Recombinant Human TGFB3 Protein

Name: Recombinant Human TGFB3 Protein
Brand: Beta LifeScience
SKU: BL-2475PS
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Background	Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Description	TGF-b 3 Human Recombinant expressed in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 112a.a. and having a molecular weight of 25kDa. The TGF-b 3 is purified by standard chromatographic techniques.
Source	CHO
AA Sequence	The sequence of the first five N-terminalaa was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Purity	>97.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Bioactivity	The biological activity was determined using cell toxicity assay via HT-2 cells. The ED50 was found to be < 0.05ng/ml, corresponding to a specific activity of 20,000,000IU/mg.
Formulation	The protein solution contains 20% Ethanol and 0.1% Acetic acid.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	TGF-beta 3 although stable at 4°C. for 3 weeks, should be stored at -20°C to -70°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.