Recombinant Mouse IL-4 Protein

Name: Recombinant Mouse IL-4 Protein
Brand: Beta LifeScience
SKU: BL-1716NP
Availability: InStock

BL-1716NP: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant interleukin-4 (il-4) proteins, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-4 is produced by our E.coli expression system and the target gene encoding His23-Ser140 is expressed.
Accession	P07750
Synonym	Interleukin-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; IGG1 induction factor; Lymphocyte stimulatory factor 1; IL-4; BSF-1
Gene Background	Mouse Interleukin-4(IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four αhelix structure. IL4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL4 is primarily expressed by Th2biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Molecular Mass	13.4 KDa
Apmol Mass	14 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Trehalose, pH 6.5.
Endotoxin	Less than 1 EU/µg as determined by LAL test.
Purity	Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Subcellular Location	Secreted.
Protein Families	IL-4/IL-13 family
Database References	KEGG: mmu:16189 STRING: 10090.ENSMUSP00000000889 UniGene: Mm.276360

Gene Functions References

Downregulation of macrophage Irs2 by hyperinsulinemia impairs IL-4-indeuced M2a-subtype macrophage activation in obesity. PMID: 30451856
Ndfip1 preserves Treg lineage stability and immune homeostasis by preventing the expansion of highly proliferative and metabolically active Treg cells and by preventing pathological secretion of IL-4 from Treg cells PMID: 28580955
IL-4/ STAT6 signaling needs to be well adjusted to ensure proper development and function of homing Th2 cells. PMID: 29738764
By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
The VEGFR1-mediated signaling suppressed IL-4-induced Arg-1 expression. PMID: 29110610
The results obtained in the present study suggest that a signaling pathway mediated by FcRg or the FcRg-Syk axis is commonly required for innate basophil IL-4 responses under conditions mimicking encounters with allergen sources. PMID: 26703455
IL-4Delta2 did not compete with IL-4 for IL-4Ralpha binding and did not interfere with the downstream STAT-6 phosphorylation in T cells. PMID: 28917204
this study shows that IL4 and IL21 cooperate to induce the high Bcl6 protein level required for germinal center formation PMID: 28875978
The complex role of IL-4 in autoimmunity and cholangitis. PMID: 27721424
The results demonstrate that IL-4 can restore insulin sensitivity in adipocytes via mechanisms not associated with induced adipogenesis or de novo formation of lipid depots. PMID: 29738684
Interleukin 4 (IL-4) signaling prevents Chlamydia trachomatis Infection from Inducing upper genital tract (UGT) pathology. PMID: 28765368
In the lung, surfactant protein A (SP-A) enhanced interleukin-4 (IL-4)-dependent macrophage proliferation and activation, accelerating parasite clearance and reducing pulmonary injury after infection with a lung-migrating helminth. In the peritoneal cavity and liver, C1q enhancement of type 2 macrophage activation was required for liver repair after bacterial infection. PMID: 28495878
Data, including data from studies using transgenic mice, suggest that over-expression of IL4 (interleukin 4) in thyroid tissue/cells up-regulates expression of Duox1 (dual oxidase 1), Duoxa1 (dual oxidase maturation factor 1), and Slc26a4 (pendrin) in thyroid tissue/cells; expression of Slc5a5 (sodium-iodide symporter) is down-regulated. PMID: 27599561
we defined a molecular mechanism for IL-4 downregulation of involucrin in keratinocytes, which may play an important role in the pathogenesis of AD. PMID: 26918372
In this study, the effect of continuous IL-4 delivery or bioactive implant coating that constitutively releases a protein inhibitor of CCL2 signaling (7ND) on particle induced osteolysis were studied in the murine continuous femoral intramedullary particle infusion model PMID: 27114284
T follicular helper (Tfh) cells arise in tumor-draining lymph nodes where they produce an abundance of IL4. Deletion of IL4-expressing Tfh cells improves antitumor immunity, delays tumor growth, and reduces the generation of immunosuppressive myeloid cells in the lymph nodes. PMID: 27920023
Findings suggest that interleukin 4 (IL-4) affects anti-tumor immunity and constitutes an attractive therapeutic target to reduce immune suppression in the tumor microenvironment. PMID: 28733709
this study shows that environmental IL-4 plays a role in conditioning early thymic progenitors lineage choice, which would impact T cell development PMID: 28893952
this study shows that eosinophils subvert host resistance to an intracellular pathogen by instigating non-protective IL-4 in CCR2(-/-) mice PMID: 27049063
findings show that during intestinal helminth infection, IL-4 derived from T follicular helper cells is required for IgE class switching and plasmablast formation PMID: 28533444
Data suggest that Il4 (usually released from helper T-cells) induces Cox1 in macrophages at post-transcriptional level; activation of Ampk (catalytic subunit Prkaa1) by metformin blocks Il4-dependent induction of Cox1 and blocks macrophage polarization/activation. (Il4 = interleukin-4; Cox1 = cyclooxygenase 1; Ampk = AMP-activated protein kinase) PMID: 28684424
IL-4 is required for the development of ex-Foxp3 T helper 2 cells. PMID: 28507062
