Recombinant Mouse BD14 Protein

Name: Recombinant Mouse BD14 Protein
Brand: Beta LifeScience
SKU: BL-0388PS
Availability: InStock

Collections: Cytokines and growth factors, Other cytokines, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Mouse
Synonym	Beta-defensin 14, BD-14, mBD-14, Defensin, beta 14, Defb14.
Background	Alpha and Beta Defensins are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi and enveloped viruses. These 2-6kDa proteins have vital roles in innate immune system. Mammalian Defensins are classified into alpha, beta and theta categories, based on their size and pattern of disulfide bonding. Beta-Defensins contain a six-cysteine motif which forms 3 intra-molecular disulfide bonds. Since beta-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. In addition, they can affect the stability of the membrane.
Description	Beta Defensin-14 Mouse Recombinant expressed in E.coli is a single, non-glycosylated, polypeptide chain containing 45a.a. and having a molecular weight of 5.2kDa.The BD14 is purified by unique purification methods.
Source	E.coli
AA Sequence	FLPKTLRKFF CRIRGGRCAV LNCLGKEEQI GRCSNSGRKC CRKKK.
Purity	>96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	BD-14 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Lyophilized Mouse BD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Target Details

Target Function	Has antibacterial activity.
Subcellular Location	Secreted.
Protein Families	Beta-defensin family
Database References	KEGG: mmu:244332 STRING: 10090.ENSMUSP00000062781 UniGene: Mm.358923

Gene Functions References

this work demonstrates that mouse beta-defensin-14 can promote the maturation of dendritic cells in which TLR-4 is possibly involved PMID: 29253819
This study indicated that the potential antibacterial role of increased MBD-14 in the osteomyelitis mouse model. PMID: 24489798
BD14 expression by tumor-infiltrating host cells results in chemoattraction of CCR6-positive B220-positive lymphocytes, which in turn initiates a proangiogenic pathway leading to enhanced angiogenesis and organized tumor tissue development. PMID: 22504651
Murine beta-defensin-14, like ultraviolet radiation (UVR), can shift surface marker expression of CD4+CD25- T cells toward a regulatory (Treg) phenotype but does not appear to play a major role in UVR-induced immunosuppression. PMID: 22174455
beta-defensin 4 and 14 contribute to the innate and adaptive immune response in their role as chemoattractants PMID: 20483750
both hBD3 and mBD14 were chemotactic for freshly isolated mouse resident peritoneal cells. Thus, mBD14, based on structural and functional similarities, appears to be an orthologue of hBD3. PMID: 18167348
chemoattractant and antimicrobial activities of beta-defensins can be separated, and both of these functions are independent of intramolecular disulfide bonds PMID: 18180295

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.