Recombinant Mouse Eotaxin Protein (CCL11), Active

Name: Recombinant Mouse Eotaxin Protein (CCL11), Active
Brand: Beta LifeScience
SKU: BLC-05474P
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Chemokines and receptors, Cytokines, Cytokines and growth factors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Eotaxin Protein (CCL11), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 96% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
Uniprotkb	P48298
Target Symbol	CCL11
Synonyms	Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Expression Range	24-97aa
Protein Length	Full Length of Mature Protein
Mol. Weight	8.4 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: mmu:20292 STRING: 10090.ENSMUSP00000000342 UniGene: Mm.4686
Tissue Specificity	Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Gene Functions References

the adipose-derived FGF21-CCL11 axis triggers cold-induced beiging and thermogenesis by coupling sympathetic nervous system to activation of type 2 immunity in subcutaneous white adipose tissue. PMID: 28844880
CCL11 promotes migration and proliferation of mouse neural progenitor cells. PMID: 28173860
These studies characterized serum and intestinal wall eotaxin-1 levels in various inflammatory bowel disease patients and to explore the effect of targeting eotaxin-1 by specific antibodies in dextran sodium sulfate-induced colitis model. PMID: 26874691
this study shows that eosinophil trafficking to the heart is dependent on the eotaxin-CCR3 pathway in a mouse model of experimental autoimmune myocarditis PMID: 27621211
Blocking antibodies against RANTES and eotaxin reduced the infiltration of CD4(+) and CD8(+) T cells into the nigra, attenuated nigral expression of proinflammatory molecules, and suppressed nigral activation of glial cells. These findings paralleled dopaminergic neuronal protection, normalized striatal neurotransmitters, and improved motor functions in MPTP-intoxicated mice. PMID: 27226559
These results indicate that CCL11 was responsible for the limited angiogenesis and necrosis by inducing and attracting eosinophils in the tumors. PMID: 27169545
Study demonstrated that CCL11 is primarily produced by activated astrocytes in the CNS, activates microglia to produce ROS via NOX1, and exacerbates excitotoxic neuronal death PMID: 26184677
PAR2 activation through endogenous mast cell tryptase activity could be required, at least partially, to mediate CCL11-induced eosinophil migration PMID: 24972241
The chemokines monocyte chemotactic protein 1 (MCP1), MIP1alpha, MIP1beta, interferon gamma-induced protein 10 (IP-10), and eotaxin were induced in Saa1 TG mice. PMID: 25847238
investigated role of Eotaxin-1 on disease outcome in Litomosoides sigmodontis infection; findings suggest, in Eotaxin-1(-/-) mice, potential reduced activation state of eosinophils; macrophages produce decreased amounts of IL-6 in vitro suggesting possible mechanisms by which Eotaxin-1 regulates activation of inflammatory cells and parasite survival PMID: 24112106
Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
TNC expression controls eotaxin level in apo E-/- mice and that this chemokine plays a key role in the development of atherosclerosis PMID: 23433402
Data indicate that the combination of Ovalbumin (OVA) and hypoxia induced a enhanced expression of HIF-1alpha and increased eotaxin-1, lung TGB-beta1 expression, and indices of airway remodeling. PMID: 23499929
increased sputum and nasal lavage fluid levels in allergic rhinitis subjects PMID: 21703102
These studies demonstrate that inflammatory monocyte/macrophage-derived CCL11 drives colonic eosinophilic inflammation in experimental colitis. PMID: 21498668
The oesophageal production of CCL11 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
Data show that that augmented airway eosinophilic inflammation and hyperresponsiveness in RV-infected mice with allergic airways disease is directed in part by eotaxin-1. PMID: 20644177
eotaxin initiates allergic airway disease due to A. fumigatus, but this chemokine did not appear to contribute to the maintenance of A. fumigatus-induced allergic airway disease. PMID: 12060577
concentrations of eotaxin in the CSF of Angiostrongylus cantonensis infected mice each week after infection were all significantly higher than those in serum ( P<0.0001). PMID: 14648203
CCL11 is a potent chemotactic factor for smooth muscle cells. Because CCL11 is expressed abundantly in SMC-rich areas of the atherosclerotic plaque and in injured arteries, it may play an important role in regulating SMC migration. PMID: 15130922
infection of mice by Histoplasma capsulatum induced rapid generation of high levels of MIP-1alpha, which remained elevated from 4-48 h whereas very little eotaxin was detected at any time point PMID: 15316665
critical role for eotaxin-1 in Brugia malayi microfilaria parasite clearance PMID: 15593125
Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
The eotaxin-1 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
CCL11 and CCR3 are important in the pulmonary recruitment of granulocytes and play significant pathogenic roles in bleomycin-induced lung fibrosis. PMID: 16314464
IL-4 induction and the IL-4/OSM synergistic induction of eotaxin-1 was abrogated in STAT6(-/-) mouse lung fibroblasts, however, regulation of IL-6 was similar in -/- or wild-type mouse lung fibroblasts PMID: 16547273
CCL11 is the salient but not the sole eosinophil chemoattractant of biological significance during gastrointestinal helminth infection. PMID: 16783848
eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
In the absence of eotaxin-2 or CCR3, there was a profound reduction in IL-13-induced eosinophil recruitment into the lung lumen. In the absence of eotaxin-1, there was a fourfold increase in IL-13-mediated eosinophil recruitment into the airway. PMID: 17148674
There was a higher tumor incidence in CCL11(-/-) BALB/c mice, which was associated with a reduced eosinophil influx into tumors. PMID: 17371978
Heligmosomoides infection led to reduction in number of lung eosinophils with decreased levels of eotaxin in bronchoalveolar lavage fluid, lowered of CCR3 receptor expression on eosinophils and impaired chemotaxis of these cells toward eotaxin. PMID: 17650182
induction of eotaxin-1 and CD4+ T cell production of IL-5 are required for respiratory syncytial virus G glycoprotein-induced eosinophilia following respiratory syncytial virus challenge. PMID: 18519743
Specific inhibition of CCL11 alone is therefore unlikely to inhibit eosinophil recruitment to the airways. PMID: 18850374
Data show thatprimary or secondary resistance were unaffected at either the pre-lung or gut stages of infection in eotaxin(-/-) single mutant mice. PMID: 19535141

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.