Recombinant Mouse IL-1RA Protein

Name: Recombinant Mouse IL-1RA Protein
Brand: Beta LifeScience
SKU: BL-1910NP
Availability: InStock

BL-1910NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-1 Receptor Antagonist Protein is produced by our E.coli expression system and the target gene encoding Arg27-Gln178 is expressed.
Accession	P25085
Synonym	IL-1RN;IL-1ra;IRAP;IL1 inhibitor;Il1rn;Interleukin-1 Receptor Antagonist Protein
Gene Background	Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouse Il1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26% and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.
Molecular Mass	17.5 KDa
Apmol Mass	16 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Subcellular Location	[Isoform 1]: Secreted.; [Isoform 2]: Cytoplasm.
Protein Families	IL-1 family
Database References	KEGG: mmu:16181 STRING: 10090.ENSMUSP00000110126 UniGene: Mm.882

Gene Functions References

Findings identify a critical role for IL1Ra in maintaining the natural diversity and composition of intestinal microbiota, and suggest a role for TLR4 in mucosal Th17 cell induction associated with the development of autoimmune disease in mice. PMID: 28645307
The current study is the first to demonstrate ex vivo airway hyperreactivity caused by systemic maternal inflammation and postnatal hyperoxia, and it reveals further preclinical mechanistic insights into IL-1Ra as a treatment targeting key pathophysiological features of Bronchopulmonary dysplasia. PMID: 27482635
we demonstrate that the release of IL1RA in response to genotoxic stress contributes to the immunosuppressive effects of apoptotic cells. PMID: 26873573
results provide evidence of the key role of IL1RA secreted by MSCs to both control the polarization of macrophages toward a M2 phenotype and inhibit B cell differentiation in vivo. PMID: 26661518
Allo-transplantation of mesenchymal stem cells reduces Con A liver injury by increasing IL10 production through an IL1Ra dependent macrophage switch. PMID: 26276675
results demonstrate that small intestinal eosinophils play a pivotal role in the maintenance of intestinal homeostasis by regulating Th17 cells via production of IL-1Ra. PMID: 26951334
While both contributing to pathogen clearance, NLRP3 more than NLRC4 contributes to deleterious inflammatory responses in cystic fibrosis and correlates with defective NLRC4-dependent IL-1Ra production. PMID: 26972847
IL-1Ra deficiencies might promote early plaque development in 16-week-old apoE(-/-) mice. PMID: 26413013
IL-1Ra is associated with MMP-13 expression and has a novel function in such regulation without interference of the IL-1 signaling cascade. PMID: 26474296
These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
These findings demonstrate a pathogenic mechanism in which adaptive and innate immunity induce an autoimmune disease in a coordinated manner. PMID: 26108163
Leading to enhanced interleukin-1 receptor antagonist (IL-1RA) production. PMID: 25909959
IL-1Ra may have a protective effect upon EBOV infection. PMID: 26209680
IL-1ra regulates baseline GABAergic transmission in the central amygdala and is critical for the ethanol effects at these synapses. PMID: 25479427
normalization of IL-1Ra in obesity improved insulin sensitivity due to decreased inflammation in the liver and improved hepatic insulin sensitivity and these effects were independent of changes in body weight. PMID: 25244011
Loss of IL-1ra gene is associated with spontaneous arthritis. PMID: 21414240
We demonstrate that Aspergillus fumigatus galactosaminogalactan inhibits cytokines production by inducing Interleukin-1 receptor antagonist PMID: 24603878
IL-1Ra deficiency promoted the expression of inflammatory cytokines beyond IL-1 and altered the expression of genes involved in bone resorption in A. actinomycetemcomitans-infected osteoblasts. PMID: 24566623
IL-1rn(-/-) mice develop spinal abnormalities that resemble characteristic features associated with human intervertebral disc degeneration. PMID: 23396662
Modulation of the IL-1ra pathway by classical IL-6 signalling demonstrates anti-inflammatory and protective activities of IL-6 in vivo. PMID: 22980031
In the mouse ischemic heart and in a mouse cardiomyocyte cell line exposed to long-lasting hypoxia, IL-1ra bound and inhibited mitochondria-activated caspases. PMID: 23308180
Balb/c mice deficient in IL-1ra (mouse gene of IL-1RN) develop spontaneous autoimmune arthritis while DBA/1 mice deficient in IL-1ra do not PMID: 22942082
The results showed that CCR5 deficiency caused apoptotic cell death of melanoma through inhibition of NF-kappaB and upregulation of IL-1Ra. PMID: 22567084
