Recombinant Mouse Inactive Tyrosine-Protein Kinase 7 (PTK7) Protein (His/Tag-Free)

Name: Recombinant Mouse Inactive Tyrosine-Protein Kinase 7 (PTK7) Protein (His/Tag-Free)
Brand: Beta LifeScience
SKU: BLC-10395P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Enzymes, Featured enzyme molecules, Kinase, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Inactive Tyrosine-Protein Kinase 7 (PTK7) Protein (His/Tag-Free) is produced by our Yeast expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8BKG3
Target Symbol	PTK7
Synonyms	Ptk7; Inactive tyrosine-protein kinase 7; Protein chuzhoi; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	N-His/Tag-Free
Protein Length	Extracellular Domain
Mol. Weight	78.6kDa
Research Area	Tags & Cell Markers
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Subcellular Location	Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
Protein Families	Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database References	KEGG: mmu:71461 STRING: 10090.ENSMUSP00000043703 UniGene: Mm.181833
Tissue Specificity	Expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut and craniofacial regions among embryonic tissues.

Gene Functions References

PTK7 regulates extracellular matrix integrity and the activity levels of RAC1 and myosin II, which in turn regulates Wolffian duct morphogenesis and therefore, epididymal function. PMID: 29580943
In a Ptk7-deficient mouse strain, loss of Ptk7 leads to a diminished pool of hematopoietic stem cells but does not affect in vitro or in vivo differentiation. This is correlated with increased quiescence and reduced homing abilities of Ptk7-deficient hematopoietic stem and progenitor cells. PMID: 27183644
Data suggest that protein tyrosine kinase 7 (PTK7) is essential for Wolffian duct morphogenesis and male fertility. PMID: 26944093
data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID: 25986862
PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma cells. PMID: 24587299
Planar polarity of neural cell motility and intercalation requires Ptk7 function. PMID: 24703875
acts in conjunction with the noncanonical Wnt pathway to orient epithelial planar cell polarity through modulation of myosin II-based contractile tension between supporting cells and hair cells PMID: 22560610
The tyrosine kinase receptor, PTK7, is implicated in beta-catenin-dependent developmental processes. PMID: 21415598
These findings illustrate a novel and pivotal role for Cdx function upstream of Ptk7 and neural tube closure in vertebrates. PMID: 21350009
Chuzhoi mutation, that causes multiple abnormalities maps, to Chromosome 17 and carries a splice site mutation in Ptk7 and causes disruption of Ptk7 protein expression. PMID: 20704721
Ptk7 mRNA was expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut, and craniofacial regions among embryonic tissues, and suggest that PTK7 plays multiple roles in embryonic development. PMID: 15019986
a mutation disrupts neural tube closure and stereociliary bundle orientation, and shows genetic interactions with a mutation in the mouse Van Gogh homologue vangl2 PMID: 15229603
polarized cell behaviors underlying CE in the mouse, clearly define specific roles for planar polarity and for the novel planar cell polarity gene, Ptk7, as essential regulators of mediolateral cell intercalation during mammalian CE. PMID: 19439496

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.