Recombinant Human Serum Amyloid A Protein

Name: Recombinant Human Serum Amyloid A Protein
Brand: Beta LifeScience
SKU: BLA-1087P
Availability: InStock

Collections: Cytokines and growth factors, Other cytokines, Recombinant proteins

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Product Overview

Host Species	Human
Accession	P0DJI8
Synonym	Amyloid fibrils SAA 2 Serum amyloid A1 isoform 2 TP53I4 Amyloid fibril protein AA Amyloid protein A Fibrils MGC111216 OC OTTMUSP0000004557 PIG 4 PIG4 SAA SAA 1 SAA_HUMAN SAA1 SAA2 serum amyloid A Serum amyloid A 1 Serum amyloid A protein precursor Serum amyloid A1 isoform 1 Serum amyloid protein A(4-101) Tumor protein p53 inducible protein 4
Description	Recombinant Human Serum Amyloid A Protein was expressed in E.coli. It is a Full length protein
Source	E.coli
AA Sequence	MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY
Molecular Weight	12 kDa
Purity	>= 98% SDS-PAGE.>=98% by SDS-PAGE and HPLC analyses.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
Formulation	Lyophilised
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycle.

Target Details

Target Function	Major acute phase protein.
Subcellular Location	Secreted.
Protein Families	SAA family
Database References	HGNC: 10513 OMIM: 104750 KEGG: hsa:6288 STRING: 9606.ENSP00000348918 UniGene: Hs.632144
Associated Diseases	Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
Tissue Specificity	Expressed by the liver; secreted in plasma (at protein level).