The results showed that the local production of IL-1Ra by hepatocytes contributes to the resolution of hepatitis. The injured liver produced large amounts of IL-1Ra. The IL-1Ra isoforms were produced with different kinetics. PMID: 22539301
IL-1Ra plays an important role in suppressing local responses in eyes injected with LPS. PMID: 22267332
This gene is known to be important for immune responses and may potentially regulate alcohol consumption. PMID: 21309947
An imbalance in IL-1/IL-1Ra signaling at the IL-1R1 level modulates the severity of cardiac remodeling after acute myocardial infarction in the mouse. PMID: 22140485
Energy-sensing factors coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) and AMP-activated protein kinase control expression of inflammatory mediators in liver: induction of interleukin 1 receptor antagonist. PMID: 22117073
infection of IL-1RA(-/-) mice with Leishmania major led to significantly worsened disease outcome with larger lesions, dramatically higher parasite burdens, and decreased IFN-gamma production by antigen-specific T cells PMID: 21525884
study demonstrates that intestinal epithelia, despite not expressing IL-1beta, secrete sIL-1Ra in a TLR5-dependent manner suggesting that loss of TLR5 may promote inflammation by increasing IL-1beta activity PMID: 20844479
these results show a protective effect of the IL-1ra in vivo in TLR9-associated liver injury. PMID: 20727895
Findins define distinct roles for two major cellular sources of IL-1Ra in response to different types of systemic inflammatory stimuli in vivo. PMID: 20639493
The development of psoriasis-like lesions in IL-1R antagonist (Il1rn)-deficient mice is dependent on locally produced tumor necrosis factor (TNF), but not on IL-6 or IL-17. PMID: 20610641
TLR4-mediated interleukin-1 receptor antagonist production plays a negative regulatory role in low molecular weight hyaluronan (200 kDa)-induced mouse lung inflammation, promoting an anti-inflammatory response. PMID: 20357263
IL-1Ra deficiency in inflammatory cells induced aortic valve inflammation and TNF-alpha participates in the development of aortic stenosis in IL-1Ra(-/-) mice. PMID: 20110570
MSK1 regulates the transcription of IL-1ra in response to TLR activation in macrophages PMID: 19922413
Effect of MPC-11 myeloma and MPC-11 + IL-1 receptor antagonist treatment on mouse bone properties. PMID: 11792572
Balance between endogenous IL-1 and IL-1Ra during the primary immune response can help determine the antigen-specific effector function of T cells. PMID: 11810022
Regulation of transcription of the intracellular interleukin-1 receptor antagonist gene by AP-1 PMID: 11933077
Genetic alterations of IL-1 receptor antagonist in mice affect plasma cholesterol level and foam cell lesion size PMID: 11983917
highly expressed not only in liver and spleen, but also in white adipose tissue (WAT), where it is upregulated in obesity PMID: 12716739
Absence of IL-1Ra promoted neointimal formation in mice after arterial injury PMID: 12874179
CB1 and CB2 receptors modulate release of endogenous IL-1ra from primary cultured glial cells; endogenous IL-1ra is essential for the neuro-protective effects of CBs against excessive activation of glutamate receptors in cultured neurons PMID: 12878687
Sleep deprivation markedly increased interleukin 1 receptor antagonist, a natural (anti-inflammatory) modulator of interleukin-1. PMID: 14583241
IL-1Ra plays a role in the suppression of lesion development during early atherogenesis & in the modulation of plaque composition. PMID: 15059807
Data suggest that IL-1 receptor antagonist deficiency in T cells disrupts homeostasis of the immune system and that TNF-alpha plays an important role in activating T cells through induction of OX40. PMID: 15578092
hepatocyte-derived IL-1ra may control optimal activation and migration of inflammatory dendritic cells within the liver and thereby determine the local immune responses in granulomatous liver disease PMID: 15749858
IL-1Ra is an important regulator of adipogenesis, food intake, and energy expenditure, as seen in knockout mice. PMID: 16306368
IL-1Ra reduces insulin sensitivity in rats through a muscle-specific decrease in glucose uptake. PMID: 16385385
absence of IL-1ra may suppress TGF-beta-mediated signaling pathway, which is crucial for collagen deposition and vascular endothelial growth factor-mediated neovascularization in wound heali PMID: 16622029

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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