Recombinant Mouse C-C Motif Chemokine 5 Protein (CCL5), Active

Name: Recombinant Mouse C-C Motif Chemokine 5 Protein (CCL5), Active
Brand: Beta LifeScience
SKU: BLC-05479P
Availability: InStock

Collections: Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse C-C Motif Chemokine 5 Protein (CCL5), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T lymphocytes is in a concentration range of 1.0-10 ng/ml.
Uniprotkb	P30882
Target Symbol	CCL5
Synonyms	Ccl5; Scya5C-C motif chemokine 5; MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
Expression Range	24-91aa
Protein Length	Full Length of Mature Protein
Mol. Weight	7.9 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered concentrated solution in 30 Acetonitrile and 0.1 TFA
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: mmu:20304 STRING: 10090.ENSMUSP00000039600 UniGene: Mm.284248
Tissue Specificity	T-cell and macrophage specific.

Gene Functions References

the results show that Broadleaf Mahonia is a novel effective antiinflammatory herb in vitro and ex vivo, and that CCL5 may be closely associated with Granulomatous lobular mastitispathogenesis PMID: 29138800
miR-155 enhances venous neointima formation through the autocrine and paracrine effects of smooth muscle-like cell-derived RANTES in a nuclear factor kappaB-dependent manner in arteriovenous shunts. PMID: 29477204
Taken together, our data suggest that CCR5 regulates insulin signaling in hypothalamus which contributes to systemic insulin sensitivity and glucose metabolism. PMID: 27898058
identifies the essential role of the chemoattractive cytokine CCL5 for liver disease progression and especially hepatocellular carcinoma development PMID: 28011329
Breast cancer cell CCL5 mediates bone marrow independent angiogenesis via paracrine signaling. PMID: 27863423
Studied the effects of CCL5-CCR5 interactions in breast cancer metabolism, and findings suggest that CCL5-CCR5 interactions in the tumor microenvironment modulate metabolic events during tumor onset to promote tumorigenesis. PMID: 29216863
RANTES levels were associated with TMS measures of cortical synaptic excitability, but not with long-term potentiation (LTP)-like plasticity. PMID: 26733422
study found that the inflammatory chemokine CCL5 is mostly retained (75%) during the resolution of zymosan A peritonitis in mice; CCL5 exerts a novel proresolving role on macrophages when acting in concert with apoptotic PMN-expressed D6. PMID: 28674178
A robust up-regulation of RANTES within the brain was seen in a mouse model of tick-borne encephalitis. PMID: 27576490
This study showed that RANTES is important in the regulation of vascular dysfunction through modulation of perivascular inflammation. PMID: 26873938
Blocking antibodies against RANTES and eotaxin reduced the infiltration of CD4(+) and CD8(+) T cells into the nigra, attenuated nigral expression of proinflammatory molecules, and suppressed nigral activation of glial cells. These findings paralleled dopaminergic neuronal protection, normalized striatal neurotransmitters, and improved motor functions in MPTP-intoxicated mice. PMID: 27226559
CCL5 deficiency resulted in reduced neointima formation after carotid artery injury and thrombosis. PMID: 27337700
RANTES produced by renal tubular cells act as a key chemokine in acute kidney injury and HIF-1alpha regulated LncRNA-PRINS might be involved in RANTES production. PMID: 26725683
Neutralization of circulating RANTES decreased liver neutrophilic infiltration PMID: 26799785
CCL5 signaling through CCR5 may increase platelet counts during physiological stress. PMID: 26647394
An intriguing finding was that S1P induced c-Fos-inhibited CCL5 directly and also indirectly through inhibition of the IFN-beta amplification loop PMID: 26246404
platelet-derived CCL5 is an important link between platelet activation and neutrophil recruitment in acute colitis PMID: 26089223
Nuclear FAK is associated with chromatin and exists in complex with transcription factors and their upstream regulators that control Ccl5 expression. Furthermore, FAK's immuno-modulatory nuclear activities may be specific to cancerous squamous epithelial cells, as normal keratinocytes do not have nuclear FAK. PMID: 26406376
PRDX6 promotes lung tumor development via its mediated and CCL5-associated activation of the JAK2/STAT3 pathway. PMID: 25582888
High levels of CCL5 expression in mouse and human cell lines might accelerate diffuse large Bcell lymphoma formation in diabetes mellitus. PMID: 24938715
IL-17A contributes significantly to the pathogenesis of renal fibrosis by regulating RANTES-mediated inflammatory cell infiltration PMID: 25158055
