Recombinant Human MCP-4 / CCL13 Protein

Name: Recombinant Human MCP-4 / CCL13 Protein
Brand: Beta LifeScience
SKU: BL-1219SG
Availability: InStock

Collections: Chemokines and receptors, Cytokines and growth factors, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	Q99616
Synonym	Monocyte chemotactic protein 4, CCL13, NCC-1, C-C motif chemokine 13, Small-inducible cytokine A13, MCP4, NCC1, SCYA13,Recombinant Human Monocyte Chemoattractant Protein-4 (CCL13)
Background	Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is member of a distinct, structurally-related subclass of CC chemokines. MCP-4/CCL13 is a major chemoattractants for eosinophils, basophils, monocytes, and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell. Expression of MCP-4/CCL13 mRNA and protein is greater in the sputum, epithelium, submucosal inflammatory cells, and bronchoalveolar lavage fluid of asthmatics than in healthy individuals. Involvement of MCPs has also been demonstrated in renal inflammation and atopic dermatitis. The expression of this protein is also correlated with enhanced inflammatory immune responses during immunotherapy. The recombinant construct corresponds to the -€œshort-chain-€ of CCL13 spanning amino acids 24 - 98.
Description	Recombinant Human MCP-4 (CCL13) was produced in E. coli. This protein is purified with our unique purification methods.
Source	E.coli
Molecular Weight	9.0 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human MCP-4 / CCL13 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	HGNC: 10611 OMIM: 601391 KEGG: hsa:6357 STRING: 9606.ENSP00000225844 UniGene: Hs.414629
Tissue Specificity	Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Gene Functions References

CCL13 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
CCL13 levels in serum and synovial fluid may serve as a biomarker for the progression of osteoarthritis. PMID: 25654294
CCL13 is an antimicrobial protein with bacteriocidal activity against E. coli. PMID: 12949249
Data suggest that CCL13 binds to several chemokine receptors (CCR1, CCR2, and CCR3), allowing CCL13 to elicit different effects on target cells of immune system. CCL13 is involved in pathology of chronic inflammatory/autoimmune diseases. [REVIEW] PMID: 23846739
MCP-4 and hsCRP may be the markers linking chronic inflammation in obesity and periodontal disease. PMID: 23375121
E(2) has adverse effects on the pathogenesis of RA as a result of unregulated cell death, increased TNF-alpha-induced MMP-3 production, and CCL13 overproduction, subsequently resulting in the disease progression of RA. PMID: 22393877
Findings suggest that the pro-atherogenic effects of CCR2 may not be restricted to interaction with MCP-1, but could also involve activation by MCP-4, being an inflammatory link between platelet and monocyte activation. PMID: 20139115
Data suggest that the monocyte chemoattractant protein-4 core promoter YY-1 binding motif is functional, and modulates the transcriptional regulation of the MCP-4 gene. PMID: 12805085
The study was undertaken to understand the relationship between the functional proteomics of receptor-Ck and developmental stages of human atherosclerotic aortic wall. PMID: 14560935
MCP-4 may be a critical molecule that links obesity and chronic inflammation. PMID: 16855189
The Th2 cytokine IL-4 preferentially stimulated MCP-4 expression. PMID: 17541284
interferon-gamma with IL-1beta/TNF-alpha activates the production of CCL13 from chondrocytes in RA joints; secreted CCL13 enhances fibroblast-like synoviocyte proliferation by activating the extracellular signal-regulated MAP kinases cascade PMID: 17824960
TLR2, 3, 4 and 5 ligands, but not TLR7/8 or 9 ligands, synergized with IL-4 to induce the production of MCP-4 in nasal polyp fibroblasts. PMID: 17851891
CCL13 and HSPC159 mRNA expressions in PBMC are up-regulated specifically in MCNS patients during the nephrosis phase. PMID: 18219197
Oncostatin M can strongly up-regulate the expression of CCL13, a chemokine recently identified in the synovial fluid of patients with rheumatoid arthritis. PMID: 19565514

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.