Recombinant Human/Mouse/Rat Irisin Protein (N-6His)

Name: Recombinant Human/Mouse/Rat Irisin Protein (N-6His)
Brand: Beta LifeScience
SKU: BL-2198NP
Availability: InStock

BL-2198NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Cytokines, Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human/Mouse/Rat Fibronectin Type III Domain-containing Protein 5 is produced by our Mammalian expression system and the target gene encoding Asp32-Glu143 is expressed with a 6His tag at the N-terminus.
Accession	Q8NAU1
Synonym	Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin; FNDC5
Gene Background	Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Molecular Mass	13.4 KDa
Apmol Mass	18-28 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein.; [Irisin]: Secreted.
Database References	HGNC: 20240 OMIM: 611906 KEGG: hsa:252995 STRING: 9606.ENSP00000362570 UniGene: Hs.524234
Tissue Specificity	Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen.

Gene Functions References

Lower levels of irisin were associated with 1.6 times increased cardiovascular disease risk. PMID: 29673927
There is a decrease in serum irisin level in type 2 diabetic patients with even more significant reduction in patients with diabetic nephropathy. PMID: 28875825
FNDC5 mRNA expression in skeletal muscle and circulating irisin concentration relatively to exercise mode, intensity, frequency and duration and the characteristics of the sample used PMID: 29127759
Serum concentrations of irisin are elevated in post-myocardial infarction patients with increased risk for adverse cardiovascular events. PMID: 29657036
Acute high-intensity interval exercise decreased irisin levels and increased myostatin levels. PMID: 29558345
serum levels were significantly lower in pre-eclampsia than normotensive pregnancies PMID: 29430974
Glucocorticoid receptor positively regulates transcription of FNDC5 in the liver. PMID: 28240298
This study finds that soccer matches performed different workout times have strong stimulatory effects on irisin levels in all subjects but nesfatin-1 response varied among the subjects and it did not change significantly in afternoon match. PMID: 30084805
Results show that Irisin concentration in umbilical cord blood was found to be associated with preterm birth (PTB). On the other hand, no differences in maternal blood irisin levels between mothers with preterm and term deliveries were established. PMID: 29997715
Irisin concentrations do not predict risk of developing diabetes prospectively PMID: 29108900
serum irisin levels were significantly lower in patients with psoriasis, and associated with serum lipid levels and disease activity; these results can be interpreted that irisin is involved in the disease pathogenesis of patients with psoriasis in relation to metabolic dysregulation PMID: 27652568
This study show the serum irisin levels had significant positive correlation with seizure severity Chalfont score and the duration of epilepsy. PMID: 29860632
clinical evidence of the association between serum irisin and vascular calcification in HD patients PMID: 29490308
The pooled data indicated that the levels of irisin were at least 45.78 ng/ml higher in patients with polycystic ovary syndrome than that in the healthy controls. The current meta-analysis suggested that irisin might contribute to the development of PCOS independent of insulin resistance. PMID: 29069945
With adjustment for body mass index, circulating irisin in polycystic ovary syndrome patients seems comparable to healthy controls. [meta-analysis] PMID: 29217128
serum irisin levels were lower in Peritoneal Dialysis patients with Protein Energy Wasting than those of the patients without PEW PMID: 29248911
Data suggest that decreased irisin levels are associated with metabolic syndrome in prepubertal children; irisin may be a biomarker for metabolic syndrome in prepubertal children. This study was conducted in Seoul, Republic of Korea. PMID: 28904307
Findings revealed that irisin may play a critical role in the IL-6-induced epithelialmesenchymal transition of osteosarcoma cells via the STAT3/Snail signaling pathway. PMID: 29048621
FNDC5 gene interactions with candidate genes FOXOA3 and APOE PMID: 29143599
Irisin is negatively associated with serum testosterone in apopulation sample of men with Metabolic syndrome. PMID: 28759938
Irisin replenishment in mCaROCK1 mice partially reversed insulin resistance. PMID: 27411515
Suggest that irisin exerts beneficial effect on mood in COPD patients, possibly by inducing the expression of BDNF in brain areas associated with reward-related processes involved in by depression. PMID: 28744117
Modulation of Irisin and Physical Activity on Executive Functions in Obesity and Morbid obesity. PMID: 27476477
Obese children had significantly higher irisin and lower oxytocin levels than the healthy controls. PMID: 28077341