decreased CCL5 mRNA expression was observed in kidneys of lupus-prone mice with reduced Fli-1 expression. CCL5 protein expression was significantly decreased in endothelial cells transfected with Fli-1-specific small interfering RNA. PMID: 25098295
We evaluated the mRNA expression of angiogenic and migration factors in MSC and found the expression of CCL5 mRNA was higher in AT-MSC than in BM-MSC or DT-MSC. PMID: 24171667
These results show that IL-32gamma effectively promotes migration of activated T cells via CCL5 production in DCs. PMID: 24882804
TLR4, rather than TLR2, regulates wound healing through TGF-beta and CCL5 expression PMID: 24252748
HIV-1 Tat-mediated induction of CCL5 in astrocytes involves NF-kappaB, AP-1, C/EBPalpha and C/EBPgamma transcription factors and JAK, PI3K/Akt and p38 MAPK signaling pathways PMID: 24244375
WT1 regulates the expression levels of Cxcl10 and Ccl5 in epicardial cells directly and indirectly through increasing the levels of IRF7. PMID: 23900076
this study identifies a new role for SARM in CCL5 expression in macrophages. PMID: 24711619
we found that IRF1 was essential for IL-1-induced expression of the chemokines CXCL10 and CCL5 PMID: 24464131
CCL5 is a target gene for AhR, and might be associated with the pathology of dioxin exposure. PMID: 23810773
These data provide in vivo evidence supporting the previously suggested role of Ccl5 in bone remodeling. PMID: 23553711
Taken together the data presented strongly suggest that 4T1 mammary tumor cell invasion in vitro is promoted by D1 mesenchymal stem cell secretions, especially the chemokines CCL5 and CCL9. PMID: 23722213
Sphingosine kinase 1 regulates tumor necrosis factor-mediated RANTES induction through p38 mitogen-activated protein kinase but independently of nuclear factor kappaB activation. PMID: 23935096
Study uncovers a novel, chemokine-independent activity of the hematopoietically derived CCL5 that promotes mammary tumor progression via generating MDSCs in the bone marrow in cooperation with tumor-derived colony-stimulating factors. PMID: 23266888
Alloreactive memory CD4(+) T cells contribute to increased expression and secretion of RANTES, and to the Tm and other inflammatory cells migration into the graft. PMID: 23498790
Data indicate that enhanced tumorigenesis is dependent on microbiota-induced chemokine CCL5-driven inflammation, which in turn promotes epithelial activation of the IL-6 pathway, leading to cancer formation. PMID: 23696660
Peptide inhibition of CXCL4-CCL5 interactions may represent a viable translational strategy to limit progression of abdominal aortic aneurysms. PMID: 23288157
Attenuated behavioral hypersensitivity in CCL5-deficient mice observed in the current study could be a result of decreased macrophage infiltration, mobilization, and functional ability at injured sites in neuropathic pain. PMID: 22494919
Chinese herbal antidotes A, H and C in combination can significantly inhibit the RANTES expression in serum and renal tissue of MRL/lpr mice. PMID: 21302497
findings show that effector T cells cannot accumulate within the decidua, the specialized stromal tissue encapsulating the fetus and placenta; impaired accumulation was in part attributable to the epigenetic silencing of key T cell-attracting inflammatory chemokine genes in decidual stromal cells PMID: 22679098
Fbw7 gamma - mediated ubiquitination of KLF13 prevents RANTES expression in resting human but not murine T lymphocytes. PMID: 22797700
shikonin can effectively enhance anti-tumor potency of a gene-based cancer vaccine via the induction of RANTES expression at the skin immunization site. PMID: 22494696
CCL5 functions within the glioblastoma microenvironment through CCR1 and CCR5 in a redundant manner. PMID: 22425022
IL-15 can signal via IL-15Ralpha, JNK, and NF-kappaB to drive RANTES production by myeloid cells. PMID: 22447977
These data confirm CCL5 is an essential factor for optimal pneumococcal adaptive immunity and show CD4(+) T cell responses to PspA(199-246) are largely resistant to CCL5 deficiency. PMID: 22178100
the involvement of CCL5 in the pathogenesis of colorectal carcinoma PMID: 22205974
Data identify the CCL5/CCR5 interaction as a possible novel molecular target for modulation of neovascularization and eventual tissue repair. PMID: 22214846
Interferon alpha/beta control RANTES production during pneumococcal pneumonia in mice. PMID: 22156592
RANTES/CCL5 mediates trafficking and homing of T lymphocytes, monocytes, and other leukocytes, and has been implicated in arterial injury and atherosclerosis. PMID: 21680945
an important role for RANTES in sustaining CD8 T cell responses during a systemic chronic viral infection. PMID: 21814510

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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