Recombinant Human TFF3 Protein

Name: Recombinant Human TFF3 Protein
Brand: Beta LifeScience
SKU: BL-1714SG
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	Q07654
Synonym	Intestinal trefoil factor, ITF, Polypeptide P1.B, hP1.BRecombinant Human Trefoil Factor 3 (TFF3)
Background	Found in the gastrointestinal tract, the three Trefoil proteins, TFF1, 2, and 3 have been shown to play an important role in intestinal mucosal defence and repair. In addition to being expressed by goblet cells and in the uterus, TFF3 has also been shown to express in certain cancers providing a potential use as a molecular marker for those cancers.
Description	Recombinant Human TFF3 was produced in E. coli. This protein is purified with our unique purification methods.
Source	E.coli
Molecular Weight	6.8 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human TFF3 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Subcellular Location	Secreted, extracellular space, extracellular matrix. Cytoplasm.
Database References	HGNC: 11757 OMIM: 600633 KEGG: hsa:7033 STRING: 9606.ENSP00000430690 UniGene: Hs.82961
Tissue Specificity	Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular

Gene Functions References

TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgA nephropathy patients. PMID: 29987860
Authors hypothesised that TFF3 may involve regulatory networks of ncRNAs to exert its effects as a scattering factor. PMID: 28277538
The highly specific expression of TFF3 in mucinous ovarian cancer may help to further clarify potential relationships of tumors with mucinous histology PMID: 28470574
This is, to our knowledge, the first report of decreased expression of TFF3, SPDEF, KLF4, and goblet cell population in the colon of patients with HSCR. Altered goblet cell function may result in intestinal barrier dysfunction contributing to the development of HAEC. PMID: 29383490
High TFF3 expression is associated with oncogenicity and chemoresistance in hepatocellular carcinoma. PMID: 28445151
Results indicate diagnostic biomarker potential of Trefoil factor 3 (TFF3) promoter hypomethylation for prostate cancer (PC) as well as prognostic biomarker potential of TFF3 RNA expression. PMID: 28930171
ITF role in promoting intestinal epithelial cells migration through crosstalk between the ERK and JAK/STAT3 pathways PMID: 27616044
TFF3 levels were significantly elevated in patients with stage I (p= 0.001) and T1 gastric cancer (p= 0.013). PMID: 28453457
The present study demonstrated a negative association between the expression of miR75p and TFF3 in IBD lesional tissues and normal tissues. In conclusion, TFF3 was identified as a novel target of miR75p PMID: 28627600
trefoil factors family 3 may have a role in colorectal cancer PMID: 28405143
This study provides the first evidence that TFF3 is locally elevated in the peritoneal cavity in endometriosis and might play a role in disease pathogenesis and its associated inflammatory processes. Furthermore, the results show that TFF3 is regulated through the menstrual cycle. PMID: 27330011
involvement of TFF3 in the pathogenesis of rhonchopathy and OSA PMID: 29028798
Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease.( PMID: 28622393
expression reduces growth, viability, and tumorigenicity of retinoblastoma cell oines PMID: 27626280
The expression of TFF3 and TWIST1 might be associated with the survival of patients with colorectal cancer (CRC) after curative resection and might be pivotal predictors of disease progression. TFF3 may be correlated to the invasiveness of CRC. PMID: 28104986
Serum TFF-3 is not a convenient and reliable surrogate marker of mucosal healing during therapy with TNF-alpha antagonists in Crohn's disease. PMID: 28104989
TFF3 levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
TFF3 protein knockout can facilitate apoptosis of human pituitary adenoma HP75 cells via mitochondrial pathway PMID: 26823779
Data show that trefoil factor-3 (TFF3) secreted from mammary carcinoma cells promotes de novo angiogenesis via interleukin-8 (IL-8)/IL-8 receptor CXCR2. PMID: 26559818
Dimers CXCR4 and CXCR7 are involved in TFF3-dependent activation of cell migration, but not cell proliferation. The ERK1/2 pathway is activated in the process, but not influenced by CXCR4 or CXCR7. PMID: 26780310
TFF3 expression is associated with response to endocrine therapy, and outperforms oestrogen receptor, progesterone receptor and TFF1 as an independent biomarker, possibly because it mediates the malign effects of oestrogen on invasion and metastasis PMID: 25900183
