Recombinant Mouse IL-5 Protein (C-6His)

Name: Recombinant Mouse IL-5 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2112NP
Availability: InStock

BL-2112NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Mouse Interleukin-5 is produced by our Mammalian expression system and the target gene encoding Met21-Gly133 is expressed with a 6His tag at the C-terminus.
Accession	P04401
Synonym	Interleukin-5; IL-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; IL5
Gene Background	IL-5 is expressed in eosinophils, NK cells, TC2 CD8+ T cells, mast cells, CD45+ CD4+ T cells, gamma delta T cells and IL-1 beta activated endothelial cells. IL-5 acts as a growth and differentiation factor for both B cells and eosinophils. Relative to B cells, IL-5 appears to induce the differentiation of activated conventional B-2 cells into Ig-secreting cells. In addition, it induces the growth of B-1 progenitors as well as IgM production by B-1 cells.IL-5 appears to perform a number of functions on eosinophils. These include the down modulation of Mac-1,the upregulation of receptors for IgA and IgG,the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release.IL-5 also promotes the growth and differentiation of eosinophils.
Molecular Mass	13.9 KDa
Apmol Mass	14-26 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location	Secreted.
Protein Families	IL-5 family
Database References	KEGG: mmu:16191 STRING: 10090.ENSMUSP00000043369 UniGene: Mm.4461

