Recombinant Mouse CD40 Protein (His & Fc Tag)

Name: Recombinant Mouse CD40 Protein (His & Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-0993
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Car-nk targets proteins, Cluster of differentiation (cd) proteins, Co-stimulatory receptors, Featured cd protein molecules, Featured immune checkpoint protein molecules, Immune checkpoint proteins, Recombinant proteins

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Product Overview

Tag	His&Fc
Host Species	Mouse
Accession	P27512-1
Synonym	CD40 Molecule, TNF Receptor Superfamily Member 5, TNFRSF5,Tumor Necrosis Factor Receptor Superfamily, Member 5, Bp50, B-Cell SurfaceAntigen CD40, CD40L Receptor, CDW40, B Cell Surface Antigen CD40, BCell-Associated Molecule, CD40 Antigen (TNF Receptor Superfamily Member 5), CD40Type II Isoform, Nerve Growth Factor Receptor-Related B-Lymphocyte ActivationMolecule, p50, Tumor Necrosis Factor Receptor Superfamily Member 5, CDw40, CD40 Antigen.
Background	CD40 belongs to the TNF-receptor super family. CD40 has been found to be vital in mediatinga wide range of immune and inflammatory responses including T cell-dependentimmunoglobulin class switching,memory B cell development, and germinal centerformation. AT-hook transcription factor AKNA is accounted to coordinately regulate theexpression of CD40 and its ligand, which is significant for homotypic cell interactions. Adaptorprotein TNFR2 interacts with CD40 and functions as a mediator of the signal transduction.The interaction of CD40 and its ligand is found to be essential foramyloid-beta-induced microglialactivation, and therefore is considered to be an early event in Alzheimerdisease pathogenesis.
Description	A DNA sequence encoding the extracellular domain of mouse CD40 isoform 1 (P27512-1) (Met 1-Arg 193) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Leu 20
AA Sequence	Met 1-Arg 193
Molecular Weight	The secreted recombinant mouse CD40/Fc is a disulfide-linked homodimer The reduced monomer comprises 422 amino acids and has a predicted molecular mass of 47 kDa. As a result of glycosylation, it migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
Purity	Greater than 90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to bind recombinant human CD40L in a functional ELISA.
Formulation	Lyophilized from sterile PBS, pH 7.4
Stability	Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C
Usage	For Research Use Only
Storage	Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.