Recombinant Mouse Hepatoma-Derived Growth Factor (HDGF) Protein (His&Myc)

Name: Recombinant Mouse Hepatoma-Derived Growth Factor (HDGF) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-10786P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Hdgf.

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Product Overview

Description	Recombinant Mouse Hepatoma-Derived Growth Factor (HDGF) Protein (His&Myc) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P51859
Target Symbol	HDGF
Synonyms	Hdgf; Tdrm1; Hepatoma-derived growth factor; HDGF
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	N-10His&C-Myc
Target Protein Sequence	MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL
Expression Range	1-237aa
Protein Length	Full Length
Mol. Weight	30.3kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein.
Subcellular Location	Nucleus. Cytoplasm. Secreted, extracellular exosome.
Protein Families	HDGF family
Database References	KEGG: mmu:15191 STRING: 10090.ENSMUSP00000005017 UniGene: Mm.292208
Tissue Specificity	Expressed predominantly in testis and skeletal muscle, to intermediate extents in heart, brain, lung, liver, and kidney, and to a minimal extent in spleen.

Gene Functions References

liver cancer cell-derived HDGF can induce Foxp3(+) T cells; the latter has immune suppressor functions on CD8(+) T cell activities PMID: 25569374
Up-regulation of hepatoma-derived growth factor facilities tumor progression in malignant melanoma. PMID: 23536873
HDGF promotes tumor progression after secondary upregulation and may represent another protein fitting into the concept of non-oncogene addiction of tumor tissue. PMID: 22014102
HDGF exploits the innate properties of both cell surface heparan sulfates and membrane receptor via the HATH domain to affect related cell signalling processes PMID: 20964630
HDGF plays a pro-fibrogenic role during liver fibrosis in mice through activation of TGF-beta pathway. PMID: 19913322
Hepatoma-derived growth factor stimulates cell growth after translocation to the nucleus PMID: 11751870
Hepatoma-derived growth factor helps regulate the hepatocyte proliferation in liver development. PMID: 12447878
Increased HDGF expression is correlated with disease progression of hepatocellular carcinoma and represent a specific prognostic factor in patient with liver cancer. PMID: 14508832
we investigated the role of HDGF in tumorigenesis and elucidated the mechanism of action. PMID: 14662017
HDGF is a novel type of neurotrophic factor harbored in the nucleus and it functions in an autocrine manner. PMID: 15140875
hepatoma-derived growth factor has roles in development and intestinal neoplasms PMID: 15604097
HDGF is a novel trophic factor for motor neurons and it might play a protective role against motor neuron degeneration. PMID: 16987236
The lack of obvious biochemical and morphological phenotypes in HDGF-deficient mice demonstrates that in vivo HDGF is dispensable for normal development in mice. PMID: 18570251

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.