Recombinant Mouse C-C Motif Chemokine 9 Protein (CCL9), Active

Name: Recombinant Mouse C-C Motif Chemokine 9 Protein (CCL9), Active
Brand: Beta LifeScience
SKU: BLC-05475P
Availability: InStock

Collections: Chemokines and receptors, Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse C-C Motif Chemokine 9 Protein (CCL9), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 95% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
Uniprotkb	P51670
Target Symbol	CCL9
Synonyms	CCF18; Ccl9; CCL9(31-101); CCL9_MOUSE; Chemokine CC motif ligand 9; Macrophage inflammatory protein 1-gamma; Macrophage inflammatory protein-related protein 2; MIP-1-gamma; MRP-2; SCYA10; SCYA9; Small inducible cytokine A9; Small-inducible cytokine A9
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
Expression Range	22-122aa
Protein Length	Full Length of Mature Protein
Mol. Weight	11.6 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: mmu:20308 STRING: 10090.ENSMUSP00000019266 UniGene: Mm.416125
Tissue Specificity	Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

Gene Functions References

CCL9 secreted by splenic macrophages induces a CCR1dependent accumulation of MDSCs. PMID: 30365155
Results show that MIP-1g promotes tumor cell growth in vitro through the activation of NFkB pathway in colorectal tumor cells. PMID: 28108624
we report that chemokine CCL9 was highly induced in Gr-1(+)CD11b(+) immature myeloid cells and in premetastatic lung in tumor-bearing mice PMID: 26483204
Plasmid codelivery of murine CCL19 and CCL28 enhanced Ag-specific systemic and mucosal Ab responses to HIV-1 gp140, augmented balanced Th1/Th2 responses, and increased lymphocyte subsets in various lymphoid tissues. PMID: 23858028
There is protein-DNA interaction between ETV5 and Ccl9, suggesting that ETV5 might be a direct regulator of Ccl9 expression. PMID: 20799334
Results reveal the potential involvement of CCL9 and CCR1 in macrophage and microglial cells by CpG-ODNs and may help understanding the role of the chemokine/chemokine receptor pairs in macrophage/microglia under physiologic and pathologic conditions. PMID: 19883904
MRP-2 may be an important contributing factor in the development of atherosclerosis PMID: 12173416
CCL9 and its receptor CCR1 are the major chemokine and receptor species expressed by osteoclasts, CCL9 has a role in the regulation of bone resorption PMID: 12397598
An important role for CCL9 is demonstrated in the recruitment of CD11b+ dendritic cells to the dome regions of mouse Peyer's patches. PMID: 12960300
MIP-1 gamma plays an important role in the differentiation & survival of osteoclasts, & bone resorption, most likely via an autocrine pathway. PMID: 15265944
Keratinocyte-derived chemokine (KC) and MIP-2 alpha differ in their mediation of neutrophil function (apoptosis and chemotaxis) and contribution to the pathogenesis of lung injury following hemorrhage subsequent to sepsis. PMID: 15961314
BCR/ABL repressed CCL9 expression at the transcriptional level PMID: 17160016
involved in calcium mobilization and chemotaxis PMID: 11500837

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.