We determined FNDC5 polymorphisms frequencies in the Israeli population and demonstrated that maternal and neonatal FNDC5 rs726344 polymorphism is significantly associated with increased risk for preterm birth. Women bearing FNDC5 rs726344 GG genotype had 2.18 fold higher chance to deliver on time compared to AG and AA genotypes.Neonatal carrying FNDC5 rs726344 GG genotype had 2.24 fold higher chance to be born on time. PMID: 29408625
Lower levels of irisin are independently associated with elevated skin AF values, indicating that circulating irisin levels could be associated with AGEs accumulation, which is one of the reasons causing vascular complications in diabetic patients. PMID: 28408433
Association of Irisin Plasma Levels with Anthropometric Parameters in Children with Underweight, Normal Weight, Overweight, and Obesity. PMID: 28553647
irisin was significantly higher in obese than in control children, and was inversely correlated with Acrp30 and high molecular weight (HMW) oligomers; inverse correlation between Irisin and Acrp30 and, more significantly, between Irisin and HMW oligomers suggests that the two cytokines are closely connected PMID: 28385328
Irisin is an oxidative stress marker and a metabolic protective hormone. PMID: 28277125
Mild cold exposure increased vasoconstriction with a drop in in-the-ear temperature and these were related. Greater irisin was related to a greater fasting fat oxidation in the absence of shivering PMID: 27006247
Activation of the nuclear receptor constitutive androstane receptor (CAR) induced FNDC5 mRNA expression in the liver. PMID: 27007446
HCC-liver tissue over-expressed FNDC5/Irisin in association with gene expression of mediators involved in lipogenesis, inflammation and cancer, suggesting a possible protective role of the hormone from the liver damage. PMID: 28012856
The secretion of FNDC5 from myotubes and beta-cells in response to exogenous fatty acids, the effects of recombinant FNDC5 on insulin biosynthesis and glucose-stimulated insulin secretion, and beta-cell apoptosis are reported. PMID: 28724742
Irisin regulates the number and function of endothelial progenitor cells via the PI3K/Akt/eNOS pathway in mouse model of diabetes mellitus. PMID: 27002278
there is a correlation between sport performance, insulin sensitivity, and irisin levels PMID: 28386566
results firstly revealed that irisin mitigated oxygen-glucose deprivation-induced neuronal injury in part via inhibiting ROS-NLRP3 inflammatory signaling pathway, suggesting a likely mechanism for irisin-induced therapeutic effect in ischemic stroke. PMID: 28961497
These data indicate that increased irisin levels may have protective roles in liver cancer cells through partial activation of the PI3K/AKT pathway, which may facilitate liver cancer progression and decrease the sensitivity to chemotherapy. PMID: 28867187
Decreased serum irisin levels are related to emphysema in patients with COPD and involved in epithelial apoptosis, resulting in emphysema. PMID: 28424548
Irisin levels are lower in MI and CAD implying that their production may depend on myocardial blood supply. PMID: 28732565
findings suggest that irisin plays an important role in metabolic disorders and may be affected by physiopathological status. PMID: 28732566
The FNDC5 rs3480 variant is associated with protection from clinically significant fibrosis in patients with NAFLD, while irisin expression is correlated with the severity of NAFLD and may be involved in extracellular matrix deposition. These data suggest that irisin is involved in regulation of hepatic fibrogenesis. PMID: 28472477
The median irisin levels were determined higher in T1DM group compared to the control one. PMID: 28222023
The modulation of body composition and muscle strength induced by 16-week of resistance training in older women with and without obesity is not associated with changes in circulating Irisin levels. PMID: 28244561
serum irisin levels were correlated with anthropometric and metabolic markers of obesity and type 2 diabetes mellitus . PMID: 27220658
Plasma irisin modestly increases during moderate and high-intensity afternoon exercise in obese females. PMID: 28125733
correlation between irisin and adiposity related factors suggests that that in the case of this clinical model, irisin is regulated by adiposity and not by GH PMID: 27472279
Collectively, our study identified serum irisin as a predictive biomarker for 1-year all-cause mortality in acute heart failure patients though large multicenter studies are highly needed. PMID: 28595171
T2DM and diabetic nephropathy are associated with decreased levels of irisin. FNDC5 rs16835198 TT genotype associates with decreased risk of T2DM in Egyptians with no effect on renal complications. Also, G allele has insulin desensitizing action with no association with circulating irisin levels. PMID: 28479383
irisin might serve as a possible signal for linking body fat/muscle mass with the hypothalamic center governing reproductive function PMID: 27692156
Results indicate that cytokines might predict irisin concentration in mothers and their offspring. PMID: 27828992

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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