Recombinant Mouse CD80 Protein

Name: Recombinant Mouse CD80 Protein
Brand: Beta LifeScience
SKU: BL-0082PS
Availability: InStock

Collections: Cluster of differentiation (cd) proteins, Co-inhibitory receptors, Co-stimulatory receptors, Featured cd protein molecules, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	His
Host Species	Mouse
Synonym	LAB7,CD28LG, CD28LG1, CD28LG1, B71.
Background	The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells CD-80 is involved in the costimulatory signal essential for t- lymphocyte activation. t-cell proliferation and cytokine production is induced by the binding of cd28 or ctla-4 to this receptor.
Description	CD80 expressed in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 216a.a. (37-246 a.a.) andhaving a molecular weight of 24.7kDa (Migrates at 28-40kDa on SDS-PAGE underreducing conditions).CD80 is expressed with a 6a.a. His tag atC-Terminus and purified by unique purification methods.
Source	Sf9
AA Sequence	DVDEQLSKSV KDKVLLPCRY NSPHEDESED RIYWQKHDKV VLSVIAGKLKVWPEYKNRTL YDNTTYSLII LGLVLSDRGT YSCVVQKKER GTYEVKHLAL VKLSIKADFS TPNITESGNPSADTKRITCF ASGGFPKPRF SWLENGRELP GINTTISQDP ESELYTISSQ LDFNTTRNHT IKCLIKYGDAHVSEDFTWEK PPEDPPDSKN HHHHHH.
Purity	Greaterthan 95.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	CD80 protein solution (0.25mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.