Gene Functions References

SAA1 can be produced in human fetal membranes, which can be greatly induced in the presence of proinflammatory cytokines and glucocorticoids thereby producing effects associated with parturition PMID: 28386088
High SAA1 expression in the stromal component is associated with pancreatic tumors. PMID: 29351990
Data suggest that anti-inflammatory capacity of HDL (high-density lipoprotein) from patients with type 2 diabetes and diabetic nephropathy is impaired; this effect might be attributable to SAA enrichment in HDL in these patients; up-regulation of SAA in HDL appears to be associated with increased risk of diabetic angiopathy or vasculitis in such patients. PMID: 28760652
The frequencies of the CC genotype and the C allele of SAA rs12218 were higher in participants with ischemic stroke than in the control group. The frequencies of the AG genotype and the G allele of rs2468844 were higher in participants with ischemic stroke than in the control group. Multiple logistic regression analysis revealed the significance of the rs12218 in males and in large-artery atherosclerosis group. PMID: 28870546
we show that astrocytoma patients have increased levels of serum SAA and SAA1 is expressed and secreted in glioblastoma, and its co-expression with tumor-related genes supports its involvement in glioblastoma angiogenesis and progression. PMID: 28283801
These results suggested that the C-terminal truncation of human SAA accelerates amyloid fibril formation. PMID: 29288051
SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin. PMID: 26900010
Data suggest that serum amyloid A (SAA) immunoreactivity in tumor-associated macrophage (TAM) is associated with worse recurrence-free survival, and is perhaps a therapeutic target for breast cancer. PMID: 27058895
Findings indicate that in Lyme disease patients, circulating CRP and SAA (Serum Amyloid A) levels are highest when the concentration of spirochetes is greatest in skin and/or blood and that levels decline after the dissemination of the organism to extracutaneous sites in subsequent stages of infection. PMID: 27585799
The Serum amyloid A (SAA)/formyl peptide receptor-like 1 (FPRL1) contributed to pathogenesis of psoriasis by promoting keratinocyte proliferation and inflammation, thus providing a potential therapeutic target for disease therapy. PMID: 27910163
High SAA expression is associated with lung cancer. PMID: 27809798
SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB PMID: 28278182
Novel truncated form of serum amyloid A is elevated in the plasma of Kawasaki disease (KD) when compared with Febrile control subjects. Future studies will evaluate its relevance as a diagnostic biomarker and its potential role in the pathophysiology of KD. PMID: 27271757
SR-A1 suppresses lung cancer metastasis by downregulating SAA1 production in tumor-associated macrophages (TAM). PMID: 28202524
The SAA1.1 allele was found in four familial Mediterranean fever patients, including two with AA amyloidosis. PMID: 27150194
genetic variants at the CRP and SAA1 loci independently affect both CRP and SAA levels, and their respective circulating levels act as suppressors. PMID: 27313400
Serum amyloid A could represent a novel marker of primary unexplained recurrent early pregnancy loss because affected women exhibited elevated levels of this protein. PMID: 28099717
The polymorphism of SAA1 is not associated with susceptibility and severity of Familial Mediterranean Fever in Egyptian children PMID: 27300189
The findings identify SR-BII as a functional SAA receptor that mediates SAA uptake and contributes to its proinflammatory signaling via the MAPK-mediated signaling pathways. PMID: 28423002
Correlate MEFV genotype and the SAA1 polymorphisms with the clinical manifestations of familial Mediterranean fever and the occurrence of amyloidosis in a large cohort of Armenian patients. PMID: 27791951
Study found increased SAA concentration in patients with sarcoidosis. PMID: 26919159
the detection of residues 76 and 77 of SAA (AA76) may alter the ability to diagnose AA amyloidosis. PMID: 27098620
The results demonstrated that SAA upregulated Visfatin expression in cultured RAW264.7 macrophages and in the primary monocytes. PMID: 27006946
Structure, function and SAA1 gene polymorphisms PMID: 26945629
Serum Amyloid A induces inflammation, proliferation and cell death in activated hepatic stellate cells. PMID: 26937641
Amyloid A overexpressed in renal cell carcinoma patients and can serve as a prognostic marker. PMID: 26750935
incorporation of SAA into HDL preparations reduced antiinflammatory properties but not to the same extent as HDL from AgNO3-injected mice PMID: 26642365
human SAA is possibly only expressed in a subset of septic patients; SAA induces HMGB1 release via TLR4 and RAGE receptors; SAA supplementation worsens the outcome of lethal endotoxemia PMID: 26052716
The SAA1 rs12218 polymorphism was significantly more prevalent in ankylosing spondylitis patients with amyloidosis. PMID: 26300108
Autocrine/paracrine and recombinant human SAA1 via TLR4 stimulate a proinflammatory phenotype that is both part of the early phase of osteogenic differentiation and the development of senescence. PMID: 26135899
Serum amyloid A1alpha induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils. PMID: 26297794
These functional properties set SAA apart from well-characterized inflammatory factors, such as LPS and TNF-alpha, suggesting that it may play a homeostatic role during the course of inflammation. PMID: 26130702
The detailed analysis of the docking results points to the fact that the best serum amyloid A-Cystatin C (SAA-hCC) binding is achieved by the peptides inclined toward interacting via Lys90 and Arg 96 (with ffhCC Ser98 and Tyr102, respectively). PMID: 25736604
Our results showed PCT as a valuable marker of bacterial infections in febrile patients. PCT was superior to CRP, IL-6 or SAA in the early identification of bacterial infection PMID: 25963492
Mutations in genetic biomarker SAA1 gene related to amyloidosis processes may play a crucial role in chronic renal failure patients who require long-term hemodialysis. PMID: 25394530
The relative abundance of the N-terminal arginine truncation of SAA1.1 is significantly decreased in diabetes and negatively correlates with measures of glycemic and lipid control. PMID: 25607823
potential marker for detecting the activity of srcoidosis PMID: 25623898
Genetic polymorphisms in SAA1 are associated with coronary artery disease in the Han and Uygur populations in Western China. PMID: 25656165
High SAA serum level is associated with hepatocellular carcinoma. PMID: 25605163
HDL3 subpopulations in ST segment elevation myocardial infarction were enriched 10 times in SAA patients. PMID: 26037829
SAA has a role in activity of rheumatoid arthritis and risk of cardiovascular and renal involvement PMID: 25525305
SR-B1 and p38 MAPK are involved in signaling pathway of serum amyloid A-induced angiogenesis in rheumatoid arthritis. PMID: 25932604
Although SAA concentrations were elevated in the sera, there was no significant difference in these concentrations between familial Mediterranean fever patients and rheumatoid arthritis patients. PMID: 25240611
Zn deficiency enhances the acute phase response and particularly the JAK-STAT3 pathway, resulting in increased serum amyloid A production. PMID: 24732911
Serum amyloid A is a retinol binding protein that transports retinol during bacterial infection. PMID: 25073702
Data suggest that serum amyloid A (SAA) is a candidate for diabetic kidney disease therapeutic and biomarker discovery. PMID: 25531567
The CSF biomarker amyloid 1-42 is important and useful as part of the diagnostic procedure for detecting AD and other dementia in elderly patients displaying psychotic symptoms. PMID: 23597931
homozygous SAA1.5/1.5 genotype appears to be a recessive susceptibility gene, which has lost the antiangiogenic function, whereas SAA1.1 and SAA1.3 are the dominant alleles of the tumor suppressor phenotype. PMID: 24608426
keratinocyte-derived SAA triggers a key inflammatory mediator, IL-1beta, via NLRP3 inflammasome activation, providing new potential targets for the treatment of this chronic skin disease. PMID: 25231464
SAA1 gene expression in COPD patients is responsible for the secretion of this molecule in different cell types. PMID: 24884805

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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