased in untreated patients with IRH compared with remission patients (P<0.01) and was significantly associated with clinical indexes. PMID: 29257310
IL-35 may be involved in the pathogenesis of Primary biliary cirrhosis. PMID: 29445068
The overall results showed that cancer risk was increased by IL-12A rs568408 (GG versus GA + AA: P = 0.004; G versus A: P = 0.005) and IL-12B rs3212227 (AA versus AC + CC: P = 0.004; CC versus AA + AC: P = 0.03; A versus C: P = 0.007) polymorphisms. However, we failed to detect any significant associations between the IL-12A rs2243115 polymorphism and cancer risk in either the overall or the subgroup analyses. PMID: 28408727
IL-35 is a relatively newly discovered member of the IL-12 family which are unique in structure as they are dimer formed by two subunits; recent findings have shown abnormal expression of IL-35 in inflammatory autoimmune diseases and functional analysis suggested that IL-35 is critical in the onset and development of these diseases. [Review] PMID: 29729445
The results suggest IL12A genotype predict higher risk of death and progression, respectively, in NSCLC patients. PMID: 28807247
IL-35 expression was significantly increased in patients with chronic hepatitis C and was positively correlated with the levels of viral RNA PMID: 28644966
Pancreatic ductal carcinoma cells produce IL35 to recruit monocytes via CCL5 and induce macrophage to promote angiogenesis via expression of CXCL1 and CXCL8. PMID: 28989066
Polymorphisms of IFNL3 rs8099917 and IL12A rs568408 contribute to survival of HD patients, but not as independent factors. PMID: 28525983
The anti-EGFR viroplexes with IL-12 and salmosin genes exhibited the most effective therapeutic efficacy in a mouse tumor model, especially when combined with doxorubicin. PMID: 27191929
baseline serum IL-12 levels were significantly higher in those pediatric hematopoietic stem cell transplantation recipients who did not develop acute graft versus host disease PMID: 27751764
Our data indicated that decreased expression of IL-35 in tumor tissues might contribute to the progression of hepatocellular carcinoma PMID: 27329841
circulating mir-21 and expression of the IL-12p35 gene were observed significantly downregulated in current smokers with CAD. PMID: 27765464
Simultaneous elevated circulating levels of IL-18 and IL-12 increased the event rate after 2 years in Coronary Artery Disease patients, independent of hyperglycemia PMID: 27058587
The study revealed that post-therapeutic recovery of circulating IL-35 concentration might be an independent predictor for effective response to IST in pediatric AA. PMID: 28211781
Epstein Barr Virus miRNAs collectively and specifically suppress release of proinflammatory cytokines such as IL-12, repress differentiation of naive CD4(+) T cells to Th1 cells. PMID: 27621419
assembly-induced folding is key in IL-12 family biogenesis and secretion; the identification of essential disulfide bonds that underlie this process lays the basis for a simplified yet functional IL-12 cytokine PMID: 28325840
The plasma levels of interleukin-35 were significantly higher in the hepatocellular carcinoma patients than the controls. PMID: 27699510
Importantly, treatment of NK cells from HIV-infected patients with IL-12 reversed the multiple defects in binding, granule polarization, perforin content, and perforin release and restored anticryptococcal activity. PMID: 27555306
The results of this case-control study suggest that IL-12A, IL-12B, IL12RB1, IL12RB2 and IL23R make no genetic contribution to the susceptibility of Takayasu arteritis in Chinese populations PMID: 26987707
this study introduces IL-35 as a new treatment for pemphigus PMID: 27855302
suberoylanilide hydroxamic acid significantly inhibited IL-12p40 and IL-23p19 mRNA synthesis and did not change IL-12p35 mRNA transcription. PMID: 27240992
this study shows that IL-12A gene polymorphism contributes to the risk of hepatocellular carcinoma on top of chronic hepatitis C virus infection in Egyptian patients PMID: 27819525
The results suggest that hepatocellular carcinoma (HCC). cell apoptosis was suppressed at least partially through HBx-induced miR-21 by targeting IL-12. PMID: 27571873
These data indicate that increased serum IL-35 could act as a disease activity marker and as a risk factor for esophageal involvement in idiopathic inflammatory myopathies. PMID: 27502600
The results of Expression Quantitative Trait Loci analysis showed that the risk alleles of rs4679868 and rs6441286 were significantly associated with decreased expression of IL12A in lymphoblastoid cell lines derived from individuals of Chinese Han ancestry. These results demonstrated that IL12A is associated with Primary Biliary Cirrhosis in the Chinese Han population. PMID: 27175695
The highest gene transfer ability was achieved by the DTPA-conjugated PEI at the conjugation degree of 0.1 % where the level of IL-12 production increased up to twofold compared with that of the unmodified PEI PMID: 26801817
TLR3 is required to induce full IL-12p70 secretion. PMID: 26956584
Interleukin-12 A rs17810546 was positively associated with celiac disease. PMID: 27043536
Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) levels were significantly elevated in the patients with influenza infection. PMID: 26844658
the functional single nucleotide polymorphism IL-12A rs2243115 GG genotype may increase the risk of rheumatoid arthritis in rheumatoid factor negative patients PMID: 26375522
Low IL12 expression levels are associated with Acute Leukemia. PMID: 26514528
Study indicated that the rs568408 variation in IL-12A gene could influence disease susceptibility, and was associated with the severity of oral lichen planus in our East Chinese cohort. PMID: 25258182
These results for the first time demonstrate a role of Cdk5/p35 in the regulation of cell cycle progression modulated by TGF-beta1. PMID: 26966064
IL-35 was elevated in bone marrow of adult AML patients and this increase was correlated with the clinical stages of malignancy, suggesting that IL-35 is involved in pathogenesis of AML. PMID: 26431888
p41 fragment is also shown to reduce the secretion of interleukin-12 (IL-12/p70) during the subsequent maturation of treated dendritic cells. PMID: 26960148
IL-35 plays a protective role in the pathogenesis of rheumatoid arthritis PMID: 26370008
Findings demonstrate that IL-12 is increased significantly in chronic hepatitis B patients and that viral protein HBx induces IL-12 expression in hepatocytes through the activation of the PI3K/Akt pathway. PMID: 26062743
Serum levels of IL-35 increased in normal pregnancy and decreased in recurrent spontaneous abortion. PMID: 26042836
IL-35 Is Involved in the Pathogenesis of Guillain-Barre Syndrome Through Its Influence on the Function of CD4+ T Cells PMID: 26225474
Elevated circulating IL-35, particularly at early phase, its decrease after treatment initiation, and a positive association between synovial fluid IL-35 and disease activity support an involvement of IL-35 in the pathogenesis of RA. PMID: 26204444
Gene-gene interaction analysis showed that subjects carrying the IL-12A rs568408AG/AA and IL-12B rs3212227AA genotypes had a 2.40-fold increased risk of CRC (OR = 2.40; 95 % CI, 1.14-5.07) PMID: 26104769
the distribution of interleukin (IL)-12 (IL12; 1188A/C), IL17 (A7488G) and IL-23 receptor (IL23R; +2199A/C) gene polymorphisms in patients with alopecia areata, was investigated. PMID: 26017027
Genome-wide association study in an admixed case series reveals IL12A as a new candidate in Behcet disease PMID: 25799145
These results might indicate an important new role for IL-35 as an autocrine growth factor in pancreatic cancer growth. PMID: 25073578

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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