Recombinant Mouse Factor X Protein (C-6His)

Name: Recombinant Mouse Factor X Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0940NP
Availability: InStock

BL-0940NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Recombinant proteins

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Product Overview

Description	Recombinant Mouse CoagulationFactor X is produced by our Mammalian expression system and the target gene encoding Gly21-Asn481 is expressed with a 6His tag at the C-terminus.
Accession	O88947
Synonym	F10;Coagulation factor X;Stuart factor
Gene Background	Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.
Molecular Mass	34.6&18.4 KDa
Apmol Mass	50-60&20-28 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 1mM CaCl2, pH 7.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Subcellular Location	Secreted.
Protein Families	Peptidase S1 family
Database References	KEGG: mmu:14058 STRING: 10090.ENSMUSP00000033821 UniGene: Mm.262589
Tissue Specificity	Plasma; synthesized in the liver.

Gene Functions References

PTX2 was identified PTX2 as a novel partner for FX, and both proteins cooperated to prevent their SR-AI-mediated uptake by macrophages. PMID: 28213380
Enhanced FXa and PAR2 exacerbate DN and that both are promising targets for preventing diabetic nephropathy. PMID: 27283743
Macrophages regulate FX plasma levels in an SR-AI-dependent manner. PMID: 26608330
Factor Xa has a role in inhibiting HMGB1-induced septic responses in human umbilical vein endothelial cells and in mice PMID: 25007770
Selective inhibition of FXa improves the left ventricular function during CVB3-induced myocarditis and seems to be associated with an improved myocardial remodeling. PMID: 24533719
Activated factor X signaling via protease-activated receptor 2 suppresses pro-inflammatory cytokine production from lipopolysaccharide-stimulated myeloid cells. PMID: 23872307
Differential effects of murine and human factor X on adenovirus transduction via cell-surface heparan sulfate. PMID: 21596747
There was no detectable increase in plasma levels of mouse FX after active-site inhibited human APC administration to mice overexpressing human EPCR. FX does not effectively interact with EPCR in vivo, at least in regards to the mouse system. PMID: 21885613
investigation of role of F10a in progression of diabetic nephropathy: data from studies using inhibitor of F10a suggest that F10a does play a role in development of proteinemia, glomerular hypertrophy, and protein deposition in kidney of db/db mice PMID: 21628879
Data suggest that tissue factor and factor V induction by LPS may in part accelerate mesangioproliferative glomerulonephritis through activation of factor X and downstream proinflammatory and procoagulant mechanisms. PMID: 19546589
Gene targeting of tissue factor, factor X, and factor VII in mice: their involvement in embryonic development PMID: 11841337
Factor Xa functions in airway remodeling in asthma by stimulating mucin production, through regulation of amphiregulin expression and collagen deposition. PMID: 17082493
Complete absence of FX is incompatible with murine survival. Minimal FX activity as low as 1-3% is sufficient to rescue the lethal phenotype. PMID: 18036190
Results show that although factor Xa induces p42/44 MAP Kinase phosphorylation in endothelial cells, it has no direct effect on endothelial cell proliferation, protein synthesis and tube formation. PMID: 19339215
expression of coagulation factor X (FX) is locally increased in fibrotic lung tissue, with marked immunostaining associated with bronchial and alveolar epithelia PMID: 19652365

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.