Recombinant Mouse FGF2 Protein (His Tag)

Name: Recombinant Mouse FGF2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2043
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant fibroblast growth factor basic (fgfb/fgf2/bfgf) proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	NP_032032.1
Synonym	FGF2, BFGF, FGF-2, FGFB, HBGF-2, fibroblast growth factor 2
Background	FGF2, also known as basic fibroblast growth factor (bFGF) and FGF-beta, is a growth factor and signaling protein encoded by the FGF2 gene.It is synthesized primarily as a 155 amino acid polypeptide, resulting in an 18 kDa protein. However, there are four alternate start codons which provide N-terminal extensions of 41, 46, 55, or 133 amino acids, resulting in proteins of 22 kDa (196 aa total), 22.5 kDa (201 aa total), 24 kDa (210 aa total) and 34 kDa (288 aa total), respectively.Generally, the 155 aa/18 kDa low molecular weight (LMW) form is considered cytoplasmic and can be secreted from the cell, whereas the high molecular weight (HMW) forms are directed to the cell's nucleus.
Description	A DNA sequence encoding the mouse Fgf2 (Ala11-Ser154) was expressed with a His tag at the N-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Ala11-Ser154
Molecular Weight	The recombinant mouse Fgf2 consists 162 amino acids and predicts a molecular mass of 18.5 kDa.
Purity	>95% as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Bioactivity	Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.1-0.6 ng/mL.
Formulation	Recombinant Mouse FGF2 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4.
Stability	Recombinant Mouse FGF2 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.