Recombinant Mouse C-C Chemokine Receptor Type 5 (CCR5) Protein (His/Tag-Free)

Name: Recombinant Mouse C-C Chemokine Receptor Type 5 (CCR5) Protein (His/Tag-Free)
Brand: Beta LifeScience
SKU: BLC-08213P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Ccr5.

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Product Overview

Description	Recombinant Mouse C-C Chemokine Receptor Type 5 (CCR5) Protein (His/Tag-Free) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P51682
Target Symbol	CCR5
Synonyms	Ccr5; Cmkbr5C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; MIP-1 alpha receptor; CD antigen CD195
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	N-His/Tag-Free
Protein Length	Partial
Mol. Weight	12.6kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	KEGG: mmu:12774 STRING: 10090.ENSMUSP00000107069 UniGene: Mm.14302

Gene Functions References

Data provides evidence that CCR5 has an essential role in bone-destructive conditions through the functional regulation of osteoclasts. PMID: 29263385
Blockade of CCR5-mediated myeloid derived suppressor cell accumulation enhances anti-PD1 efficacy in gastric cancer PMID: 29303012
the interaction between CCR5 and its ligands promotes the proliferation of CCR5(+) polymorphonuclear-myeloid-derived suppressor cells at the bone marrow PMID: 29166611
This study suggested a potential neuroprotection in the absence of CCR5 receptor during global brain ischemia and reperfusion injury. PMID: 28294064
Studied the effects of CCL5-CCR5 interactions in breast cancer metabolism, and findings suggest that CCL5-CCR5 interactions in the tumor microenvironment modulate metabolic events during tumor onset to promote tumorigenesis. PMID: 29216863
Loss of CCR5 is associated with astrogliosis, amyloid-beta deposit and impaired memory function. PMID: 26910914
These findings suggest that CCR5 is likely participating in demyelination in the spinal cord in experimental autoimmune encephalomyelitis PMID: 26985768
These results demonstrate that CCR5 plays an important role in neuroplasticity, learning and memory, and indicate that CCR5 has a role in the cognitive deficits caused by HIV. PMID: 27996938
The Ccr5 is crucial in directing T cells toward the Langat virus -infected brain, as well as in suppressing neutrophil-mediated inflammation within the Central Nervous System. PMID: 27183602
This study showed that CCR5 ablation exacerbated Japanese encephalitis without altering viral burden in the extraneural and CNS tissues, as manifested by increased CNS infiltration of Ly-6C(hi) monocytes and Ly-6G(hi) granulocytes. PMID: 27439902
this review discusses the role of CCR5 in recruitment and activation of myeloid-derived suppressor cells in melanoma PMID: 28382399
These results suggested that CCR5 signaling is involved in embryo loss in Toxoplasma gondii infection during early pregnancy and that apoptosis is associated with embryo loss rather than direct damage to the fetoplacental tissues. PMID: 28630065
The upregulation of CCR5 on the surface of the CD8(+) T cells increases the number of contacts with Ag-bearing dendritic cells, which ultimately results in increased CD8(+) T cell response to Ag rechallenge. PMID: 26994221
CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
Cytokine-induced killer cells interact with tumor lysate-pulsed dendritic cells via CCR5 signaling. PMID: 27216980
this study shows that diosgenin-mediated anti-allergic effects are associated with increased number of Foxp3+ Treg cells expressing CCR5 PMID: 27886644
CCR5 deficiency increased the production of TNF-alpha following LPS treatment through increased activation of the p38 pathway in the kidney, resulting in renal apoptosis and leukocyte infiltration and led to exacerbation of LPS-induced acute kidney injury. PMID: 26055553
In West Nile virus infection of the central nervous system, CCR5 activity is required to limit viral burden in the cerebral cortex. PMID: 26667390
Data show that the activation of mammalian target of rapamycin complex mTORC1 during encephalomyocarditis virus infection is chemokine (C-C) receptor 5-dependent and promotes the translation of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. PMID: 26408666
CCL5-induced endothelial progenitor cell migration was increased by overexpression of CCR5 and that increase was abolished by addition of CCL5 antibody, suggesting CCL5/CCR5 interaction is involved in chemotactic effects of endothelial progenitor cells. PMID: 25889019
These findings show that migration and activation of immune cells via CCR5 is required for controlling N. caninum parasites during the early phase of the infection. PMID: 25558986
