Recombinant Mouse CCL24 Protein

Name: Recombinant Mouse CCL24 Protein
Brand: Beta LifeScience
SKU: BL-1824NP
Availability: InStock

BL-1824NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Chemokines and receptors, Cytokines, Cytokines and growth factors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse C-C Motif Chemokine 24 is produced by our E.coli expression system and the target gene encoding Val27-Val119 is expressed.
Accession	Q9JKC0
Synonym	C-C motif chemokine 24;Ccl24;Eosinophil chemotactic protein 2;Eotaxin-2;Small-inducible cytokine A24;Scya24
Gene Background	Mouse CCL24 is a secreted protein, which is a member of the CC chemokine subfamily. Mouse Ccl24 cDNA encodes a 119 amino acid residue precursor protein, shares approximately 58% amino acid sequence identity with human Ccl24. It is predominantly expressed in the jejunum and spleen and also be induced in the lung by allergen challengeand IL4. Mouse ccl24 has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Ccl24 is chemotactic for resting T-lymphocytes, eosinophils and can bind to CCR3. LPS and IL4 also differentially regulate the expression of Ccl24 in monocytes and macrophages.
Molecular Mass	10.3 KDa
Apmol Mass	12 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: mmu:56221 STRING: 10090.ENSMUSP00000004936 UniGene: Mm.31505
Tissue Specificity	Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.

Gene Functions References

the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.