Recombinant Human PTK7 Protein

Name: Recombinant Human PTK7 Protein
Brand: Beta LifeScience
SKU: BL-1198SG
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Enzymes, Kinase, Recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	NM_002821
Synonym	CCK-4; CCK4
Background	PTK7 or pseudo protein tyrosine kinase 7 is a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. PTK7 lacks detectable catalytic tyrosine kinase activity but is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion (1). PTK7 interacts with beta-catenin and functions upstream from glycogen synthase kinase 3. PTK7 has been isolated from colon carcinoma and was designated colon carcinoma kinase-4 (2). In conjunction with other T cell markers, PTK7 has utility as a biomarker for detecting minimal residual disease of T-ALL in the bone marrow.
Description	Recombinant human PTK7 (726-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	726a.a.-end
Molecular Weight	~66 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human PTK7 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Subcellular Location	Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
Protein Families	Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database References	HGNC: 9618 OMIM: 601890 KEGG: hsa:5754 STRING: 9606.ENSP00000230419 UniGene: Hs.90572
Tissue Specificity	Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.

Gene Functions References

miR2055p is involved in the proliferation, migration and invasion of colorectal cancer cells through inhibiting PTK7. PMID: 29488611
Higher expression of PTK7 significantly indicates worse prognosis in human malignancies PMID: 28924970
Inhibition of PTK7 by siRNA treatment significantly decreases the viability of atypical teratoid rhabdoid tumors (ATRT)patient-derived tumor cell lines.These studies provide the groundwork for future preclinical in vivo studies aiming to investigate the efficacy of PTK7 inhibition on ATRT tumor growth PMID: 28442586
PTK7 localization and protein stability is affected by canonical Wnt ligands. PMID: 28420671
These findings demonstrate that PTK7 upregulates MMP9 through activation of AP-1 and NF-kappaB and, thus increases invasive properties of ESCC cells. PMID: 27689325
PTK7 overexpression is associated with esophageal squamous cell carcinoma. PMID: 28545451
Our data indicate that PTK7 protein expression is associated with the prognosis of oral tongue squamous cell carcinoma PMID: 28547980
PTK7 expression was correlated with tumor differentiation (P=0.027), lymph node metastasis (P=0.005), distant metastasis (P=0.001) and TNM stage (P=0.028) of colorectal cancer patients PMID: 27499181
High PTK7 expression is associated with radioresistance in esophageal squamous cell carcinoma. PMID: 27557627
PTK7 enriches in self-renewing, multipotent stem cells of the human colon. PMID: 26549850
findings implicate PTK7 as a risk factor for NTDs and provide additional evidence for a pathogenic role of PCP signaling in these malformations PMID: 26368655
overexpression in PTK7-negative cancer cells led to increased metastatic events. PTK7 expression thus represents a potential prognostic biomarker and a novel therapeutic target in CRC. PMID: 25962058
data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID: 25986862
PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID: 25204555
The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID: 24741114
Expression level of PTK7 was significantly decreased from benign to malignant ovarian epithelial tumors. survival analysis showed that patients with negative expression of PTK7 protein had poorer outcome than those with positive expression. PMID: 25550828
PTK7 acts as a cancer-related gene and may be a potent prognostic marker for Hepatocellular carcinoma(HCC). PMID: 25796105
results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer PMID: 25006253
PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma (ICC) cells and leads to a poor prognosis in ICC patients. PMID: 24587299
PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PMID: 24409301
This work defines PTK7 as a highly and specifically expressed gene in adenocarcinoma and a potential therapeutic target in this subset of NSCLC. PMID: 24654231
Increased expression of PTK7 was inversely correlated with overall survival. PMID: 23663482
in patients treated by chemotherapy, patients with PTK7-negative tumors seemed to have better DFS than those with PTK7-positive tumors, particularly for patients treated with only anthracycline-therapy drugs PMID: 24023307
PTK7 is a novel marker of epithelial to mesenchymal transitions PMID: 23209741
cleavage of the PTK7 ectodomain by an ADAM proteinase was coupled with the membrane type-1 matrix metalloproteinase (MT1-MMP) cleavage of the PKP(621) downward arrowLI site in the seventh Ig-like domain of PTK7. PMID: 23095747
PTK7 has utility as a biomarker for detecting minimal residual disease of T cell acute lymphoblastic leukemia in the bone marrow PMID: 22898210
a novel role for PTK7 in the tumorigenesis via generation of PTK7-CTF2 by sequential cleavage of ADAM17 and gamma-secretase. PMID: 22665490
aberrations in the membrane type 1 matrix metalloproteinase/PTK7 axis are detrimental to cell movements that shape the body plan PMID: 21518755
Observations may indicate a role for PTK7 in cell proliferation and cell apoptosis and may provide a potential therapeutic pathway for the treatment of a variety of cancers. PMID: 21103379
Protein Tyrosine Kinase 7 is required for beta-catenin-dependent transcriptional events induced by canonical Wnt ligands PMID: 21132015
Results indicate that PTK7 is a major target of MT1-MMP. PMID: 20837484
PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and represents an independent prognosis factor in patients treated with induction chemotherapy. PMID: 20558616
cloning, sequencing and expression of gene and its splice variants PMID: 12427550
Lack of a thermodynamically meaningful self-association propensity for the CCK4 transmembrane domains demonstrates that the conserved GxxxG motif is not sufficient to drive CCK4 transmembrane helix-helix interactions. PMID: 15683231
mechanism of PTK7 upregulation in colorectal cancer PMID: 17671748
PTK7 plays an important role not only in tube formation, migration, and invasion of endothelial cells but also in angiogenesis. PMID: 18471990

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.