our results suggest that the detrimental effects of C5a in this model are partly mediated through CCR5 activation downstream of C5aR1, which may be evaluated for potential therapeutic exploitation in ALI/ARDS. PMID: 25999468
CCR5 blockade promotes M2 macrophage activation and improves locomotor activity after spinal cord injury in mice. PMID: 25212047
CCR5 KO mice show less cartilage degeneration but no change in bone or synovial response to medial meniscal destabilization. PMID: 25498590
Results indicate that CCR5 deficiency modifies the nigrostriatal dopaminergic neuronal system and bidirectional interaction between neurons and glial cells via CCR5 might be important for dopaminergic neuronal survival PMID: 22922220
These data bring new insights on the association between viral infections and the chemokine receptor CCR5. PMID: 25939314
CCR5 is essential to the control of T. gondii infection and to maintain the metabolic, hepatic and intestinal integrity. PMID: 25119429
Mycobacterium infection significantly increased CCR5 expression in macrophages there by facilitating the activation of its downstream signaling. These events culminated in up-regulation of the immunosuppressive cytokine IL-10 production PMID: 24695099
These results reveal novel alloreactive CD8 T cell specificities in CCR5-deficient recipients of single class II MHC renal allografts that mediate rejection of the allografts. PMID: 25172484
This study provides evidence for an indirect pathologic role of CCR5 and a novel protective effect of LCN2 in combination with inhibition of CCR5 in HIV-associated brain injury. PMID: 25031461
mediates neutrophil recruitment in acute lung injury PMID: 23860188
CCL3-CCR5-mediated fibroblast accumulation may be required, in addition to leukocyte infiltration, to induce full-blown colitis-associated carcinogenesis. PMID: 24510316
this study for the first time demonstrates the importance of TLR2/CCR5 crosstalk as a significant determinant of Leishmania donovani entry in host macrophages. PMID: 24617012
Inflammation-induced hepatocellular carcinoma is dependent on CCR5 in mice. PMID: 23526353
absence of CCR5 delays the resolution of inflammatory responses triggered by single-walled carbon nanotubes PMID: 22438032
The CCR5/MIP-1alpha axis may contribute to migration of infected cells to the brain and consequently affect the pathogenesis during Rocio virus infection. PMID: 24080631
These findings suggest that whereas CCR5 plays a minor role in regulating immune cell infiltration and inflammation in metabolic tissues, deficiency of CCR5 impairs systemic glucose tolerance as well as adipose tissue and muscle insulin signaling. PMID: 23941876
Pain responses of CCR5 knockout mice to chemical or inflammation stimuli are milder than those of CCR5 wild-type mice. PMID: 23147416
These results suggest that the absence of CCR5 may boost the immune response with a high neutrophil recruitment which most likely helps in viral clearance. PMID: 23391218
ata indicate an expansion of CXCR3(+) and CCR5(+) T cells was observed in the tumor. PMID: 23326300
the critical mechanism underlying enhanced effect of CCR5-transducedneural stem cells on experimental autoimmune encephalomyelitis is the early migration of chemokine receptor-transduced NSCs into the inflamed foci. PMID: 22526024
CCR5 deficiency suppressed lung tumor development through the inhibition of nuclear factor-kappaB/STAT3 pathways and the downregulation of MCP-1 in the carcinogen-induced lung tumor model. PMID: 22907530
the immune responses against JEV in mice lacking expression of the chemokine receptor CCR5 PMID: 23028638
identification of the human immunodeficiency virus (HIV) co-receptor CCR5 as a cellular determinant required for cytotoxic targeting of subsets of myeloid cells and T lymphocytes by the S. aureus leukotoxin ED (LukED) PMID: 23235831
These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
leukostasis in early diabetic retinopathy involves activated CCR5(+)CD11b(+) myeloid cells (presumed monocytes). PMID: 22677420
analysis of transmembrane protein aptamers that inhibit CCR5 expression and HIV coreceptor function PMID: 22811524
Potent in vivo suppression ability of CD103-positive regulatory T cells (Treg) is not due to stronger suppression ability per cell but due to their tissue migration ability through CCR5 expression. PMID: 22664873
The results showed that CCR5 deficiency caused apoptotic cell death of melanoma through inhibition of NF-kappaB and upregulation of IL-1Ra. PMID: 22567084
It was shown that CCR5 plays a critical role in adipose tissue macrophage recruitment and polarization and subsequent development of insulin resistance. PMID: 22474027

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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