Gene Functions References

binding of IL-5 to IL-5Ralpha receptors enhances angiogenic responses by stimulating the expression of HSP70-1 via the eNOS signaling pathway. PMID: 28317868
IL-33 acts directly on bone marrow ILC2s, making them an early source of IL-5 and part of a process that is central in IL-33-driven eosinophilia. PMID: 28921511
Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
these studies establish a basal defect in eosinophilopoiesis in IL-33- and ST2-deficient mice and a mechanism whereby IL-33 supports eosinophils by driving both systemic IL-5 production and the expansion of IL-5Ralpha-expressing precursor cells PMID: 27683753
Increased production of IL-5 from Peyer's patch cells and the restored Th1-type immune response might cause the production of abnormal IgA and might induce the glomerular deposition of IgA in IGA nephropathy. PMID: 26719095
selective proliferation of IgM rheumatoid factor-secreting B-1a cells is induced by co-stimulation by the specific pathogen antigen and IL-5 in the development of MC in Capillaria hepatica-infected mice PMID: 25452118
IL5, a cytokine involved in allergic and infectious diseases, facilitates metastatic colonization through recruitment of sentinel eosinophils and regulation of other inflammatory/immune cells in the microenvironment of the distal lung. PMID: 25691457
Data (including data from knockout mice) suggest that up-regulation of IL5 production in lungs during influenza virus infection is due to infiltration of natural killer cells and alveolar macrophages into infected lung tissue. PMID: 24068930
A decrease in the levels of IL-5, IL-9, and IL-6R in the BALF. PMID: 24246030
eosinophils express CAR4 following IL-5 or allergen exposure, and that CAR4 is involved in regulating the lung transcriptome associated with allergic airway inflammation PMID: 24808371
Protection of montelukast on OVA-induced eosinophilic gastroenteritis via modulating IL-5, eotaxin-1 and MBP expression. PMID: 23855447
Together, these studies support the conclusion that surfactant protein D increases susceptibility to Cryptococcus neoformans infection by promoting Cryptococcus neoformans-driven pulmonary IL-5 and eosinophil infiltration. PMID: 24478083
IL5 induced eosinophils and cysteinyl leukotrienes are involved in the pathology of mite antigen-induced chronic asthma model. PMID: 23942524
Id3 is a key regulator of natural helper cell IL-5 production and B-1a B cell homeostasis. PMID: 24115031
Interleukin-5 plays a key role in mouse strain- dependent susceptibility to contact hypersensitivity through its effects on initiator B cells. PMID: 23711860
macrophage IL-5 is a target gene for LXR activation, and the induction of macrophage IL-5 expression can be related to LXR-inhibited atherosclerosis. PMID: 23150660
Ang II induces increased Th2 cytokines IL-5 and IL-10 early in the course of experimental abdominal aortic aneurysm formation, and inhibition of IL-5 prevents AAA formation suggesting an important role. PMID: 22459292
Sex difference in IL-5 production by splenocytes might be due, at least in part, to the sex difference in the sensitivity of CD4+ T cells to suppression by CD8+ T cells. PMID: 22627364
Invading tumor cells enhance and increase local IL-5 production from innate IL-5-producing non-T lymphoid cells residing in the intestine, peritoneal cavity and lungs of naive mice. PMID: 22174445
PARP-1 regulates Il-5 production through calpain degradation of STAT-6 in a murine asthma model. PMID: 21276008
IL-5 production by splenocytes triggered by TCR activation was higher in female mice than in male mice, and the difference might be attributable to sex differences in CD4+ and CD8+ T cell functions. PMID: 21646791
Transnasal administration of liposome- mediated IL12 could depress the expression of IL-5 in bone marrow, peripheral blood, and nasal mucosa in allergic rhinitis. PMID: 19954023
Exacerbation of oxazolone colitis by infection with the helminth Hymenolepis diminuta: involvement of IL-5 and eosinophils. PMID: 21037078
Greater antigen-induced Th2 IL-5 production by bronchial lymph node cells from female mice was associated with enhanced Th2 cell differentiation and increased expression of the Th2-specific transcription factor, GATA-3. PMID: 20337994
IL-5 production by bronchial epithelial cells can impact the microenvironment of the lung, modifying pathologic and protective immune responses in the airways PMID: 20494340
IL-5 promotes eosinophil trafficking to the esophagus PMID: 11859139
IL-5 is required for the development of tissue and marrow eosinophilia, the formation of eosinophil/basophil colony-forming units, and the early development of symptoms in experimental allergic rhinitis. PMID: 11884474
Role of IL-5 during primary and secondary immune response to acetylcholine receptor. PMID: 11960640
mechanism of synergism between eotaxin and IL5, facilitating the selective recruitment of eosinophils into sites of allergic inflammation PMID: 12083417
A putative Bcl6-binding DNA sequence which acts as a silencer element has been identified in the 3' untranslated region of IL-5 cDNA. PMID: 12097386
IL-5 alone does not account for the complexities of bronchopulmonary hyperreactivity or of eosinophil tissue trapping PMID: 12231478
effects of constitutive interleukin-5 (IL-5) expression and overabundance of eosinophils on the development and function of the mammary gland, uterus, and ovary PMID: 12620930
IL-5 appears to be required for the accumulation of eosinophils and airway hyperresponsiveness in the inflammatory lung. PMID: 12660425
The ability of IL-13 to induce eosinophilic esophagitis was abolished in STAT6-deficient mice, nearly completely ablated in IL-5-deficient mice, and significantly diminished in eotaxin-1-deficient mice. PMID: 14598258
immune effector mechanisms in murine filarial infection are dependent on both IFN-gamma and IL-5, whose synergistic effects may be mediated, at least in part, by neutrophils for the control of adult worms. PMID: 14638787
These results suggest an important role for interleukin-5, eosinophils, alphaVbeta6 integrin, and TGF-beta in airway remodeling. PMID: 14966564
Pulmonary fibrosis lesions are abolished in sensitized and allergen-exposed IL-5 receptor-null mice, whereas they are markedly accentuated in IL-5 transgenic animals. PMID: 14975941
Marked impairment of the maintenance of mature B-1 lymphocyte survival and homeostatic proliferation is demonstrated by blocking IL-5 signals. The key question is to what extent IL-5 is involved in mature B-1 cell survival and homeostatic proliferation. PMID: 15128785
IL-5 participates in the pathogenesis of ileitis in SAMP1/Yit mice. PMID: 15162425
CD4(-)c-kit(-)CD3epsilon(-)IL-2Ralpha(+) Peyer's patch cells are capable of secreting a high level of IL-5 in response to IL-2 PMID: 15214040
IL-5 links adaptive and natural immunity for epitopes of oxidized LHDL and protects from arteriosclerosis. PMID: 15286809
Anti-IL-5 was able to reduce eosinophil numbers in all tissue compartments, as well as BrdU+ eosinophils and CD34+ progenitor cells, and in all instances to a greater extent than anti-IL-9. PMID: 15823208
the increase in airway eosinophilia seen with COX inhibition is dependent on IL-5, whereas the increase in airway hyperresponsiveness is not PMID: 16339565
IL-5 is not necessary for differential splicing to occur in vivo, as all three forms of the IL-5R alpha are detected in both strains of IL-5 gene-deleted mice PMID: 16856933
It was found that MCA-induced tumor incidence and growth were significantly attenuated in IL-5 transgenic mice. PMID: 17371978
Il-5 level peaked at 7 dpi in bronchoalveolar lavage fluid. PMID: 17487773
IL-5 gene delivery suppresses sensitization to antigen (ovalbumin) by upregulating transforming growth factor beta 1-dependent signaling to CD4-expressing T cells, thus suppressing allergic airway inflammation. PMID: 17579048
cyclic AMP signals enhance histone H3 acetylation at the IL-5 promoter and the concerted binding of GATA-3 and NFATc to the promoter. PMID: 18772129
there is a reciprocal relationship between inducible nitric oxide synthase and poly(ADP-ribose) polymerase-1; expression of inducible nitric oxide synthase may be dispensable for eosinophilia after interleukin-5 production PMID: 18829681
Healing was significantly delayed in IL-5-overexpressing mice with wounds gaping wider and exhibiting impaired re-epithelialization PMID: 18839016

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