Recombinant Human TSLP Protein (R127A, R130A, C-10His)
Beta LifeScience
SKU/CAT #: BL-2518NP
BL-2518NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Recombinant Human TSLP Protein (R127A, R130A, C-10His)
Beta LifeScience
SKU/CAT #: BL-2518NP
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159(R127A, R130A) is expressed with a 10His tag at the C-terminus. |
| Accession | Q969D9 |
| Synonym | Thymic stromal lymphopoietin; TSLP |
| Gene Background | Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7. The human TSLP protein comprises a 28 amino acids (aa) signal sequence and 131 aa mature region. Human TSLP has two isoforms lfTSLP and sfTSLP produced by alternative splicing . lfTSLP is expressed in a number of tissues including heart, liver and prostate, and sfTSLP (63aa) is predominantly expressed in keratinocytes of oral mucosa, skin and in salivary glands. In aa sequence level, Human TSLP displays about 43% identity with mouse TSLP.TSLP is a cytokine that functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells.TSLP has proliferative effects on the myeloid cell line and may initiate asthma or atopic dermatitis responses by directly activating mast cells . TSLP signals cells via the interleukin-7 receptor-α chain (IL-7Rα),shared with IL-7, together with the TSLP receptor (TSLPR) subunit. Recent studies indicate that TSLP and its receptor are novel therapeutic targets for rheumatoid arthritis,for increased intraarticular TSLP concentrations in patients has caused chemotaxis and activation of arthritogenic T cells. |
| Molecular Mass | 16.1 KDa |
| Apmol Mass | 20-27 KDa, reducing conditions |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 4% Sucrose, 4% Mannitol, 0.02% Tween 80 (w/v), pH6.0. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Biologically active. Please contact us to obtain bioactivity data. |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Usage | For Research Use Only |
Target Details
| Target Function | Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.; May act as an antimicrobial peptide in the oral cavity and on the skin. |
| Subcellular Location | Secreted. |
| Database References | |
| Tissue Specificity | Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. |
Gene Functions References
- TSLP receptor allosterically activates TSLP to potentiate the recruitment of the shared interleukin 7 receptor alpha-chain (IL-7Ralpha) by leveraging the flexibility, conformational heterogeneity and electrostatics of the cytokine. PMID: 28368013
- Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells. PMID: 30011850
- presence of epithelium-derived TSLP in the eustachian tubes plays important role in the onset of eosinophilic otitis media PMID: 29272985
- a new mechanism of T cell stimulation in human skin was identified, demonstrating the direct induction of T cell migration by TSLP in the absence of DCs. PMID: 28377574
- the secretion of TSLP by keratinocytes is induced by the HIV-1 viral synapse in a miR-375 controlled manner PMID: 28443609
- production increased at skin sites of ovalbumin sensitization PMID: 27992078
- Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels. PMID: 26948075
- short and long thymic stromal lymphopoietin isoforms have roles in house dust mite-induced asthmatic airway epithelial barrier disruption PMID: 27996052
- DeltaNp73 was abundantly expressed in the atopic dermatitis epidermis and increased the release of TSLP via NF-kappaB activation. PMID: 28655470
- this study shows that long form of thymic stromal lymphopoietin of keratinocytes is induced by protein allergens PMID: 28720058
- we found that activation of the protease-activated receptor 1 (PAR1) induced secretion of TSLP by the corneal stromal cells... we proposed that TSLP might function as the link between increased protease activity and inflammatory responses or itch sensation in the corneas PMID: 28631887
- TSLP-HOTAIR axis also plays a protective role in low-density lipoprotein (ox-LDL)-induced Endothelial cells' injury. PMID: 28615347
- The aim of this study was to determine the influence of human rhinovirus on the genes involved in airway remodeling and to examine the impact of TSLP and contribution of oxidative stress on airway remodeling in the context of HRV infection. PMID: 28545810
- TSLP-induced phosphorylation of l-plastin is increased in atopic dermatitis and increases eosinophil migration. PMID: 27304220
- a hit expansion exercise yielded additional active compounds that could be amenable to further optimization, providing an opportunity to dissociate TSLP inhibition from other non-desired activities. This study illustrates the potential of phenotypic drug discovery to complement target based approaches by providing new chemistry and biology leads PMID: 29320511
- study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
- lactobacilli from the omnivorous group and all bifidobacteria significantly down-regulated IL-8. Notably, both genera also lowered the TSLP expression in stimulated Caco-2 cells, regardless of the diet regimen. PMID: 27863334
- this review focuses on recent advances on the effects of TSLP on obstetrical and gynecological diseases, including pregnancy failure, endometriosis, and cervical cancer PMID: 27976427
- The increase in TSLP and TNF-alpha level observed in IS fluid was found to correlate with disease severity. The increased TSLP production from asthma sputum cells was abrogated by the addition of rIL-37. Regulation of TSLP pathway may be a therapeutic approach for asthma. PMID: 27528425
