Recombinant Mouse FGFR1 Protein (Fc Tag)

Name: Recombinant Mouse FGFR1 Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-2056
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Mouse
Accession	P16092
Synonym	FGFR1, BFGFR, CD331, CEK, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1, ECCL, fibroblast growth factor receptor 1
Background	Fibroblast growth factor receptor 1 (FGFR1), also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2 / Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. FGFR1 has been shown to be associated with Pfeiffer syndrome.FGFR1 is a member of the fibroblast growth factor receptor (FGFR) family, which in addition to FGFR1, includes FGFR2, FGFR3, FGFR4, and FGFRL1. FGFR1-4 are cell surface membrane receptors that possess tyrosine kinase activity.
Description	A DNA sequence encoding the extracellular domain (Met 1-Glu 376) of mouse FGFR1 isoform 1 was as fused with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Arg 22
AA Sequence	Met 1-Glu 376
Molecular Weight	The secreted recombinant mouse FGFR1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 596 amino acids and has a calculated molecular mass of 66.5 kDa. The apparent molecular mass of rmFGFR1/Fc monomer is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1).
Formulation	Recombinant Mouse FGFR1 Protein (Fc Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Mouse FGFR1 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.