Recombinant Human Resistin (RETN) Protein

Name: Recombinant Human Resistin (RETN) Protein
Brand: Beta LifeScience
SKU: BL-1739SG
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	Q9HD89
Synonym	FIZZ3, Adipose tissue-specific secretory factor, ADSF, HXCP1, RSTIN, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3
Background	Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down-regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target-tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90% in 3T3-L1 adipocytes.
Description	Recombinant Human Resistin (RETN) was produced in E. coli. This protein is purified with our unique purification methods.
Source	E.coli
Molecular Weight	10.0 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human Resistin (RETN) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Promotes chemotaxis in myeloid cells.
Subcellular Location	Secreted.
Protein Families	Resistin/FIZZ family
Database References	HGNC: 20389 OMIM: 605565 KEGG: hsa:56729 STRING: 9606.ENSP00000221515 UniGene: Hs.283091
Tissue Specificity	Expressed in white adipose tissue (at protein level). Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood. Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues

Gene Functions References

The results of this study indicated that resistin gene polymorphisms might affect the genetic predisposition of rheumatoid arthritis in Chinese population. PMID: 29978502
It was demonstrated that high resistin expression was predominantly observed in lung adenocarcinoma tissues. It is associated with a more malignant clinicopathological status and poorer survival. Resistin could promote A549 and H1975 tumor cell proliferation, migration and invasion while inhibit their apoptosis in vitro. PMID: 30272365
A multi-cytokine resistin pathway is operational in humans, and it plays a substantial role in cardiovascular events in high-risk individuals. PMID: 28290549
An increase in resistin concentration expressed also as resistin/BMI, and resistin/adiponectin ratios, observed in children with cystic fibrosis may suggests that this adipokine is involved in the inflammatory process underlying the disease and is related to worse spirometric parameters describing airways obstruction. PMID: 29465158
n T2 mice, the mRNA expression of Retn showed a moderate up-regulation (fold change=8.32; p=0.0019) in the adipose tissues. Iapp expression was also significantly up-regulated (fold change=9.78; p=0.012). Moreover, a 6.36-fold up-regulation for Drd5 was observed in the adipose tissues of T2 mice PMID: 29936463
RETN and CAP1 polymorphisms and gene expression may be potential biomarkers for breast cancer risk PMID: 29641286
resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
Study provides clear evidence that resistin is a clinically relevant endogenous ligand for TLR4, which promotes tumor progression via TLR4/NF-kappaB/STAT3 signaling. PMID: 28991224
women with gestational diabetes showed significantly low adiponectin and high resistin levels when compared with control group. PMID: 29207892
increased levels of resistin in erosive and non-erosive hand osteoarthritis PMID: 29105498
Resistin promotes a shift from proliferation to apoptosis in vascular smooth muscle cells and macrophage co-culture systems with cellular composition similar to that found in vulnerable regions of plaques. PMID: 29361366
The inverse association between serum resistin and n-3 PUFA intake was strongest in SNP-420 G/G genotype in the Japanese cohort. PMID: 29044636
Our results do not support a relationship among resistin levels, obesity and insulin resistance in humans PMID: 29280648
rs1862513 and rs3745368 in resistin genes are not associated with the presence of MetS in a Han Chinese population. PMID: 29386698
For T2D, when the subjects were separated by resistin into tertiles, elevated resistin was associated with a benefit for the Castelli 1 index PMID: 28760596
The -420C>G resistin gene polymorphism appears to be associated with more severe stroke and higher in-hospital mortality in patients with acute ischemic stroke. Higher leptin levels appear to be related to favorable functional outcome. PMID: 29217361
Resistin contributes to the pathogenesis of rheumatoid arthritis by increasing chemokine production by fibroblast-like synoviocytes PMID: 29191223
Studies identify a critical role for hRetn in blocking lipopolysaccharide function with important clinical significance in helminth-induced immunomodulation and sepsis. PMID: 29133417
Plasma resistin did not show any association with the degree of vascular damage in non-hypersomnolent obstructive sleep apnoea patients. PMID: 27256793
In anorexia nervosa subjects, GG genotype of RETN c.-180 was significantly more frequent than in control group. PMID: 28759190
serum and follicular fluid resistin levels were significantly higher in the endometriosis group PMID: 29442466
Our data suggest that adiponectin and resistin are altered in non-obese chronic migraineurs. PMID: 27553954
Studied the association of resistin (RETN); single nucleotide polymorphisms (SNPs) and serum RETN levels in obese and non-obese Tunisians. PMID: 28393393
