Recombinant Mouse FGF18 Protein (His Tag)

Name: Recombinant Mouse FGF18 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2040
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	NP_032031.1
Synonym	FGF18, FGF-18, ZFGF5, fibroblast growth factor 18
Background	Fibroblast growth factor 18 is a protein that in humans is encoded by the FGF18 gene. FGF18 (Fibroblast Growth Factor 18) is a Protein Coding gene. Diseases associated with FGF18 include Dyslexia. Among its related pathways are Signaling by activated point mutants of FGFR3 and PI3K/AKT activation. Gene Ontology (GO) annotations related to this gene include growth factor activity and type 2 fibroblast growth factor receptor binding. An important paralog of this gene is FGF8.
Description	A DNA sequence encoding the mouse FGF18 (Met 1-Gly 207) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Glu 28
AA Sequence	Met 1-Gly 207
Molecular Weight	The secreted recombinant mouse FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.5 kDa. As a result of glycosylation, it migrates as an approximately 30-35 kDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse FGF18 at 10 ug/mL (100 ul/well) can bind- Rat FGFR4, The EC50 of Rat FGFR4 is 0.44 ug/mL.
Formulation	Recombinant Mouse FGF18 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Mouse FGF18 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.