Recombinant Human pro-BDNF Protein

Name: Recombinant Human pro-BDNF Protein
Brand: Beta LifeScience
SKU: BL-1757NP
Availability: InStock

BL-1757NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Cytokines, Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Pro-Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala19-Arg247(R125A,R127A,R128A) is expressed.
Accession	P23560
Synonym	Brain-Derived Neurotrophic Factor; BDNF; Abrineurin
Gene Background	The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
Molecular Mass	25.6 KDa
Apmol Mass	28 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
Subcellular Location	Secreted.; [BDNF precursor form]: Secreted.
Protein Families	NGF-beta family
Database References	HGNC: 1033 OMIM: 113505 KEGG: hsa:627 STRING: 9606.ENSP00000414303 UniGene: Hs.502182
Associated Diseases	Bulimia nervosa 2 (BULN2); Congenital central hypoventilation syndrome (CCHS)
Tissue Specificity	Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Gene Functions References

The sample consisted of 181 suicide cases (47 early suicide cases, 134 late suicide cases) and 162 controls. Patients with the BDNF Met allele had a 1.42-fold increased risk of suicide when compared with the Val allele carrier. When a suicide case is stratified by occurrence time, patients with the Met/Val or Met/Met genotype had a 2.48-fold increased risk of committing suicide early than those with the Val/Val genotype. PMID: 29734216
Low brain derived neurotrophic factor may contribute to the pathogenesis of schizophrenia, but maybe not to its cognitive impairments. PMID: 29482040
In a 12-year longitudinal population-based sample of older adults (n = 2,218), we used growth curve modeling to investigate whether the benefits of physical activity on cognitive preservation differed by BDNF genotype and sex across multiple cognitive domains including processing speed, attention, working memory, and episodic verbal memory. PMID: 29402782
Mn-SOD V allele carries a worse outcome profile after stroke, relating to nitrosative stress, inflammatory, and apoptotic response and associated with a BDNF reduction. PMID: 30150066
while the BDNF Val66Met Megroup displayed significant SICI reduction in the bilateral M1 in response to motor training, short-interval intracortical inhibition (SICI) remained unchanged in the BDNF Val66Met group. PMID: 29856758
Our study provides evidence for the correlation between the BDNF variant rs6265 and emotional symptoms in the early phase after mild traumatic brain injury. PMID: 29357818
These results indicate that genetic variant Met66 decreased the serum BDNF levels in combination with self-reported risk-taking propensity among heroin users. PMID: 30134233
serum BDNF levels and the BDNF Val66Met polymorphism in healthy young adults were associated with the sleep pattern on weekends but not with that on weekdays, suggesting that the systems involved in BDNF control may be linked to endogenous sleep characteristics rather than the socially constrained sleep schedule in healthy young adults. PMID: 29944703
Neurotrophic factors and hippocampal activity in PTSD PMID: 29799860
upregulation of PAI-1 may be a critical mechanism underlying insufficient neurotrophic support and increased neurodegeneration associated with AD. Thus, targeting BDNF maturation through pharmacological inhibition of PAI-1 might become a potential treatment for AD. PMID: 28132883
This study shows the genetic correlates of early trauma in a group of schizophrenia patients (BDNF Met carriers). PMID: 28711474
This study demonstrated significant differences in the blood levels of BDNF between people with epilepsy and healthy subjects. PMID: 30140987
Study shows that brain-derived neurotrophic factor concentrations in serum and peritoneal fluid were significantly higher in women with endometriosis with pain compared to women with endometriosis without pain. PMID: 28954602
Met allele of the Val66Met polymorphism in brain derived neurotrophic factor is associated with lower BMI-SDS in children PMID: 28960774
platelet BDNF and SERT do not specifically underlie psychosocial deficits in stage Huntington's Disease, while higher BDNF storage in delayed mild symptoms PMID: 30039833
Direct within family analysis showed that fathers with the Met allele were more likely than Val/Val carriers to exhibit differential parenting toward twins who differed in their prosocial behavior. The same pattern of findings was found with mother-rated and experimentally assessed prosociality. PMID: 28523227
The results of this study suggest that the Val66Met polymorphism does not predict long-term, functional mobility following stroke. PMID: 29480080
polymorphisms in BDNF gene including rs925946, rs10501087, rs6265 and rs988712 can be considered as genetic determinants of obesity (systematic review and meta-analysis). PMID: 28818748
Single nucleotide polymorphism found in the BDNF-AS (BDNF antisense RNA [nonprotein coding]) gene may be related to the decreased plasma brain derived neurotrophic factor levels found in frail elderly people. PMID: 27449141
The pattern of low BDNF and high inflammation in MDD may be influenced by the Val66Met polymorphism; the association of a polymorphism in the BDNF gene with inflammatory markers in addition to BDNF levels suggests an interaction between these systems. PMID: 28656803
