Recombinant Mouse Fibroleukin (FGL2) Protein (His)

Name: Recombinant Mouse Fibroleukin (FGL2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01645P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Fibroleukin (FGL2) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P12804
Target Symbol	FGL2
Synonyms	Cytotoxic T-lymphocyte-specific protein;Fibrinogen-like protein 2;Prothrombinase
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	VEEHNLTEGLEDASAQAACPARLEGSGRCEGSQCPFQLTLPTLTIQLPRQLGSMEEVLKEVRTLKEAVDSLKKSCQDCKLQADDHRDPGGNGGNGAETAEDSRVQELESQVNKLSSELKNAKDQIQGLQGRLETLHLVNMNNIENYVDNKVANLTVVVNSLDGKCSKCPSQEHMQSQPVQHLIYKDCSDHYVLGRRSSGAYRVTPDHRNSSFEVYCDMETMGGGWTVLQARLDGSTNFTREWKDYKAGFGNLEREFWLGNDKIHLLTKSKEMILRIDLEDFNGLTLYALYDQFYVANEFLKYRLHIGNYNGTAGDALRFSRHYNHDLRFFTTPDRDNDRYPSGNCGLYYSSGWWFDSCLSANLNGKYYHQKYKGVRNGIFWGTWPGINQAQPGGYKSSFKQAKMMIRPKNFKP
Expression Range	20-432aa
Protein Length	Full Length of Mature Protein
Mol. Weight	50.0 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Converts prothrombin to thrombin.
Subcellular Location	Secreted.
Database References	KEGG: mmu:14190 STRING: 10090.ENSMUSP00000046131 UniGene: Mm.292100
Tissue Specificity	Constitutively expressed in cytotoxic T-cells.

Gene Functions References

These results indicated that FGL2 might be a promising target for attenuating the severity of Severe acute pancreatitis (SAP) and adenovirus-mediated artificial miRNAs targetting FGL2 represented a potential therapeutic approach for the treatment of SAP. PMID: 29054965
The results of the present study indicated that, in the colitis model, FGL2 was associated with the immunopathogenesis of inflammatory bowel disease. PMID: 28713999
FGL2-overexpressing regulatory T-cells have enhanced immunosuppressive activity and prevent colitis; FGL2-over-expressing effector T-cells are hypo-proliferative and do not induce colitis PMID: 28487604
PD-1 plays a vital role in brain inflammation via regulation of Fgl-2 after ICH, and that manipulation of PD-1 might be a promising therapeutical target in ICH. PMID: 27717876
In vitro IL-33 treatment abrogated MHV-3 and IFN-gamma induced FGL2 expression in RAW264.7 and THP-1 cells. PMID: 28494352
FGL2 appears as one of the key players in immune regulatory processes favoring metacestode survival by promoting Regulatory T-cell activity and IL-17A production that contributes to FGL2-regulation. PMID: 25955764
these data strongly suggest that Treg cells expressing FGL2 mediate rapamycin-induced tolerance. Furthermore, a gene biomarker panel that includes fgl2 can distinguish between rejecting and tolerant grafts. PMID: 24990517
Mouse susceptibility to MHV-3-induced fulminant hepatitis may rely on C5a/C5aR interactions, for which ERK1/2 and p38 pathways participate in up-regulating Fgl2 expression. PMID: 24604562
Targeted deletion of FGL2 leads to increased early viral replication and enhanced adaptive immunity in a murine model of acute viral hepatitis. PMID: 24146739
Cysteines at positions 94, 97, 184 and 187, found in the coiled-coil domain were shown to be crucial for FGL2 oligomerization. PMID: 23127799
FGL2 binding to the FcgammaRIIB receptor expressed on sinusoidal endothelial cells (SECs) is a critical event in the initiation of the hepatic reperfusion injury cascade through induction of SEC and hepatocyte death. PMID: 21756857
The prothrombinase activity of FGL2 contributes to the pathogenesis of experimental arthritis. PMID: 21469939
Fgl2 cleaves prothrombin to thrombin consistent with serine protease activity and requires calcium, phospholipids, and factor Va for its full activity. PMID: 11994472
While membrane-bound Fgl2 acts as a prothrombinase, soluble Fgl2 is an immunomodulatory protein that has the ability to modulate T cell responses and to alter dendritic cell (DC) maturation to favor production of tolerogenic DC. PMID: 12682232
fgl2 expression in endothelial cells is controlled by Ets-1 and Oct-1, and requires Sp1 and Sp3 PMID: 12752447
Fgl2 is required for lipopolysaccharide-triggered abortions and for normal mouse reproduction. PMID: 14742694
Fgl2-deficient and control mice produce similar levels of fibrin, a product of the coagulation cascade, during T. gondii infection and allograft rejection. PMID: 14976252
Fgl2 is constitutively absent at baseline in vascular endothelial cells (ECs) of fgl2+/- murine hearts, but is induced in vascular ECs of mouse-to-rat cardiac xenografts undergoing acute vascular rejection with thrombosis. PMID: 15100314
Fgl2 expression accounts for the fibrin deposition seen in experimental cardiac allograft rejection; these data provide a rationale for targeting fgl2 as adjunctive therapy to treat allograft rejection. PMID: 15905589
Results suggest that virus-induced fibrinogen-like protein 2 prothrombinase/fibroleukin expression and associated coagulation activity play a pivotal role in initiating severe hepatitis. PMID: 16437596
FGL2 has a role in susceptibility to mouse hepatitis virus (MHV-3)-induced fulminant hepatitis PMID: 17037572
Fgl2 deficiency is not associated with obvious structural cardiac defects but is associated with a high incidence of neonatal death as well as contractile dysfunction and rhythm abnormalities during embryonic and postnatal development in mice PMID: 17550996
FGL2 contributes to Treg cell activity and inhibits the development of autoimmune disease. PMID: 18097026
FGL2 is an important effector cytokine of egulatory T cells that contributes to susceptibility to mouse hepatitis virus strain 3 induced fulminant viral hepatitis. PMID: 19085958

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.