Recombinant Mouse IGFBP-5 Protein (C-6His)
Beta LifeScience
SKU/CAT #: BL-0757NP
BL-0757NP: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Recombinant Mouse IGFBP-5 Protein (C-6His)
Beta LifeScience
SKU/CAT #: BL-0757NP
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Mouse Insulin-like Growth Factor-binding Protein 5 is produced by our Mammalian expression system and the target gene encoding Leu20-Glu271 is expressed with a 6His tag at the C-terminus. |
Accession | Q07079 |
Synonym | BP-5; IGFBP-5; IGF-binding protein 5; Insulin-like growth factor-binding protein 5; |
Gene Background | Mouse Insulin-like growth factor-binding protein 5(IGFBP-5) belongs to the superfamily of insulin-like growth factor (IGF) binding proteins. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. Mouse IGFBP-5 shows 97% aa sequence identity with those of human and rat IGFBP-5. It is expressed mostly in kidney, uterus and gastrocnemius muscle. It also expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
Molecular Mass | 29.3 KDa |
Apmol Mass | 38 KDa, reducing conditions |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Purity | Greater than 90% as determined by reducing SDS-PAGE. (QC verified) |
Biological Activity | Not tested |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping | The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
Usage | For Research Use Only |