Recombinant Human MMP-3 Protein (C-6His)

Name: Recombinant Human MMP-3 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1328NP
Availability: InStock

BL-1328NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Enzymes, Protease, Recombinant proteins

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Product Overview

Description	Recombinant Human Matrix Metalloproteinase-3 is produced by our Mammalian expression system and the target gene encoding Tyr18-Cys477 is expressed with a 6His tag at the C-terminus.The proenzyme needs to be activated by Chymotrypsin for an activated form.
Accession	AAA36321.1
Synonym	Stromelysin-1; SL-1; Matrix metalloproteinase-3; MMP-3; Transin-1; MMP3; STMY1
Gene Background	MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling.[3] The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
Molecular Mass	53.26 KDa
Apmol Mass	50-65 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Peptidase M10A family
Database References	HGNC: 7173 OMIM: 185250 KEGG: hsa:4314 STRING: 9606.ENSP00000299855 UniGene: Hs.375129
Associated Diseases	Coronary heart disease 6 (CHDS6)

Gene Functions References

BMAL1 Deficiency Contributes to Mandibular Dysplasia by Upregulating MMP3. PMID: 29276151
rs3025058 in MMP3 and rs2276109 in MMP12 might not contribute to the risk of developing rheumatic heart disease in a Han population in Southern China. PMID: 29458338
study to explore the relationship between 2 polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration; a significant association was found between the MMP-3 polymorphism and disc degeneration; the homozygote CC was associated with an increased risk of disc degeneration compared with the AA genotype PMID: 28497435
Higher serum MMP-3 levels in knee osteoarthritis reflect disease "generalization". PMID: 29164307
Report a positive correlation between glomerular filtration rate and MMP-3 activity in diabetic patients. PMID: 29421776
The maternal and fetal MMP3 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668
Preliminary studies indicate that baseline MMP3 and TIMP3 concentrations are associated with patient survival and disease-free time PMID: 29304854
MMP-3 (Lys45Glu) polymorphisms associate with obesity risk and its severity. PMID: 29317790
Data suggest that serum matrix metalloproteinase-3 (MMP-3) and the 7-joint ultrasound score (US7) scores could both effectively reflect disease activity and therapeutic responses in patients with moderate to severe rheumatoid arthritis (RA). PMID: 29141665
results demonstrated that measurement of MMP-3 could become a marker of disease activity in rheumatoid arthritis patients PMID: 28461705
The results indicated that the 5A allele of the MMP3 gene-promoter region could be associated with oral submucous fibrosis risk factor, but not oral squamous cell carcinoma, in an Indian population PMID: 27389714
C/EBPbeta upregulation promoted tumor cell invasion in an MMP3-dependent manner in vitro and was associated with metastatic status in colorectal cancer PMID: 29172775
MMP3 (and MMP-1 and MMP-2) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
A significant increase in the frequency of the MMP3-1171dupA (rs3025058) polymorphism genotype among patients with unstable angina was detected. PMID: 29044936
No interaction between MMP-3 5A/6A polymorphism and the risk of recurrence in total IS patients was found. The variant 5A/6A+5A/5A genotype and the 5A allele were significantly associated with a high risk of recurrence for large-artery atherosclerosis (LAA) (multivariate-adjusted, P=0.002, 0.001, respectively), but not for small-artery occlusion and cardioembolism. PMID: 28843428
The 5A allele of the MMP3-gene promoter polymorphism is a potential risk factor in the poor outcome of hemodialysis patients. PMID: 28781337
Positive MMP-3 was not significantly related to osteoporosis or severe hand osteoarthritis. PMID: 28365812
In patients with ankylosing spondylitis, serum MMP3 level was found to have a positive correlation with the MRI score of sacroiliac joint and c-reactive protein. PMID: 28432524
Suppression of active MMP3, but not total circulating MMP3 was associated with treatment response in rheumatoid arthritis patients treated with tocilizumab. PMID: 28850021
These results, for the first time, demonstrate that P15 binding to cell surface vimentin inhibits the tumor cell invasion and is associated with reduced MMP3 expression. Thus, suggesting that P15 has potential as an anti-metastatic therapy in pancreatic cancer. PMID: 28396463
A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
studies establish a molecular imaging reporter for diagnosing early-stage EOC. Additional studies are required to confirm the early-stage activity of MMP-3 in EOC and its diagnostic and prognostic significance PMID: 29390034
among Chinese males, MMP-3 rs650108 and MMP-8 rs2012390 decrease alcohol-induced ONFH risk and MMP-8 rs11225394 increases it. PMID: 28445942
