Recombinant Mouse CDCP1 Protein (Fc Tag)

Name: Recombinant Mouse CDCP1 Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-1183
Availability: InStock

Collections: Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Mouse
Accession	Q5U462
Synonym	9030022E12Rik, AA409659, E030027H19Rik
Background	CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA42 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.
Description	A DNA sequence encoding the mouse CDCP1 (Q5U462) (Met1-Leu666) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Ser 30
AA Sequence	Met1-Leu666
Molecular Weight	The recombinant mouse CDCP1 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 878 a.a. and has a predicted molecular mass of 98.8 kDa. The apparent molecular mass of the protein is approximately 117 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>79% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Subcellular Location	Cell membrane; Single-pass membrane protein.
Database References	KEGG: mmu:109332 STRING: 10090.ENSMUSP00000042057 UniGene: Mm.27519

Gene Functions References

Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of autoimmune diseases. PMID: 28760953
Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells. PMID: 23243018
established mechanism for initiation of CDCP1-mediated signaling; demonstrated that processing of surface CDCP1 by extracellular serine proteases is prerequisite for CDCP1-dependent survival of cancer cells during metastasis; preventing cleavage of CDCP1 abrogated survival signaling associated with CDCP1 and induced PARP1 cleavage and PARP1-mediated apoptosis PMID: 22179830
CDCP1 may have a role in neoplasm metastasis; targeting it with a monoclonal antibody inhibits metastasis in a prostate cancer model PMID: 18483259

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.