the results provide a mechanistic explanation for ITF-mediated protection of gastric epithelial mucosa cells, suggesting that activation of the ERK1/2 signaling pathway may provide a new therapeutic strategy for repairing gastric injury. PMID: 25776570
primary outcome measures were to evaluate the safety, acceptability, and accuracy of the Cytosponge-TFF3 test compared with endoscopy and biopsy. In all, 1,042 (93.9%) patients successfully swallowed the Cytosponge, and there were no serious side effects PMID: 25634542
TFF3 is not only a useful biomarker for a long-term surgical result in CRC patient, but also may be a risk factor of early recurrence. PMID: 25405728
Associations discovered between trefoil factor 3 gene variants and idiopathic recurrent spontaneous abortion. PMID: 25444508
TFF3 expression predicts metastasis and poor survival outcome of patients with mammary carcinoma. PMID: 25266665
TFF3 expression may be absent in the early stages of Barrett's esophagus and its expression may represent more established columnar epithelium. PMID: 25424203
This study suggests that TFF3 is an independent indicator for survival in GC, while HER2 is not associated with the outcome. Patients with HER2-negative/TFF3-negative GC have the best outcome. PMID: 25514872
High TFF3 expression is associated with reduces sensitivity to ionising radiation in prostate carcinoma. PMID: 25748388
Study supports the notion that ITF can promote cell proliferation, migration and preserve epithelium integrity. PMID: 24990304
LRP2 sequencing reveals multiple rare variants associated with urinary trefoil factor-3 PMID: 24876117
Serum concentrations of TFF3 is significantly higher in chronic kidney disease. PMID: 24282531
TFF3 expression at both mRNA and protein levels was significantly downregulated by ErbB-2 silencing in breast cancer cell lines PMID: 24164280
A significantly higher trefoil factor 3 expression in luminal epithelial cells was detected in women with unexplained infertility PMID: 24228773
TFF3 point mutations seem to be a rare event in colorectal carcinogenesis; TFF3 expression may play a role in promoting lymph node metastases of CRCs. PMID: 24125969
TFF3 is an independent indicator for overall survival in gastric cancer. PMID: 23822993
In serum TFFs and pepsinogen tests, only serum TFF3s were not significantly affected by H. pylori eradication, suggesting that serum TFF3 could be a stable biomarker of gastric cancer risk even after H.pylori eradication PMID: 22907485
TFF3 hypermethylation is associated with triple-negative breast cancers. PMID: 24045095
interaction between DMBT1(gp340) and trefoil TFFs proteins was investigated using an ELISA assay. DMBT1(gp340) bound to solid-phase bound recombinant dimeric TFF3 in a calcium dependent manner PMID: 23691218
ER+ /PR+ /TFF3+ /IMP3- immunoprofile distinguishes endometrioid from serous and clear cell carcinomas of the endometrium. PMID: 23570281
Using a Caco-2 cell model the study showed that TLR2 and TFF3 inhibit the IL-1beta induced increase in permeability and release of proinflammatory cytokines, and that this effect is mediated by activation of PI3K/Akt signaling. PMID: 24051092
Distinct ERG and TFF3 protein was expressed in 45% (43/96) and 36% (35/96) of prostate cancers, respectively. PMID: 23856515
Our study suggests that Twist, as an important potential downstream effector, plays a key role in TFF3-modulated metastasis in gastric cancer and can be a promising therapeutic target against intestinal-type gastric cancer. PMID: 23845905
TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
TFF3 seems to be a novel pathway in the pathogenesis of type I endometrial carcinomas. PMID: 23578537
Upregulation of TFF3 after chemoradiotherapy for rectal cancer is associated with a higher risk of relapse. The physiological role of TFF3 in restoring the mucosa during CRT could be interfering with treatment efficacy. PMID: 22516806
Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration. PMID: 22572548
TFF3 may contribute to ocular surface wound healing after Amniotic membrane transplantation, but its production by AM is not further inducible by proinflammatory stimuli. PMID: 22476621
Report TFF3 expression in normal/diseased pancreas. PMID: 22286382
Differential expression of MUC5AC involves crosstalk between interleukin-1beta and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. PMID: 22389405

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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