Recombinant Human Neurotrophin-4 Protein (NTF4), Active

Name: Recombinant Human Neurotrophin-4 Protein (NTF4), Active
Brand: Beta LifeScience
SKU: BLC-06017P
Availability: InStock

Recombinant Human Neurotrophin-4 Protein (NTF4), Active

Collections: Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Neurotrophin-4 Protein (NTF4), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of >2.0x10 4 IU/mg
Uniprotkb	P34130
Target Symbol	NTF4
Synonyms	GLC10; GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT 4/5; NT 5; NT-4; NT-5; NT4; NT4/5; NT4P; NT5; NTF4; NTF4_HUMAN; NTF5
Species	Homo sapiens (Human)
Expression System	E.Coli
Tag	Tag-Free
Complete Sequence	M+GVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A
Expression Range	81-210aa
Protein Length	Full Length of Mature Protein
Mol. Weight	14.1 kDa
Research Area	Neuroscience
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 5.5
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	derived survival factor for peripheral sensory sympathetic neurons.
Subcellular Location	Secreted.
Protein Families	NGF-beta family
Database References	HGNC: 8024 OMIM: 162662 KEGG: hsa:4909 STRING: 9606.ENSP00000301411 UniGene: Hs.266902
Associated Diseases	Glaucoma 1, open angle, O (GLC1O)
Tissue Specificity	Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Gene Functions References

Elevated intra-amniotic levels of docosahexaenoic acid and arachidonic acid in the mid-trimester are correlated with increased NT4 concentrations. PMID: 24607879
The NTF4 variants p.Gly157Ala and p.Ala182Val have been shown to be functional mutations, occurring in 2 of a total of 720 Chinese primary open-angle glaucoma patients. PMID: 22815630
NT-4/5 expression is associated with atrophy of the brain-parenchymal fraction measured by magnetic resonance imaging methods in patients with relapsing-remitting multiple sclerosis. PMID: 22036954
Suggest that a dysregulated TrkB/NT4/5 axis may contribute to several of the pathological lesions associated with pulmonary fibrosis, including type 2 alveolar cell hyperplasia and the proliferation of fibroblasts. PMID: 21330466
neurotrophin 4 contributes to breast cancer cell survival and can serve as prospective target to inhibit tumor growth PMID: 21350004
In vitro follicular assembly is significantly increased in fetal ovaries cultured with NT-4. PMID: 20447632
Identification of a single mutation in our study suggests that NTF4 mutations are a rare cause of primary open-angle glaucoma (0.6%, 95%CI 0.02%-3.16%) in Chinese people. PMID: 20806036
present data indicate a lack of involvement of variations in NTF4, VAV2, and VAV3 with glaucoma pathogenesis in an Indian population. PMID: 20463313
Expression of human NTF4 was unchanged during gestation in the developping ovary. PMID: 20175187
No evidence of association of heterozygous NTF4 mutations in patients with primary open-angle glaucoma. PMID: 20215012
A mechanism to understand the defect associated with variant BDNF and provide a framework two ligands for BDNF and NT-4. PMID: 15987945
Re-expression of the p75NTR appears to partially reverse de-differentiation of prostate cancer cells by up-regulating the expression of CRABPI for localized sequestration of retinoids. PMID: 16316409
Results revealed the expression of NT-4/5 mainly in oocytes and, in a minority of samples, also in the granulosa cells (GCs). PMID: 16648150
These novel data demonstrate that neurotrophins influence ASM [Ca(2+)](i) and force regulation and suggest a potential role for neurotrophins in airway diseases PMID: 16648236
Neurotrophin 4/5 plays a role in the proliferation and differentiation of periodontal ligament cells. PMID: 18980528
Data show that the levels of NT-4/5 levels are significantly higher in bipolar disorder patients than in controlsNT-4/5. immunocontent alterations in bipolar disorder. PMID: 19081579
NTF4 mutations impair neurotrophin-4 signaling in patients with primary open-angle glaucoma and lead to decreased activation of TrkB PMID: 19765683

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.