Recombinant Mouse C3a Protein
Beta LifeScience
SKU/CAT #: BL-1937NP
BL-1937NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Recombinant Mouse C3a Protein
Beta LifeScience
SKU/CAT #: BL-1937NP
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Mouse Complement Component C3a is produced by our E.coli expression system and the target gene encoding Ser671-Arg748 is expressed. |
| Accession | P01027 |
| Synonym | Complement Component C3a; Anaphylatoxin; C3a |
| Gene Background | Complement is defined as key part of innate immunity and as the first line of defense in the fight against invading pathogens. Complement 3 (C3) is the most abundant component of the complement cascade and the convergent point for all three major complement activation pathways: namely classical, alternative and mannose-binding lectin pathways. Complement activation leads to the formation of the C3 convertase, which cleaves C3 into the key effector molecules, C3a (anaphylatoxin) and C3b (opsonin) which then drive microbe removal. By binding to C3a receptor (C3aR), C3a exhibits potent anaphylatoxin activity, including increased vascular permeability, triggering degranulation of mast cells, inflammation, and activating leukocytes. |
| Molecular Mass | 9.2 KDa |
| Apmol Mass | 9 KDa, reducing conditions |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Not tested |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Usage | For Research Use Only |
Target Details
| Target Function | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. |
| Subcellular Location | Secreted. |
| Database References |
Gene Functions References
- It has been shown that key proteins in the lectin arm of this pathway, MASP1, MASP2 and C3, are expressed in the developing cortex and that neuronal migration is impaired in knockout and knockdown mice. PMID: 28462915
- Complement C3 role in the lung cancer progression. PMID: 29118090
- Data report C3 as a novel myogenic factor secreted by undifferentiated preadipocyte that enhances myogenic differentiation of fetal progenitor cells and adult cells. The results show that complement C3 molecule internalizes myogenic and adipogenic precursor cells and then promotes their differentiation. PMID: 28279023
- C3 deficiency can prolong MHC-II molecule mismatched skin allograft survival. PMID: 27641978
- C3 role in the retinal epithelium and photoreceptor degeneration PMID: 28348233
- These findings shed light on mechanisms of age-related retinal alterations by identifying C3 as a potential therapeutic target for retinal aging. PMID: 28928904
- These findings suggest that C3 protects from early glaucomatous damage, a process that may involve EGFR signaling and other immune responses in the optic nerve head. PMID: 28446616
- complement C3 or downstream complement activation fragments may play an important role in Abeta plaque pathology. PMID: 28566429
- C5 and C5aR have critical roles in the development of C3 glomerulopathy. PMID: 28139294
- Data show that months after irradiation (IR) complement component 3 (C3-/-) mice made fewer errors than WT mice in a reversal learning test indicating better learning capacity in C3-/- mice after IR. PMID: 27029069
- Study show s the regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina PMID: 27564415
- Time-lapse video microscopy established the localization of the complement anaphylatoxin C3a and its receptor on enteric neural crest cells during their migration in the embryonic gut. C3a plays a role in regulating collective and directional cell migration, and in ganglia network organization during enteric nervous system ontogenesis. It regulates cell migration in a N-cadherin-dependent process. PMID: 27041467
- nutrient sensing in the liver is coupled to release of C3 and potentially its metabolic and inflammatory functions. PMID: 27735945
- Retinal C3 was expressed by microglia/macrophages located in the outer retina in AMD eyes. In rodent photo-oxidative damage, C3-expressing microglia/macrophages and complement activation were located in regions of lesion expansion in the outer retina over 2 months PMID: 28605809
- Wild-type C57BL/6 mice with pristane-induced lupus developed a strong IFN signature, which was absent in immunoglobulin-deficient (muMT), C3(-/-) , and CD18(-/-) mice. In vivo phagocytosis of dead cells was impaired in C3-deficient mice. PMID: 27274010
- Study found that cancer-cell-derived C3 activates the C3a receptor in the choroid plexus epithelium to disrupt the blood-cerebrospinal fluid (CSF) barrier. This effect allows plasma components, including amphiregulin, and other mitogens to enter the CSF and promote cancer cell growth. PMID: 28283064
- We induced anti-myeloperoxidase vasculitis in mice and confirmed a role for complement activation by demonstrating protection in C3-deficient mice. PMID: 27235854
- These data indicated that alpha7-nAChR caused the inhibition of ASPinduced activation of p38 kinase and NFkappa B to inhibit the production of MCP1 and keratinocytederived chemokine. PMID: 27572255
