Recombinant Mouse C-C Motif Chemokine 7 Protein (CCL7)

Beta LifeScience SKU/CAT #: BLC-05477P

Recombinant Mouse C-C Motif Chemokine 7 Protein (CCL7)

Beta LifeScience SKU/CAT #: BLC-05477P
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Product Overview

Description Recombinant Mouse C-C Motif Chemokine 7 Protein (CCL7) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
Uniprotkb Q03366
Target Symbol CCL7
Synonyms Ccl7; Fic; Mcp3; Scya7C-C motif chemokine 7; Intercrine/chemokine MARC; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Protein FIC; Small-inducible cytokine A7
Species Mus musculus (Mouse)
Expression System E.Coli
Tag Tag-Free
Complete Sequence QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
Expression Range 24-97aa
Protein Length Full Length of Mature Protein
Mol. Weight 8.5 kDa
Research Area Immunology
Form Liquid or Lyophilized powder
Buffer 0.2 μm filtered 2xPBS, pH 7.4, lyophilized
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Target Details

Target Function Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity.
Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database References

Gene Functions References

  1. The suppression of nasal inflammation due of IL-17A deficiency in allergic rhinitis is partly responsible for the regulation of CCL7 secretion and eosinophil infiltration, which may be regulated via the CCL7/CCR3 pathway. PMID: 28046055
  2. Our results demonstrate that CCL7 is required for maximal ovalbumin-induced ocular anaphylaxis, mast cell recruitment in vivo, and maximal FcepsilonRI-mediated mast cell activation in vitro PMID: 27956527
  3. Periprostatic adipocytes drive prostate cancer progression in obesity via CCL7 secretion which stimulates CCR3 expressing tumor cells. PMID: 26756352
  4. TFPI has an anti-proliferative role in TNF-alpha stimulated-VSMCs at least partly by interfering with the MCP-3/CCR2 pathway and then via suppression of the ERK1/2 and PI3K/AKT signaling pathways PMID: 26302185
  5. Our data highlight a pivotal role of CCL7 and IRF-7 in rhinovirus-induced inflammation PMID: 25847975
  6. CCL7 has a dual role in the development of renal tubular interstitial fibrosis, deleterious in early stages but beneficial during later stages PMID: 23872063
  7. These findings suggest that aortic MCP-3 overexpression may contribute to the development of atherosclerosis and hepatic steatosis under atherogenic conditions. PMID: 23462015
  8. An increase in the expression of MCP-3 along with IL-6 histone modification at the MCP-3 promoter promotes pain sensation associated with enhanced interaction between astrocytes and microglia in the spinal cord PMID: 23364351
  9. Electrical stimulation for 1 h significantly upregulates SDF-1 and MCP-3 expression that persists for 24 hours. PMID: 22006493
  10. Direct anal sphincter injury results in higher levels of SDF-1 and MCP-3 expression soon after injury, whereas denervation via pudendal nerve crush results in greater SDF-1 and MCP-3 expression 10 days after injury. PMID: 21706136
  11. Matrix metalloproteinase-2-mediated chemokine cleavage of MCP3 has an important role in cardiac inflammation as a negative feedback mechanism. PMID: 21986287
  12. expression of the chemokine, stromal cell-derived factor-1 (SDF-1) or monocyte chemotactic protein-3 (MCP-3) may enhance homing of osteogenic cells into sites of fracture repair PMID: 21567452
  13. CCL7 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  14. MCP-3 is significantly over-expressed in the urethral tissues of both wild-type and obese mice immediately after any urethral manipulation. Underlying obesity resulted in alterations in response to tissue injury, paralleling the degree of injury. PMID: 20970834
  15. Monocyte chemoattractant protein-3 plays a critical role in mediating oxidative stress-induced neutrophilic airway inflammation and may have relevance in induction of neutrophilia in severe asthma. PMID: 11777981
  16. in study of relevance of chemokine expression to selective migration of t-cells and the disease localization in murine graft-versus-host disease, mcp3 was found to be predominantly expressed in skin and heart, and not spleen and liver. PMID: 12098066
  17. overexpression of MCP-3 in tight-skin mouse skin suggests a novel role for this protein as a fibrotic mediator activating extracellular matrix gene expression in addition to promoting leukocyte trafficking. PMID: 12847692
  18. These results indicate that CCR7-mediated cortex-to-medulla migration of thymocytes is essential for establishing central tolerance rather than for supporting the maturation or export of thymocytes. PMID: 16473829
  19. a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis. PMID: 18453562
  20. Cross-talk between MCP-3 and TGFbeta may be critical in the development of fibrosis. PMID: 19038247
  21. Up-regulated MCP-3 production does not compensate for loss of MCP-1; MCP-3 appears to be a less effective mediator of monocyte recruitment than MCP-1. PMID: 19641140

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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