This study has shown Resistin, and Visfatin synergistically increased gastric cancer cell proliferation and enhanced the telomerase gene expression, showing that these two hormones in gastric cancer tissue could cooperatively accelerate cancer cell growth PMID: 29228527
resistin is differentially expressed in endometrial tissues from women with endometriosis and imply a role for resistin in endometriosis-associated pelvic inflammation PMID: 28681517
Resistin, but not copeptin levels are higher in acute ischemic stroke patients early after the stroke onset, than in age and gender matched stroke-free controls. Moreover, higher copeptin concentrations are predictive of poor short term functional outcome after ischemic stroke PMID: 28845746
Significant up-regulation of PBMCs CAP1, CD36 mRNA and plasma resistin found in significant coronary artery disease, as well as in nonsignificant coronary artery disease compared to control group, indicates that resistin could be able to exert its effects stronger on cells with up-regulated CAP1 mRNA thus contributing atherosclerosis development. PMID: 28707728
Results demonstrate an association between RETN gene variants and risk of rheumatoid arthritis disease. RETN SNPs were significantly associated with clinical therapies and C-reactive protein serum marker of rheumatoid arthritis in the Chinese Han population. PMID: 29561430
Studied adiponectin (ADIPOQ), leptin (LEP), leptin receptor (LEPR), and resistin (RETN) single nucleotide polymorphisms and their association in obesity. Results showed that ADIPOQ 4522C PMID: 28195351
Results show that adiponectin, resistin and visfatin are present in osteophytes but have no direct influence on osteophyte development. Although these adipokines induce several inflammatory mediators, they do not affect osteoblast- or chondrocyte-related mechanisms of osteophyte development. PMID: 27884778
Fine-tuned by RETN SNPs, intrahepatic, multi-cellular resistin reinforced IFNL3 in eliminating HCV via immunomodulation to counteract pro-inflammation. PMID: 27477870
Dementia of vascular origin is characterized by elevated resistin levels. PMID: 28421328
Resistin probably plays a role in the pathogenesis of hepatic insulin resistance and aggravates pathologic changes in the liver of patients with non-alcoholic fatty liver disease PMID: 29363914
Resistin and NGAL correlate with expression of endothelial cell adhesion molecules in sepsis. PMID: 28424824
the role of resistin (an adipokine) in the development of gestational diabetes mellitus PMID: 28685604
high resistin levels are associated with increased obesity-related cancer risk [meta-analysis] PMID: 27509174
Results showed that the RETN SNP rs3219175 with AG or at least 1 A allele was associated with a higher risk of lung cancer than wild-type (GG) carriers. Moreover, the RETN SNP rs3219175 with AG or AG + AA alleles was associated with a higher risk of distant metastasis than that in patients carrying GG alleles. PMID: 29384942
Report decreased serum resistin level in Grave's disease patients. Suggest that serum resistin is primarily secreted from circulating neutrophils and down-regulated by excessive thyroid hormones in GD patients. PMID: 27637079
Change in salivary resistin level was not significantly associated with periodontal outcomes. In obese Malaysians, NSPT significantly improved PS and GBI, and improved PPD and CAL for shallow and moderately deep pockets but not for deep pockets PMID: 28049964
Therefore, rs1423096 and rs10401670, in addition to SNP-420 and SNP-358, were identified as possible functional variants affecting circulating resistin by the genome-wide search in the Japanese population. PMID: 27664181
In this meta-analysis, serum resistin levels are associated with and increased risk of acute cerebral infarction in Asians, but not in Caucasians. PMID: 26899574
the mutant genotype of two SNPs of the RETN gene (rs1862513 and rs10401670) was associated with a lack of change in resistin secondary to biliopancreatic diversion PMID: 27118275
Serum resistin level was higher in CKD ( Chronic Kidney Diseases ) patients compared to control subjects. PMID: 25385911
The present study suggests that the G/G genotype of RETN rs1862513 could be a predictor of the reduction of HOMA-IR, insulin, fasting glucose and LDL cholesterol secondary to a hypocaloric diet in obese subjects. PMID: 28064279
The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
Results provide evidence that RETN SNPs were functional and associated with the levels of serum creatinine and cholesterol. PMID: 29101068
Resistin-associated VSMC dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID: 27573736
Adiponectin and resistin show an additive independent effect on all-cause mortality in patients with type 2 diabetes PMID: 27175608
Amendment of the cytokine profile in macrophages subsequent to their interaction with smooth muscle cells: Differential modulation by fractalkine and resistin. PMID: 27180200
The strongest OR was for CXCL8 (interleukin-8) in serum (96.8, 95% CI: 11.9-790.2). Of these 15 markers, 6 were also significantly elevated in serum from Chile (CCL20, C-reactive protein, CXCL8, CXCL10, resistin, serum amyloid A). PMID: 27173614

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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