Recombinant Influenza A Virus Nucleoprotein (NP) Protein (His-B2M)
Beta LifeScience
SKU/CAT #: BLC-03658P
Greater than 90% as determined by SDS-PAGE.
Recombinant Influenza A Virus Nucleoprotein (NP) Protein (His-B2M)
Beta LifeScience
SKU/CAT #: BLC-03658P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Influenza A Virus Nucleoprotein (NP) Protein (His-B2M) is produced by our E.coli expression system. This is a full length protein. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprotkb | P03466 |
| Target Symbol | NP |
| Synonyms | NP; Nucleoprotein; Nucleocapsid protein; Protein N |
| Species | Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) |
| Expression System | E.coli |
| Tag | N-6His-B2M |
| Target Protein Sequence | MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDN |
| Expression Range | 1-498aa |
| Protein Length | Full Length |
| Mol. Weight | 70.2kDa |
| Research Area | Microbiology |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. |
| Subcellular Location | Virion. Host nucleus. |
| Protein Families | Influenza viruses nucleoprotein family |
| Database References | KEGG: vg:956531 |
Gene Functions References
- Taking these observations together, TRIM41 is a constitutively expressed intrinsic influenza A virus restriction factor that targets viral NP for ubiquitination and protein degradation. PMID: 29899090
- Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein has been reported. PMID: 26456934
- Immunoprecipitation studies revealed influenza A virus NS1 and NP as direct interaction partners of DDX3. PMID: 26792746
- Residue 88 of NP interacts with polymerase PB2 and contributes to influenza A virus replication. PMID: 25043584
- HMGB1 protein binds to influenza virus nucleoprotein and promotes viral replication. PMID: 22696656
- These results indicate that the PB2, PA, NP, and M segments play a more important role than the remaining four viral RNAs during the genome-packaging process. PMID: 22532680
- Data suggest that the flexibility of loops 1 and 2 is required for RNA sampling and binding which likely involve conformational change(s) of the nucleoprotein. PMID: 22272272
- These findings demonstrate that replication of the virus genome is followed by its encapsidation by NP in collaboration with its chaperone UAP56. PMID: 21507964
- Because the authors showed that NP lacking RNA-binding activity binds directly to the viral polymerase, it is likely that a direct interaction between NP and the viral polymerase results in a modification of the polymerase in favor of unprimed initiation. PMID: 18945782
- The authors identified a late-acting defect in NP that, separate from its function in RNA synthesis, indicates a role for the polypeptide in virion assembly, most likely involving M1 as a partner. PMID: 18987140
- respective conserved amino acids in NP may thus be critical for the assembly and/or incorporation of sets of eight viral-RNA segments PMID: 19225007