- House dust mite sublingual immunotherapy downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe allergic rhinitis. PMID: 27012942
- The results suggest that IL-1beta may be an early key mediator for the acquisition of an Atopic dermatitis (AD) phenotype through induction of thymic stromal lymphopoietin (TSLP) and alteration of the epidermal homeostasis. PMID: 28191908
- House dust mite exposure increases the expression of TSLP through PI3K/Akt signaling pathway in human bronchial epithelial 16HBE cells. PMID: 28400057
- Posttranslational modifications control the functional activity of TSLP in humans and overproduction of TSLP may be a key trigger for the amplification of type 2 inflammation in diseases PMID: 27744031
- Study provides evidence for the hypothesis that TSLP could reflect the histological severity of lumbar disc degeneration (LDD), and TSLP-siRNA and TSLPR-siRNA could inhibit apoptosis of nucleus pulposus cells. The evident information obtained from the investigation could lead the way for new therapeutic approaches regarding LDD treatment. PMID: 28746197
- periostin-mediated TSLP production by keratinocytes directly stimulates cutaneous T-cell lymphoma (CTCL) tumor cell growth in addition to inducing a Th2-dominant tumor environment in CTCL. PMID: 27634769
- this study shows that TSLP may be involved in the pathogenesis of asthma and rhinitis; dust mite and mugwort allergy could increase it significantly PMID: 28303765
- Serum TSLP levels were significantly increased in systemic sclerosis patients compared to healthy donors, and were associated with a higher frequency of vasculopathy. The proportion of TSLP-positive dermal cells was increased in the skin of SSc patients compared with healthy donors, and was correlated with fibrosis. PMID: 27429171
- this study shows that NOD2 is an asthma-related factor that can promote cell proliferation and inflammatory response by mediated expression of TSLP in human airway smooth muscle cells PMID: 27889082
- the effects of TSLP on myeloid cells are crucial in reducing the multiple organ failure that is associated with systemic inflammation. PMID: 26934097
- interactions between TSLP in follicular dendritic cells and IgA production in tonsils may be an important mechanism contributing to the pathogenesis of Immunoglobulin A (IgA) nephropathy. PMID: 27187742
- Study demonstrated that naturally occurring severe infections by the most common respiratory viruses in hospitalized infants induce nasal airway secretion of TSLP, IL-33, and periostin when compared with healthy controls PMID: 28471975
- Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
- A possible role of TSLP in immune homeostasis in HIV infection:higher plasma TSLP was found in individuals with primary HIV infection compared to both chronic HIV infection and healthy controls. PMID: 27769179
- The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40) that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4). Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1) in a human cell line (HEK293) expressing NHLH1 resulted in lower TSLP expression PMID: 27149122
- TSLP level was significantly higher in asthmatic than in nonasthmatic children. TSLP level was significantly different between allergic asthmatic and nonallergic nonasthmatic groups. PMID: 26999524
- While serum TSLP levels were unaffected by concomitant allergies and atopic comorbidities, serum levels of IL-31, IL-33 and sST2 were affected to a small extent. We found a positive correlation between TSLP, IL-31 and IL-33, and an inverse relationship between IL-33 and sST2 PMID: 27152943
- Results showed that TSLP promoted the production of alpha-SMA and collagen I (P<0.001), suggesting that it can accelerate MRC-5 cell fibrosis. PMID: 27385084
- TSLP is a key mediator in the pathogenesis of inflammatory bowel diseases and that further studies are needed to evaluate its role. [review] PMID: 27697608
- Letter: report decreased salivary gland TSLP expression in primary Sjogren's syndrome. PMID: 27494755
- A novel mouse xenograft model to study the role of TSLP-induced CRLF2 signals in normal and malignant human B lymphopoiesis has been developed. PMID: 26611474
- Heat stress-induced HSPs can significantly reduce the production and secretion of TSLP from keratinocytes cultured under Th2 environment. PMID: 26419317
- TSLP expression in the skin is mediated via RARgamma-RXR pathways. PMID: 26531761
- Epidermal thymic stromal lymphopoietin predicts the development of atopic dermatitis during infancy. PMID: 26879860
- study shows that specific food antigens can trigger innate immune mediated esophageal TSLP secretion PMID: 26992000
- production by monocyte-derived dendritic cells requires the integration of signals from dectin-1, the IL-1 receptor, and endoplasmic reticulum stress response stress signaling pathways PMID: 26573878
- The association of TSLP polymorphism rs2289278 with atopic dermatitis (AD) was stronger in children with allergic sensitization than in children without atopy, and TSLP polymorphisms also increased the risk of asthma in children with AD PMID: 26712523
- The positive feedback loop between TSLP, IL-33 and their receptors, and Th2 cytokines may facilitate Th2-skewed inflammation in eosinophilic CRSwNP. PMID: 26095319
- results show that rs1837253 polymorphism may be directly involved in the regulation of TSLP secretion. PMID: 25515628
- Upon TSLP stimulation, the cells with the highest TSLPR expression level showed enhanced proliferation and JAK/STAT-mediated gene regulation in a dose-dependent manner PMID: 26652578
- Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent PMID: 25962901
