Recombinant Mouse H-2 Class Ii Histocompatibility Antigen Gamma Chain (CD74) Protein (His)

Name: Recombinant Mouse H-2 Class Ii Histocompatibility Antigen Gamma Chain (CD74) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03105P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Cd74.

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Car-nk targets proteins, Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Description	Recombinant Mouse H-2 Class Ii Histocompatibility Antigen Gamma Chain (CD74) Protein (His) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P04441
Target Symbol	CD74
Synonyms	Cd74; Ii; H-2 class II histocompatibility antigen gamma chain; Ia antigen-associated invariant chain; Ii; MHC class II-associated invariant chain; CD antigen CD74) [Cleaved into: Class-II-associated invariant chain peptide; CLIP)]
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	QQQGRLDKLTITSQNLQLESLRMKLPKSAKPVSQMRMATPLLMRPMSMDNMLLGPVKNVTKYGNMTQDHVMHLLTRSGPLEYPQLKGTFPENLKHLKNSMDGVNWKIFESWMKQWLLFEMSKNSLEEKKPTEAPPKVLTKCQEEVSHIPAVYPGAFRPKCDENGNYLPLQCHGSTGYCWCVFPNGTEVPHTKSRGRHNCSEPLDMEDLSSGLGVTRQELGQVTL
Expression Range	56-279aa
Protein Length	Extracellular Domain
Mol. Weight	29.4kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.; Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).; Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
Subcellular Location	[Isoform Long]: Late endosome. Lysosome.; Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome.
Database References	KEGG: mmu:16149 STRING: 10090.ENSMUSP00000095171 UniGene: Mm.439737
Tissue Specificity	[Isoform Long]: Expressed in thymus and lymph noodes. Expressed by antigen-presenting cells (APCs).; [Isoform Short]: Expressed in thymus and lymph noodes.

