Recombinant Mouse Complement C1Q Tumor Necrosis Factor-Related Protein 6 (C1QTNF6) Protein (His)

Name: Recombinant Mouse Complement C1Q Tumor Necrosis Factor-Related Protein 6 (C1QTNF6) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06634P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Recombinant proteins, Tumor necrosis factors and receptors (tnfs)

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Product Overview

Description	Recombinant Mouse Complement C1Q Tumor Necrosis Factor-Related Protein 6 (C1QTNF6) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q6IR41
Target Symbol	C1QTNF6
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	C-6His
Target Protein Sequence	VPTEETTFGESVASHLPKGCRRCCDPEDLMSSDDTVQAPVSPYVLPEVRPYINITILKGDKGDRGPTGTPGKPGKNGTRGDRGSQGVKGDKGQAGSPGSSCQTHYSAFSVGRKTGLHSSENFLSLLFDRVFVNTDGHFDMATGSFVAPLRGLYFFSLNVHSWNYKETYVHIVHNEQAVVILYAQPSERSIMQSQSVMLPLVPGDRVWVRLFKRERENGIYSDDVDTYITFSGHLIKAEDN
Expression Range	25-264aa
Protein Length	Full Length
Mol. Weight	27.9 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Subcellular Location	Secreted.
Database References	KEGG: mmu:72709 STRING: 10090.ENSMUSP00000023075 UniGene: Mm.34776

Gene Functions References

The results establish CTRP6 as a novel metabolic/immune regulator linking obesity to adipose tissue inflammation and insulin resistance. PMID: 28726640
CTRP6 knockdown down-regulated the levels of lipogenic genes and phosphorylated Erk1/2 (p-Erk1/2) in the early lipogenic stage, whereas up-regulated p-Erk1/2 in the terminal differentiation in skeletal myoblasts. PMID: 27125977
CTRP6 specifically suppresses the alternative pathway of the complement system. C1qtnf6(-/-) mice are highly susceptible to induced arthritis, Arthus reaction, and experimental autoimmune encephalomyelitis. PMID: 26404464
Knockdown of CTRP6 can inhibit adipogenesis of 3T3-L1 adipocytes through lipogenic marker genes and Erk1/2 signaling pathway. PMID: 25639984
CTRP6 plays a role in fatty acid metabolism via the AMPK-ACC pathway. The globular domain of CTRP6 mediates the phosphorylation and activation of the 5'-AMP-activated protein kinase (AMPK) in skeletal muscle cells. PMID: 20102710

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.