Report an association between the MMP-3 rs3025058 and subclinical markers of carotid and coronary atherosclerosis at the time of recruitment. MMP-3 rs3025058 on affects carotid artery disease progression in the 3.8-year follow-up in patients with T2DM. PMID: 28521653
promoter polymorphism rs3025058 associated with adolescent idiopathic scoliosis but not with gender bias PMID: 27911281
mRNA levels of HSP family members (HSP70B', HSP72, HSP40/DNAJ, and HSP20/CRYAB) are upregulated by the intracellular MMP3 overload. PMID: 27206651
These observations suggest a crucial role for cancer-associated fibroblasts and fibroblast growth factor-1/fibroblast growth factor receptor 4 signaling in the progression of ovarian cancer. the expression level of Snail1 and MMP3 was reduced, while the expression level of E-cadherin increased PMID: 28718374
MMP3 overexpression is associated with Melanoma Tumor Growth and Metastasis. PMID: 27013197
Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. PMID: 27391467
Three previously investigated MMP3 variants (rs679620, rs591058 and rs650108) in addition to the functional promoter variant (rs3025058) were genotyped in 195 Australian control participants and 79 Australian individuals with chronic Achilles tendinopathy. PMID: 27211292
MMP-3 expression is deregulated in osteosarcomas and this potentially contributes to metastasis and might be a promising marker for the prognosis and therapy of metastatic osteosarcoma. PMID: 27837634
These results indicate that periodic induction, via use of an eye drop, of AAV-mediated secretion of MMP-3 into aqueous humour could have therapeutic potential for those cases of glaucoma that are sub-optimally responsive to conventional pressure-reducing medications. PMID: 28158775
In summary, our study demonstrates that IL-7/IL-7R axis promotes the invasion and migration of prostate cancer cells, through activation of AKT/NF-kappaB pathway and upregulation of MMP-3 and MMP-7 expression PMID: 27611862
Study showed that serum MMP-3 levels are decreased in patients with epilepsy, which is relevant for acute stroke. A specific pathogenic effect of MMP-3 in epilepsy appears unlikely. This study has demonstrated that the newly developed Luminex assays are useful in quantifying MMP-3 levels in human serum. PMID: 27423606
Serum baseline levels of MMP-3 are strong prognostic markers of disease activity, and act well as an early predictor of progressive joint damage in recent-onset rheumatoid arthritis. PMID: 25292349
MMP-3 positivity has a strong relationship with meningiomas having an aggressive character; MMP-3 may be used as a proliferation marker for biological behaviour, recurrence rate and prognosis of meningiomas PMID: 27438616
5A5A genotype of MMP-3-1171 5A/6A gene polymorphism was associated with adolescent idiopathic scoliosis, especially in Caucasian population. (Meta-analysis) PMID: 27659327
PAF stimulation dose-dependently promoted the invasion, migration and growth of prostate cancer cells in vitro, while knockdow our findings demonstrate that PAFR can activate ERK1/2 pathway, and subsequently increase MMP-3 expression and decrease E-cadherin expression PMID: 27176648
5A/6A polymorphism of matrix metalloproteinase-3 may contribute to recurrent atherosclerotic ischemic stroke in Chinese. PMID: 26314579
We concluded that overexpression of MMP-3 and uPA, altogether with diminished expression of PAI-1 from metastatic tumors, might be a crucial step towards metastasis in ductal breast cancer. PMID: 27975070
NFAT1 silencing could suppress cell migration and invasion through MMP-3. PMID: 28024290
Specific gene polymorphisms are known to be associated with a different arterial physiology in the younger generation. The present study found that young Russians with the matrix metalloproteinase 3 6A/6A and gamma-glutamyltransferase 1AA genotypes have lower levels of the cardio-ankle vascular index - a recent measure of arterial stiffness. PMID: 27161754
MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. MMP-3 expression and the histological and radiological scores were positively correlated. PMID: 27706715
High MMP3 expression is associated with Ovarian Tumors. PMID: 27268637
MMP3 gene -1171 5A/6A polymorphism and upregulated protein expression may be associated with deep vein thrombosis risk in Chinese Han population. PMID: 27177702
data demonstrated that the MMP3 rs650108 variant was significantly associated with increased frozen shoulder susceptibility in a Chinese Han population. PMID: 27051023
MMP3 is released from the synovial membrane under inflammatory conditions and may serve as biological markers for inflammatory osteoarthritis. PMID: 26863055
This study showed an increased frequency of heterozygotes for stromelysin-1 rs3025058 and thought to be involved in the etiology of Gastric cancer PMID: 26853425
These results indicate an essential role for MAPKs in the induction of MMP-3 in synovial sarcoma cells, through AP-1 activation. PMID: 26850593
A low level of MMP-3 is an excellent positive predictor for polymyalgia rheumatica with giant cell arteritis. PMID: 26156043

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