- These results suggest that GAS utilizes diverse mechanisms to degrade C3b and thus to protect bacterial cells from the complement response of the host. PMID: 26945067
- This study provideed the evidence that C3a plays a critical role in cerebral endothelial activation and leukocyte recruitment during inflammation in the brain. PMID: 26822321
- Demonstrate that cardiac Sirt1 plays an essential role in caloritc restriction-induced cardioprotection against myocardial I/R injury by suppressing cardiac C3 expression. PMID: 26873964
- The roles of C3, CfB, and C3a receptor in the severity of S. aureus induced septic arthritis are reported. PMID: 26787717
- Study shows that C3a is locally produced by -retinal pigment epithelial cells suggesting a key role in early macular degenerations pathogenesis. PMID: 26199322
- Complement C3 activation occurs following intramuscular islet transplantation, but it does not seem to affect the graft function. PMID: 26900812
- Studied whether increased levels of complement in HD brains contributed to disease progression in the R6/2 mouse model of HD. Found C3 deficiency does not alter HD progression in the R6/2 mouse model. PMID: 23097680
- Identify complement 5a as the major C3 activation product that was involved in macrophage polarization and DOCA-salt-induced vascular injury. PMID: 25573852
- our findings define the mechanism of receptor crosstalk between CR3 and Dectin-1 and demonstrate the importance of their collaboration in host defense against fungal infection. PMID: 26132276
- Carboxypeptidase B2 deficiency reveals that complement C3a limits infection in a murine polymicrobial sepsis model. PMID: 25851247
- Complement component 3 is up-regulated via non-canonical TGF-beta signaling in the retinal pigment epithelium PMID: 25802332
- Data indicate that for all three pathways there was a strong correlation between the amount of Ccomplement C3 (C3) fragments and the reduction in functional complement activity. PMID: 25733354
- Therapeutic efficacy of radiotherapy depends on C3a and C5a. PMID: 25888260
- C3 secreted from mycoplasma-infected Mesenchymal stem cells (MSCs) has an important role in the immunomodulatory functions of MSCs. PMID: 24763049
- Data indicate that absence of complement C3, complement C5 and Fc receptor subunit gamma (FcRgamma) does not impact autoantibody production in K/BxN mice. PMID: 23237573
- These results suggest that osteoclast-derived C3a functions in the relay from bone resorption to formation and may be a candidate for a coupling factor. PMID: 24470120
- Coagulation cascades, by generating thrombin and plasmin, respectively, provide C5 convertase activity, explaining why mobilization of hematopoietic stem cells in C3-deficient mice. PMID: 24667943
- These results provide new insights into the role of systemic C3 in regulating contraction following intracellular bacterial infection and may help to develop vaccines that are more effective. PMID: 25187659
- a role for outside-in iC3b-CD11b signals in limiting intrinsic organ inflammation PMID: 24632830
- have identified Complement C3 as a candidate plasma biomarker for measuring disease state in neuroblastoma PMID: 24200836
- A previously unknown function of C3a promotes protection to both myeloid and lymphoid cells against Listeria monocytogenes-induced apoptosis. PMID: 24981453
- Genetic and intervention studies implicating complement C3 as a major target for the treatment of periodontitis. PMID: 24808362
- Loss of dopaminergic neurons was rescued by complement C3-deficient mice. PMID: 24948809
- Complement protein C3 exacerbates prion disease in a mouse model of chronic wasting disease. PMID: 24038599
- Complement C3 and C5 deficiency affects bone fracture healing. PMID: 24260573
- Radiation combined injury-induced alterations of corticosterone, CRP, C3, IgM, and PGE2 cause homeostatic imbalance and may contribute to reduced survival. PMID: 24175013
- Data indicate that co-deficiency of factor H (FH) and MASP-1/MASP-3 did not ameliorate either the plasma Complement C3 (C3) activation or glomerular C3 accumulation in FH-deficient mice. PMID: 24279761
- The results of this study suggested that C3 plays a role in the regulation of the number and function of glutamatergic synapses in the hippocampus and exerts negative effects on hippocampus-dependent cognitive performance. PMID: 24378428
- C3 induces epithelial-to-mesenchymal transition and may be a primary factor to activate the renal renin-angiotensin systems to induce hypertension. PMID: 23926185
- Data indicate complement C3 deposition on peanut extract (PE) was abolished in immunoglobulin- and C4-deficient sera. PMID: 23182714
- ALI in H5N1-infected mice was caused by excessive complement activation, as demonstrated by deposition of C3, C5b-9, and MBL-C in lung tissue, and by up-regulation of MBL-associated serine protease-2 and the complement receptors C3aR and C5aR. PMID: 23526211
- in vitro results suggest various metabolic hormones and inflammatory factors can affect acylation stimulating protein (ASP) production and that ASP could constitute a new link between adipocytes and macrophage PMID: 23430572