conclude that a state of haploinsufficiency for the Il4 gene locus is specifically relevant for IL-4-dependent IgE responses to allergens with the amount of IL-4 produced in the hemizygous condition falling close to the threshold required for switching to IgE production PMID: 28115531
priming of T helper cells by IL-6-deficient antigen-presenting dendritic cells preferentially leads to accumulation of a subset of Follicular helper T cells characterized by high expression of GATA3 and IL-4. PMID: 27474166
eosinophils drive progression of myocarditis to Inflammatory dilated cardiomyopathy (DCMi), cause severe DCMi when present in large numbers, and mediate this process through IL-4. PMID: 28302646
These data suggest that although IL-4-stimulated alternatively activated macrophages upregulate fatty acid oxidation, fatty acid oxidation is dispensable for macrophage polarization and high-fat diet-induced metabolic dysfunction. Macrophage fatty acid oxidation likely plays a correlative, rather than causative, role in systemic metabolic dysfunction. PMID: 28223293
Excessive IL-4 levels in the mesenteric lymph nodes (MLNs) directly inhibited the induction of aiTregs and caused enteropathy. The aiTregs generated in the attenuation of T cell-dependent food allergic enteropathy may function differently than aiTregs induced in a tolerance model. PMID: 28234975
this study shows that wild-type mice develop an eosinophilic Th2 airway disease in response to Alternaria alternata exposure, whereas IL-4-deficient mice exhibit a primarily neutrophilic response PMID: 27815425
Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID: 27956120
These findings indicate that IL-4, a canonical Th2 cell cytokine, can sometimes promote rather than impair Th1 cell-type immune responses PMID: 27298446
Keratinocyte gene expression is critically shaped by IL-4, altering cell fate decisions, which are likely important for the clinical manifestations and pathology of allergic skin disease PMID: 27554818
Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID: 27995420
this study shows that IL-4-mediated control of the precursor population affects the development of virus-specific CD8+ T-cell memory PMID: 27457412
IL-4 secretion by group 2 innate lymphoid cells contributes to the allergic response in food allergy by reducing allergen-specific Treg cell and activating mast cell counts PMID: 27177780
These studies clearly show a crucial role for IL-4 in the induction of airway hyperresponsiveness following Strongyloides venezuelensis infection and for IL-33/ST2 in maintaining airway hyperresponsiveness and lung Th2 responses. PMID: 27102638
we used recombinant herpes simplex virus vector S4IL4 that encode mouse il4 gene to evaluate the therapeutic potential of IL-4 in naloxone-precipitation morphine withdrawal (MW). One week after microinjection of the vector S4IL4 into the PAG LacZ or mouse IL-4 immunoreactivity in the vlPAG was visualized. ELISA assay showed that vector S4IL4 into the PAG induced the expression of IL-4 PMID: 28206989
this study shows that IL-4 is increased in the brain of T cell receptor transgenic mice, which exhibit impaired memory and adult hippocampal neurogenesis PMID: 27432189
this study shows that il-4 plays an important role in ESAT-6-induced MCP-1 production by macrophages, and suggest a pathway with significance in tuberculosis pathogenesis PMID: 27154637
indicate that Siglec-9 affects several different signaling pathways in IL-4-stimulated macrophages, which resulted in enhanced induction of Arg1 in Siglec-9-expressing RAW264 cells PMID: 26540411
Oct-1 and Oct-2 bound within the Il4 promoter region and the Th2 LCR PMID: 26840450
Loss of IL-4 promoted expression of M1 microglia/macrophage markers and impaired expression of M2 markers after transient or permanent middle cerebral artery occlusion. PMID: 26732561
These results indicate a positive role of Batf in promoting the generation of pro-allergic IL-4-producing T-follicular helper cells. PMID: 26278622
IL-4 induces miR-142-5p and downregulates miR-130a-3p in macrophages, regulating macrophage profibrogenic gene expression in chronic inflammation. PMID: 26436920
these findings underscore the important collaboration between IL-4 and IL-21 in shaping T-dependent B cells antibody responses. PMID: 26491200
IL-4 KO mice display state, but not trait, anxiety suggesting that reductions in endogenous anti-inflammatory bioactives can engender subtypes of anxiety PMID: 25772794
physiologic doses of interleukin-4 (IL-4) and interleukin-13 (IL-13) have profound anti-lymphangiogenic effects and potently impair LEC survival, proliferation, migration PMID: 26039103
Concerted activity of IgG1 antibodies and IL-4/IL-25-dependent effector cells trap helminth larvae in the tissues following vaccination with defined secreted antigens, providing sterile immunity to challenge infection. PMID: 25816012
may be an important factor in providing 1,25D3-induced protection from experimental autoimmune encephalomyelitis PMID: 25574039
IL-4-producing DCs are induced under some Th2-provoking situations, and they should play important roles in initiation of Th2 response. PMID: 26363056
RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4. PMID: 26416964

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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