Angiogenin contributes to angiogenesis induced by Brain-derived neurotrophic factor (BDNF). PMID: 29573867
Findings demonstrated that polymorphism Val66Met is not associated with temporal lobe epilepsy caused by hippocampal sclerosis, epilepsy-related factors and psychiatric comorbidities in this selected group of patients. PMID: 30015148
Study suggested that BDNF 196 G>A polymorphism may be a genetic marker for predicting insulin resistance before initiating risperidone treatment in autism spectrum disorder patients. PMID: 29369497
No significant difference was found in the BDNF Val66Met polymorphism between patients with GAD and healthy controls, nor was this polymorphism significantly associated with antidepressant drug efficacy for GAD PMID: 29446659
Its single-nucleotide polymorphisms involves in dopaminergic metabolism and motor and cognitive function in older adults. PMID: 29525179
BDNF rs11030101 and BDNF rs61888800are associated with change of temperament scores in a clinical sample of subjects with major depression (MDD), who received selective serotonin reuptake inhibitor treatment. PMID: 29310728
We found that harsh parenting predicted an increased error-related negativity only among children with a methionine allele of the BDNF genotype, and evidence of moderated mediation: the ERN mediated the relationship between parenting and internalizing diagnoses and dimensional symptoms only if children had a methionine allele. PMID: 28427482
Findings presented here suggest a strong influence of seasonality on depression outcome and BDNF expression in atopic dermatitis and psoriasis PMID: 27409526
Serum BDNF was significantly lower in patients with Parkinson's disease than in patients with essential tremor and controls. PMID: 29350074
The purpose of this systematic review was to provide a comprehensive analysis of the available clinical trials analyzing, in seniors, the effect of interval aerobic training (IAT) and continuous aerobic training (CAT) on peripheral brain-derived neurotrophic factor (BDNF) concentration. PMID: 28498065
findings suggested that BDNF modulates graunlosa cell functions and the action probably mediated by FSHR-coupled signaling pathway, to affect aromatase-mediated steroidogenesis. PMID: 28282971
The functional Val66Met BDNF polymorphism is not associated with BDNF serum levels in acute episode of schizophrenia and depression. PMID: 29331787
This study demonstrated that a significantly higher incidence of defective BDNF expression in granule layers of the cerebellar cortex. PMID: 29174061
combining cognitive and physical exercise had no additional or synergistic effect on peripheral BDNF levels compared with physical exercise alone PMID: 29842831
Review/Meta-analysis: suggests a lower risk of ischemic stroke for the GG genotype of BDNF rs6265. PMID: 29449128
that the BDNF SNP rs1157659 interacted with mild traumatic brain injury to predict hippocampal volume PMID: 28755387
found that though there was no significantly difference in peripheral BDNF levels between ADHD patients and control groups overall, BDNF levels were significantly higher in males with ADHD compared with controls. PMID: 29132072
found evidence that supported the hypothesis that BDNF Val66Met polymorphism moderated the relationship between stress and depression. PMID: 29102837
the BDNF Val66Met polymorphism is associated with functional tuning of behaviorally-relevant frontolimbic circuitry, particularly involving the ventromedial prefrontal cortex, during higher-order learning. PMID: 28867340
MicroRNA-103 suppresses glioma cell proliferation and invasion by targeting BDNF. PMID: 29257320
High expression levels of BDNF was observed in cervical cancer.BDNF role in epithelial-mesenchymal transition, migration and invasion of cervical cancer. PMID: 29345295
There was a statistically significant change in BDNF levels post-chemotherapy in early-stage breast cancer patients, and plasma BDNF levels were associated with self-perceived concentration deficit in patients receiving chemotherapy. PMID: 29258453
Meta-analysis/Review: patients with ischemic stroke at high risk of post stroke depression have lower BDNF levels at the early stage of stroke. PMID: 29128330
Coronary artery disease patients exhibited significantly lower plasma BDNF and higher vWF levels than those of control patients. PMID: 29409455
Brain-derived neurotrophic factor (BDNF) was found to be the downstream target of miR-107 in breast cancer. PMID: 27813254
This study showed that in first-episode psychosis subjects, with global DNA hypo-methylation and reduced BDNF gene-expression. PMID: 29056292
down-regulated in cord blood by prenatal smoking PMID: 28130959
BDNF/TrkB axis plays a role in epithelial mesenchymal transition promoting the acquisition of (myo)fibroblast cell phenotype in idiopathic pulmonary fibrosis. PMID: 28938915
Results show that Lnc RNA BDNF-AS inversely regulates the expression level of BDNF which modulates high-glucose induced apoptosis in human retinal pigment epithelial cells. PMID: 28657668
This study show a linear relationship between the BDNF Val66Met genotypes and plasma BDNF levels in Caucasian depressed patients. PMID: 28848102

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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