Gene Functions References

House dust mites sensitization lowered humoral immune responses to intact pneumococcus and this effect was significantly modified by the HLA-DR polymorphism. PMID: 27506953
Data highlight the complexity of the MIF/CD74 signaling pathway in the development of mesothelioma. PMID: 26883190
We demonstrate that MHCII is dispensable for the B cell phenotype of (signal peptide peptidase-like 2a) SPPL2a(-/-) mice, further supporting a CD74-intrinsic effect. PMID: 28550201
CLIP expression and activated gamma-delta T cells are responsible for the development of preeclampsia. PMID: 28642294
MIF-CD74 signaling inhibits interferon (IFN)-gamma secretion in microglia through phosphorylation of microglial ERK1/2 (extracellular signal-regulated protein kinases 1 and 2). The inhibition of MIF signaling or its receptor CD74 promotes IFN-gamma release and amplifies tumor death either through pharmacological inhibition or through siRNA-mediated knockdown. PMID: 27157615
Ii mediated pathways play a vital role in the initiation and propagation of liver inflammation. PMID: 27371158
CD74-knockout mice showed disturbed placental morphology, reduced junctional zone, smaller placentas, and impaired spiral artery remodeling with fetal growth restriction. PMID: 27199465
explored CD74 expression, which can form a complex with CD44, in glomerular parietal epithelial cells during the progression of a model of focal segmental glomerulosclerosis PMID: 27463800
might play roles in both the middle ear and inner ear in lipopolysaccharide-induced otitis media PMID: 27181906
Data show that cathepsins S (CatS) regulates CCL2 chemokine expression by modulation of CD74 antigen processing. PMID: 26358505
Suggest that the DQA1*0103/CD74 dimer may result in presentation of unique antigens and susceptibility to develop arthritis. PMID: 26524976
CD74 plays a critical role in the MIF inhibition of osteoclastogenesis. PMID: 23044992
Genetic deletion of CD74 restored the ability of infected dendritic cells to present a parasite antigen in the context of MHC-II. PMID: 26195549
SPPL2a-mediated processing of CD74 NTF is indispensable to maintain appropriate levels of tonic BCR signaling to promote B cell maturation. PMID: 26157172
Results support the hypothesis that neurodegeneration following traumatic brain injury is dependent upon antigen processing and presentation that requires CD74 PMID: 25329434
Methylglyoxal induced gene expression of CD74 in the in the diabetic retina. PMID: 24974304
In TPA-induced skin inflammation, MIF is released from damaged keratinocytes and then triggers the chemotaxis of CD74(+)CXCR2(+) NKT cells for IFN-gamma production. PMID: 25172501
MIF promotes the migration of B cells through a ZAP-70-dependent pathway mediated by cooperative engagement of CXCR4 and CD74. PMID: 24760155
These results demonstrate natural antagonist activity of DRalpha1 for macrophage migration inhibitory factor PMID: 24683185
Recombinant vaccinia virus vaccines encoding CD74 may be useful tools to improve CD4 T-cell responses to viral and tumour antigens. PMID: 24205828
Macrophage migration inhibitory factor receptor CD74 mediates alphavirus-induced arthritis and myositis in murine models of alphavirus infection. PMID: 23896945
These findings implicate binding of recombinant T-cell receptor ligand constructs to CD74 as a key step in both antigen-driven and bystander T-cell tolerance important in treatment of inflammatory diseases. PMID: 23026773
Data show that human Iip35 isoform (CD74 antigen) is expressed in mouse antigen presenting cells. PMID: 22689013
regulation of CD74 levels by SPPL2a is indispensable for B cell development PMID: 23267015
). Proteolytic processing of CD74 requires SPPL2A. PMID: 23267016
These results emphasize the importance of Ii in B cell homeostasis and suggest that Iip35 could have regulatory functions. PMID: 22966065
demonstrate here a CD74-dependent MHC class I cross-presentation pathway in dendritic cells that had a major role in the generation of MHC class I-restricted, cytolytic T lymphocyte responses to viral protein- and cell-associated antigens PMID: 22306692
The cytokine midkine and its receptor RPTPzeta regulate B cell survival in a pathway induced by CD74. PMID: 22140262
MIF promoted the phosphorylation of AMP-activated protein kinase (AMPK) in a CD74-dependent manner and, in turn, inhibition of AMPK reversed the inhibition of PDGF-induced hepatic stellate cell activation by MIF. PMID: 21969590
iNKT developed in Ii(-/-) but not catS(-/-) mice have defective effector function. PMID: 21565221
Data demonstrate that MIF and CD74 play previously unappreciated roles in CCL2-induced macrophage adhesion and migration. PMID: 21411731
this study emphasizes the role of microvesicles and Ii in the communication between DC and microglia. PMID: 20816669
although other regions of Ii interact with class II, CLIP binding to the groove is likely to be a dominant event in assembly of nascent class II molecules with Ii in the ER PMID: 20547545
expression and function of CD74 in normal colon epithelial cells and colon carcinoma cells PMID: 20614481
Data show that D74-HGF/c-Met axis defines a novel physiologic survival pathway in mature B cells, resulting in the control of the humoral immune response. PMID: 20639480
We here identify Cd74 as a common insertion site in murine B-lymphomas PMID: 20416035
Preferential Th1 immune response in invariant chain knockout mice PMID: 11823488
structure for the trimeric MHC class II-associated invariant chain transmembrane domain PMID: 12126629
Expression and function of transgenic HLA-DQ molecules and lymphocyte development in mice lacking invariant chain. PMID: 12165499
major histocompatibility complex class II-associated invariant chain controls the activity of extracellular cathepsin L PMID: 12417635
Ii and its isoforms are essential to mediate ovalbumin-induced pulmonary inflammation, IgE generation, and airway hyperresponsiveness in a Th2-dependent immune response. PMID: 12538710
Expression of Ii blocks constitutive major histocompatibility (MHC) class II localization to plasma membrane lipid rafts and may reduce vaccine efficiency of M12.C3 B cell lymphoma and SaI-Ak sarcoma tumor vaccine cells. PMID: 14707062
Ii deficiency induces cell autonomous defects of two distinct B cell lineages: the life span of mature follicular B cells is reduced, accounting for their markedly decreased frequency, whereas, in contrast, marginal zone B cells accumulate. PMID: 14764672
In p41 CD74 isoform-transgenic mice on an I-A(d) background (p41-I-A(d) mice), the recovery from CNS injuries was worse than that of controls. PMID: 17182551
Ii siRNA-transfected H-2(K) DCs enhanced Th1 responses. PMID: 17296316
These results suggest that invariant chain-chondroitin sulfate may play an important role in the generation of cell-surface pools of invariant chain that can serve as receptors for CD44 and macrophage migration inhibitory factor. PMID: 17492940
Ii-Key modified HER-2/neu776-790 hybrid peptides are sufficiently potent to provide antigen-specific CD4+ TH cells with therapeutic antitumor activity PMID: 17634957
the CD74/NF-kappaB/TAp63 axis defines a novel antiapoptotic pathway in mature B cells, resulting in the shaping of both the B-cell repertoire and the immune response PMID: 17846227
impact of allelic variation in CLIP affinity on immune responses will be highest in cells with limited DM activity PMID: 17947664
NOS2 interacts with CD74 (the MHC II-associated invariant chain), and the degradation of CD74 by caspases in immature dendritic cells was inhibited upon treatment with nitric oxide donor. PMID: 18003616

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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